Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR6968Btlr/Mmmh
Stock Number:
045078-MU
Citation ID:
RRID:MMRRC_045078-MU
Other Names:
R6968 (G1)
Major Collection:

Strain Information

Chrna6
Name: cholinergic receptor, nicotinic, alpha polypeptide 6
Synonyms: Acra6, alpha6 nAChR
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 11440
Homologene: 20888
Snap25
Name: synaptosomal-associated protein 25
Synonyms: sp, SNAP-25, GENA 70, Bdr
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20614
Homologene: 13311
Sstr2
Name: somatostatin receptor 2
Synonyms: SSTR-2, sst2, Smstr2, Smstr-2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20606
Homologene: 37427
Ppp1r13b
Name: protein phosphatase 1, regulatory subunit 13B
Synonyms: ASPP1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 21981
VEGA: 12
Homologene: 9090
Sema3f
Name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F
Synonyms: Sema IV, Semak
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 20350
Homologene: 20885
Asah2
Name: N-acylsphingosine amidohydrolase 2
Synonyms: neutral/alkaline ceramidase
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 54447
VEGA: 19
Homologene: 10310
Tnrc6a
Name: trinucleotide repeat containing 6a
Synonyms: CAGH26, 3110054G10Rik, D130023A07Rik, 2010321I05Rik, Tnrc6
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233833
Homologene: 41399
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 10,153,678 bp
  • G to T, chromosome 1 at 10,153,679 bp
  • A to T, chromosome 2 at 14,116,018 bp
  • G to A, chromosome 2 at 26,430,486 bp
  • T to C, chromosome 2 at 57,108,746 bp
  • C to T, chromosome 2 at 103,089,453 bp
  • T to C, chromosome 2 at 136,769,770 bp
  • A to G, chromosome 2 at 164,952,940 bp
  • T to A, chromosome 3 at 45,379,542 bp
  • T to C, chromosome 4 at 14,957,852 bp
  • C to T, chromosome 4 at 43,550,217 bp
  • T to A, chromosome 4 at 137,535,156 bp
  • A to T, chromosome 4 at 147,826,197 bp
  • A to G, chromosome 5 at 65,892,519 bp
  • T to A, chromosome 5 at 137,461,813 bp
  • A to G, chromosome 5 at 139,353,991 bp
  • T to C, chromosome 6 at 43,275,342 bp
  • TG to TGG, chromosome 6 at 108,664,857 bp
  • T to A, chromosome 6 at 125,108,318 bp
  • T to C, chromosome 6 at 141,487,932 bp
  • T to A, chromosome 7 at 20,049,508 bp
  • A to T, chromosome 7 at 20,787,951 bp
  • T to A, chromosome 7 at 42,788,442 bp
  • T to C, chromosome 7 at 82,643,761 bp
  • T to C, chromosome 7 at 123,182,427 bp
  • C to T, chromosome 8 at 27,406,655 bp
  • C to A, chromosome 8 at 47,713,887 bp
  • C to T, chromosome 8 at 48,236,439 bp
  • T to C, chromosome 8 at 81,844,457 bp
  • C to T, chromosome 8 at 94,548,502 bp
  • T to C, chromosome 8 at 117,377,156 bp
  • A to C, chromosome 9 at 44,297,985 bp
  • A to G, chromosome 9 at 45,854,965 bp
  • C to T, chromosome 9 at 107,691,449 bp
  • A to C, chromosome 9 at 109,330,788 bp
  • A to T, chromosome 9 at 120,560,656 bp
  • T to C, chromosome 10 at 5,117,041 bp
  • T to C, chromosome 10 at 41,673,844 bp
  • G to A, chromosome 10 at 79,540,642 bp
  • C to T, chromosome 10 at 128,671,833 bp
  • T to C, chromosome 11 at 58,891,480 bp
  • A to G, chromosome 11 at 73,799,379 bp
  • T to A, chromosome 11 at 98,584,115 bp
  • T to A, chromosome 11 at 99,481,074 bp
  • T to C, chromosome 11 at 113,624,948 bp
  • T to C, chromosome 11 at 121,260,482 bp
  • C to T, chromosome 12 at 83,951,373 bp
  • C to T, chromosome 12 at 84,789,083 bp
  • C to T, chromosome 12 at 109,594,679 bp
  • C to A, chromosome 12 at 111,833,178 bp
  • C to T, chromosome 12 at 112,914,258 bp
  • A to T, chromosome 13 at 19,336,726 bp
  • ACTTCTTCT to ACTTCT, chromosome 13 at 59,649,576 bp
  • A to G, chromosome 13 at 69,631,290 bp
  • G to T, chromosome 13 at 102,798,078 bp
  • C to T, chromosome 13 at 102,804,647 bp
  • T to C, chromosome 15 at 9,597,340 bp
  • T to A, chromosome 15 at 75,618,461 bp
  • A to G, chromosome 17 at 71,709,613 bp
  • T to C, chromosome 18 at 7,273,155 bp
  • T to C, chromosome 18 at 36,196,446 bp
  • A to C, chromosome 19 at 15,981,738 bp
  • A to T, chromosome 19 at 32,012,513 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6968 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045078-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.