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Strain Name:
C57BL/6J-MtgxR6986Btlr/Mmmh
Stock Number:
045093-MU
Citation ID:
RRID:MMRRC_045093-MU
Other Names:
R6986 (G1)
Major Collection:

Strain Information

Mtmr3
Name: myotubularin related protein 3
Synonyms: FYVE-DSP1, 1700092A20Rik, ZFYVE10
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 74302
HGNC: HGNC:7451
Homologene: 23662
Sbf2
Name: SET binding factor 2
Synonyms: SBF2, 4833411B01Rik, mMTMH1, B430219L04Rik, Mtmr13
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 319934
HGNC: HGNC:2135
Homologene: 41810
Chd7
Name: chromodomain helicase DNA binding protein 7
Synonyms: GENA 60, GENA 47, Gena 52, Cyn, A730019I05Rik, WBE1, Whi, Todo, Obt, Mt, Lda, Flo, Edy, Dz, Cycn
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 320790
Homologene: 19067
Chrna5
Name: cholinergic receptor, nicotinic, alpha polypeptide 5
Synonyms: Acra-5, Acra5
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 110835
VEGA: 9
HGNC: HGNC:1959
Homologene: 55485
Adcy9
Name: adenylate cyclase 9
Synonyms: D16Wsu65e, ACtp10
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 11515
HGNC: HGNC:240
Homologene: 868
Scn3a
Name: sodium channel, voltage-gated, type III, alpha
Synonyms: LOC381367, Nav1.3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20269
Homologene: 56005
Irf1
Name: interferon regulatory factor 1
Synonyms: Irf-1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 16362
HGNC: HGNC:6116
Homologene: 1658
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 133,897,964 bp
  • G to T, chromosome 1 at 134,993,956 bp
  • T to C, chromosome 1 at 184,841,263 bp
  • A to T, chromosome 2 at 65,508,618 bp
  • T to A, chromosome 2 at 80,520,567 bp
  • A to T, chromosome 2 at 120,097,090 bp
  • C to T, chromosome 2 at 131,522,111 bp
  • A to G, chromosome 2 at 163,627,971 bp
  • G to T, chromosome 2 at 165,833,415 bp
  • A to G, chromosome 3 at 36,655,016 bp
  • G to C, chromosome 3 at 94,487,717 bp
  • G to A, chromosome 3 at 94,614,154 bp
  • C to T, chromosome 3 at 154,961,864 bp
  • T to A, chromosome 4 at 8,859,285 bp
  • T to C, chromosome 4 at 11,204,474 bp
  • A to G, chromosome 4 at 42,868,696 bp
  • T to A, chromosome 4 at 107,070,876 bp
  • A to G, chromosome 4 at 126,747,229 bp
  • T to A, chromosome 4 at 143,993,318 bp
  • A to T, chromosome 4 at 156,218,519 bp
  • A to G, chromosome 5 at 35,723,944 bp
  • T to A, chromosome 5 at 76,994,876 bp
  • A to T, chromosome 5 at 121,868,579 bp
  • T to A, chromosome 5 at 146,493,588 bp
  • A to G, chromosome 6 at 34,839,452 bp
  • A to G, chromosome 6 at 118,464,465 bp
  • A to T, chromosome 7 at 27,192,964 bp
  • G to A, chromosome 7 at 56,106,453 bp
  • T to C, chromosome 7 at 110,330,615 bp
  • T to C, chromosome 8 at 105,458,447 bp
  • C to T, chromosome 8 at 124,384,473 bp
  • T to C, chromosome 9 at 14,357,310 bp
  • G to A, chromosome 9 at 16,021,335 bp
  • A to G, chromosome 9 at 31,096,549 bp
  • T to A, chromosome 9 at 55,006,457 bp
  • A to G, chromosome 9 at 121,712,605 bp
  • A to T, chromosome 9 at 124,439,080 bp
  • G to T, chromosome 10 at 79,480,259 bp
  • G to A, chromosome 10 at 130,075,330 bp
  • T to C, chromosome 11 at 4,489,692 bp
  • A to G, chromosome 11 at 53,774,140 bp
  • A to G, chromosome 11 at 59,614,098 bp
  • T to A, chromosome 11 at 61,214,251 bp
  • T to A, chromosome 11 at 74,892,041 bp
  • C to T, chromosome 12 at 91,533,957 bp
  • T to A, chromosome 12 at 95,780,685 bp
  • G to T, chromosome 13 at 19,190,644 bp
  • A to G, chromosome 13 at 23,535,433 bp
  • C to A, chromosome 13 at 75,761,887 bp
  • T to C, chromosome 14 at 46,832,129 bp
  • T to C, chromosome 14 at 51,039,080 bp
  • T to C, chromosome 14 at 51,365,375 bp
  • C to T, chromosome 14 at 67,694,997 bp
  • C to A, chromosome 15 at 36,175,035 bp
  • T to C, chromosome 16 at 4,311,577 bp
  • A to G, chromosome 17 at 18,136,009 bp
  • C to T, chromosome 17 at 56,269,900 bp
  • A to T, chromosome 17 at 57,509,340 bp
  • A to T, chromosome 17 at 85,985,222 bp
  • T to C, chromosome 18 at 78,857,839 bp
  • T to C, chromosome 19 at 5,279,895 bp
  • T to C, chromosome 19 at 20,824,283 bp
  • C to T, chromosome 19 at 33,382,381 bp
  • T to C, chromosome 19 at 42,078,014 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6986 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045093-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.