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Strain Name:
C57BL/6J-MtgxR7034Btlr/Mmmh
Stock Number:
045135-MU
Citation ID:
RRID:MMRRC_045135-MU
Other Names:
R7034 (G1)
Major Collection:

Strain Information

Prrx1
Name: paired related homeobox 1
Synonyms: K-2, Prx1, mHox, Pmx1, mHox, A230024N07Rik, MHox1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 18933
HGNC: HGNC:9142
Homologene: 7896
Herc3
Name: hect domain and RLD 3
Synonyms: 5730409F18Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 73998
HGNC: HGNC:4876
Homologene: 57095
Cd2ap
Name: CD2-associated protein
Synonyms: METS-1, Mets1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 12488
VEGA: 17
Homologene: 7663
Sash1
Name: SAM and SH3 domain containing 1
Synonyms: A330076K04Rik, 1100001C18Rik, 2500002E12Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 70097
Homologene: 69182
Ppcdc
Name: phosphopantothenoylcysteine decarboxylase
Synonyms: 8430432M10Rik, 1810057I13Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 66812
VEGA: 9
Homologene: 11049
Smug1
Name: single-strand selective monofunctional uracil DNA glycosylase
Synonyms: 1200013B09Rik, A930006H09Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 71726
VEGA: 15
Homologene: 8629
Gck
Name: glucokinase
Synonyms: hexokinase 4, MODY2, HK4, Gls006, Hlb62
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 103988
HGNC: HGNC:4195
Homologene: 55440
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 36,792,973 bp
  • T to A, chromosome 1 at 58,535,537 bp
  • T to C, chromosome 1 at 66,930,236 bp
  • A to T, chromosome 1 at 150,423,607 bp
  • T to C, chromosome 1 at 153,850,574 bp
  • T to A, chromosome 1 at 163,248,338 bp
  • T to A, chromosome 1 at 171,020,088 bp
  • T to C, chromosome 1 at 171,238,308 bp
  • G to T, chromosome 1 at 180,598,252 bp
  • T to A, chromosome 2 at 39,121,135 bp
  • T to G, chromosome 2 at 69,393,176 bp
  • C to A, chromosome 2 at 104,192,538 bp
  • G to T, chromosome 2 at 163,446,145 bp
  • T to C, chromosome 3 at 55,046,039 bp
  • A to G, chromosome 3 at 95,220,433 bp
  • C to T, chromosome 3 at 96,219,988 bp
  • A to T, chromosome 4 at 112,158,084 bp
  • A to G, chromosome 4 at 119,619,300 bp
  • G to A, chromosome 4 at 126,580,982 bp
  • A to G, chromosome 4 at 134,347,342 bp
  • A to T, chromosome 4 at 143,697,328 bp
  • C to A, chromosome 4 at 152,360,941 bp
  • T to C, chromosome 5 at 25,258,813 bp
  • T to C, chromosome 5 at 28,394,498 bp
  • A to T, chromosome 5 at 63,804,915 bp
  • G to A, chromosome 5 at 101,907,518 bp
  • G to A, chromosome 5 at 112,723,904 bp
  • G to A, chromosome 5 at 136,218,885 bp
  • T to A, chromosome 6 at 3,692,543 bp
  • A to T, chromosome 6 at 58,876,855 bp
  • A to G, chromosome 6 at 121,873,644 bp
  • G to A, chromosome 6 at 148,848,205 bp
  • A to G, chromosome 7 at 3,415,303 bp
  • T to C, chromosome 7 at 27,637,012 bp
  • A to G, chromosome 7 at 31,132,756 bp
  • T to C, chromosome 7 at 42,046,220 bp
  • G to T, chromosome 7 at 47,590,090 bp
  • A to T, chromosome 7 at 104,329,536 bp
  • T to C, chromosome 7 at 118,773,092 bp
  • G to A, chromosome 8 at 9,770,589 bp
  • A to G, chromosome 8 at 14,064,330 bp
  • T to C, chromosome 8 at 22,107,723 bp
  • T to A, chromosome 8 at 63,926,834 bp
  • T to C, chromosome 8 at 125,785,302 bp
  • T to C, chromosome 9 at 57,415,170 bp
  • C to T, chromosome 9 at 99,483,965 bp
  • T to C, chromosome 9 at 108,094,271 bp
  • T to A, chromosome 9 at 108,514,777 bp
  • T to C, chromosome 9 at 118,698,365 bp
  • C to A, chromosome 10 at 8,730,083 bp
  • A to T, chromosome 10 at 53,694,814 bp
  • C to T, chromosome 10 at 69,999,379 bp
  • T to C, chromosome 10 at 76,468,363 bp
  • T to C, chromosome 10 at 77,912,592 bp
  • C to T, chromosome 10 at 98,987,310 bp
  • T to A, chromosome 10 at 100,609,256 bp
  • G to A, chromosome 10 at 129,571,072 bp
  • A to G, chromosome 11 at 5,901,747 bp
  • C to A, chromosome 11 at 12,254,177 bp
  • A to T, chromosome 11 at 20,692,498 bp
  • A to G, chromosome 11 at 52,027,215 bp
  • A to G, chromosome 11 at 53,408,409 bp
  • A to T, chromosome 11 at 75,438,418 bp
  • A to G, chromosome 11 at 87,743,258 bp
  • A to C, chromosome 11 at 99,521,236 bp
  • T to A, chromosome 11 at 103,615,812 bp
  • A to T, chromosome 13 at 37,994,025 bp
  • A to T, chromosome 13 at 55,132,387 bp
  • A to G, chromosome 14 at 33,614,435 bp
  • A to C, chromosome 14 at 56,758,628 bp
  • A to G, chromosome 15 at 57,879,047 bp
  • A to G, chromosome 15 at 76,283,880 bp
  • A to G, chromosome 15 at 83,586,885 bp
  • A to G, chromosome 15 at 101,738,337 bp
  • G to A, chromosome 15 at 102,938,360 bp
  • A to G, chromosome 15 at 103,155,942 bp
  • A to G, chromosome 16 at 5,087,502 bp
  • A to T, chromosome 16 at 50,590,588 bp
  • T to A, chromosome 16 at 85,802,746 bp
  • A to T, chromosome 17 at 18,326,410 bp
  • A to T, chromosome 17 at 42,798,599 bp
  • A to T, chromosome 19 at 20,708,178 bp
  • C to A, chromosome 19 at 37,004,469 bp
  • A to G, chromosome 19 at 38,739,357 bp
  • A to G, chromosome 19 at 45,965,249 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7034 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045135-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.