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Strain Name:
C57BL/6J-MtgxR7047Btlr/Mmmh
Stock Number:
045145-MU
Citation ID:
RRID:MMRRC_045145-MU
Other Names:
R7047 (G1)
Major Collection:

Strain Information

Wnt5b
Name: wingless-type MMTV integration site family, member 5B
Synonyms: Wnt-5b
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 22419
Homologene: 22530
Adora2a
Name: adenosine A2a receptor
Synonyms: A2aR, A2AAR, AA2AR, A2a, Rs, ARA2A
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 11540
VEGA: 10
HGNC: HGNC:263
Homologene: 20166
Dbh
Name: dopamine beta hydroxylase
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 13166
HGNC: HGNC:2689
Homologene: 615
Kat2b
Name: K(lysine) acetyltransferase 2B
Synonyms: A930006P13Rik, Pcaf
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 18519
HGNC: HGNC:8638
Homologene: 20834
Cep55
Name: centrosomal protein 55
Synonyms: 2700032M20Rik, 1200008O12Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 74107
VEGA: 19
HGNC: HGNC:1161
Homologene: 10019
Phf12
Name: PHD finger protein 12
Synonyms: PF1, 2410142K10Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 268448
Homologene: 10841
Arfgef2
Name: ARF guanine nucleotide exchange factor 2
Synonyms: BIG2, E230011G24Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 99371
Homologene: 111880
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 74,551,989 bp
  • A to T, chromosome 1 at 93,285,818 bp
  • A to G, chromosome 1 at 180,696,618 bp
  • C to A, chromosome 1 at 183,087,466 bp
  • T to A, chromosome 2 at 27,165,610 bp
  • A to G, chromosome 2 at 27,928,084 bp
  • T to C, chromosome 2 at 32,721,160 bp
  • C to T, chromosome 2 at 34,773,192 bp
  • A to T, chromosome 2 at 89,055,146 bp
  • A to G, chromosome 2 at 93,739,657 bp
  • A to C, chromosome 2 at 128,615,430 bp
  • T to A, chromosome 2 at 166,851,945 bp
  • A to C, chromosome 3 at 25,436,035 bp
  • A to T, chromosome 3 at 151,835,541 bp
  • G to T, chromosome 4 at 25,007,320 bp
  • T to C, chromosome 4 at 135,549,240 bp
  • A to T, chromosome 5 at 3,670,112 bp
  • A to G, chromosome 5 at 30,917,078 bp
  • G to T, chromosome 5 at 31,275,894 bp
  • A to C, chromosome 5 at 139,758,471 bp
  • G to A, chromosome 5 at 147,546,365 bp
  • T to A, chromosome 6 at 29,323,810 bp
  • G to T, chromosome 6 at 86,388,909 bp
  • T to A, chromosome 6 at 119,448,256 bp
  • A to G, chromosome 7 at 103,629,711 bp
  • T to A, chromosome 7 at 104,768,433 bp
  • C to A, chromosome 7 at 125,491,434 bp
  • A to G, chromosome 8 at 22,938,538 bp
  • G to A, chromosome 8 at 39,642,616 bp
  • T to C, chromosome 9 at 13,620,881 bp
  • T to A, chromosome 9 at 79,853,634 bp
  • A to G, chromosome 10 at 75,326,311 bp
  • C to T, chromosome 10 at 86,858,152 bp
  • T to C, chromosome 11 at 78,013,273 bp
  • A to G, chromosome 11 at 116,603,651 bp
  • T to A, chromosome 13 at 11,824,400 bp
  • T to C, chromosome 13 at 59,712,435 bp
  • C to T, chromosome 14 at 31,336,685 bp
  • A to T, chromosome 14 at 32,660,174 bp
  • A to T, chromosome 14 at 61,005,218 bp
  • A to T, chromosome 14 at 61,213,002 bp
  • T to A, chromosome 16 at 35,267,215 bp
  • A to T, chromosome 16 at 48,982,126 bp
  • A to C, chromosome 17 at 25,919,484 bp
  • T to A, chromosome 17 at 32,396,484 bp
  • A to T, chromosome 17 at 45,533,368 bp
  • G to T, chromosome 17 at 45,638,696 bp
  • T to C, chromosome 17 at 49,452,859 bp
  • A to G, chromosome 17 at 53,663,569 bp
  • G to T, chromosome 17 at 68,461,566 bp
  • T to G, chromosome 19 at 10,984,872 bp
  • C to T, chromosome 19 at 26,669,155 bp
  • T to A, chromosome 19 at 38,060,091 bp
  • C to T, chromosome 19 at 41,086,240 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7047 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045145-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.