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Strain Name:
C57BL/6J-MtgxR7082Btlr/Mmmh
Stock Number:
045176-MU
Citation ID:
RRID:MMRRC_045176-MU
Other Names:
R7082 (G1)
Major Collection:

Strain Information

Ptf1a
Name: pancreas specific transcription factor, 1a
Synonyms: PTF1-p48, bHLHa29
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 19213
Homologene: 10304
Inpp5d
Name: inositol polyphosphate-5-phosphatase D
Synonyms: SHIP, Src homology 2 domain-containing inositol-5-phosphatase, s-SHIP, SHIP-1, SHIP1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 16331
HGNC: HGNC:6079
Homologene: 4046
Nos2
Name: nitric oxide synthase 2, inducible
Synonyms: iNOS, Nos-2, NOS-II, Nos2a
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18126
HGNC: HGNC:7873
Homologene: 55473
Cr1l
Name: complement C3b/C4b receptor 1 like
Synonyms: mCRY, Crry
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12946
Homologene: 128275
Cacybp
Name: calcyclin binding protein
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12301
Homologene: 7649
Cdc42ep4
Name: CDC42 effector protein 4
Synonyms: CEP4, Borg4, 1500041M20Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 56699
Homologene: 8110
Rgs12
Name: regulator of G-protein signaling 12
Synonyms: 4632412M04Rik, 1200016K18Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 71729
HGNC: HGNC:9994
Homologene: 2195
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 33,772,891 bp
  • G to T, chromosome 1 at 58,224,193 bp
  • A to G, chromosome 1 at 74,527,006 bp
  • C to T, chromosome 1 at 75,411,447 bp
  • T to A, chromosome 1 at 87,695,380 bp
  • T to A, chromosome 1 at 91,334,799 bp
  • G to A, chromosome 1 at 92,596,418 bp
  • G to A, chromosome 1 at 158,763,119 bp
  • A to G, chromosome 1 at 160,203,659 bp
  • A to T, chromosome 1 at 176,089,876 bp
  • T to C, chromosome 1 at 179,775,333 bp
  • A to G, chromosome 1 at 195,123,698 bp
  • G to A, chromosome 2 at 3,223,475 bp
  • A to G, chromosome 2 at 19,445,865 bp
  • T to C, chromosome 2 at 76,749,996 bp
  • T to C, chromosome 2 at 156,748,422 bp
  • T to A, chromosome 2 at 164,948,892 bp
  • G to A, chromosome 3 at 54,299,098 bp
  • T to A, chromosome 3 at 60,024,885 bp
  • A to G, chromosome 3 at 90,507,584 bp
  • A to T, chromosome 3 at 146,430,948 bp
  • C to A, chromosome 4 at 32,762,341 bp
  • G to T, chromosome 4 at 73,917,571 bp
  • C to T, chromosome 4 at 137,718,936 bp
  • C to T, chromosome 4 at 153,959,764 bp
  • A to G, chromosome 5 at 34,966,706 bp
  • A to G, chromosome 5 at 90,772,992 bp
  • A to G, chromosome 5 at 100,804,404 bp
  • G to T, chromosome 6 at 38,332,393 bp
  • A to T, chromosome 6 at 48,478,609 bp
  • A to T, chromosome 6 at 52,179,280 bp
  • T to A, chromosome 6 at 116,328,376 bp
  • C to T, chromosome 7 at 3,895,511 bp
  • A to G, chromosome 7 at 5,115,559 bp
  • A to T, chromosome 7 at 27,878,758 bp
  • T to C, chromosome 7 at 58,658,819 bp
  • A to G, chromosome 7 at 86,225,232 bp
  • T to A, chromosome 7 at 90,731,984 bp
  • T to C, chromosome 7 at 101,508,096 bp
  • T to C, chromosome 7 at 102,949,248 bp
  • C to A, chromosome 7 at 103,358,288 bp
  • T to C, chromosome 7 at 144,410,359 bp
  • G to T, chromosome 8 at 13,320,847 bp
  • A to T, chromosome 8 at 67,904,148 bp
  • A to G, chromosome 8 at 105,773,946 bp
  • A to G, chromosome 8 at 110,089,705 bp
  • A to G, chromosome 8 at 125,995,548 bp
  • A to G, chromosome 9 at 13,833,012 bp
  • G to A, chromosome 9 at 37,666,617 bp
  • T to A, chromosome 9 at 67,883,453 bp
  • A to G, chromosome 9 at 105,931,239 bp
  • A to G, chromosome 10 at 36,832,837 bp
  • A to T, chromosome 10 at 40,867,873 bp
  • A to T, chromosome 10 at 60,775,088 bp
  • A to G, chromosome 10 at 62,954,317 bp
  • A to T, chromosome 10 at 80,060,090 bp
  • G to C, chromosome 10 at 80,835,791 bp
  • C to T, chromosome 10 at 107,708,730 bp
  • T to A, chromosome 10 at 129,607,547 bp
  • T to A, chromosome 10 at 129,607,548 bp
  • C to T, chromosome 10 at 129,625,765 bp
  • C to T, chromosome 11 at 61,377,941 bp
  • C to A, chromosome 11 at 78,928,579 bp
  • T to A, chromosome 11 at 97,033,082 bp
  • G to A, chromosome 11 at 100,203,341 bp
  • C to A, chromosome 11 at 102,147,472 bp
  • T to C, chromosome 11 at 107,086,747 bp
  • T to C, chromosome 11 at 113,729,118 bp
  • G to A, chromosome 12 at 81,724,702 bp
  • A to T, chromosome 12 at 85,746,954 bp
  • A to G, chromosome 13 at 3,933,050 bp
  • T to A, chromosome 13 at 33,154,407 bp
  • T to C, chromosome 13 at 55,006,897 bp
  • G to T, chromosome 13 at 67,071,136 bp
  • A to G, chromosome 13 at 112,504,032 bp
  • C to A, chromosome 14 at 61,210,517 bp
  • T to A, chromosome 14 at 72,442,712 bp
  • A to C, chromosome 15 at 34,584,663 bp
  • T to C, chromosome 15 at 39,077,100 bp
  • G to A, chromosome 15 at 80,648,498 bp
  • A to C, chromosome 15 at 100,791,027 bp
  • T to C, chromosome 16 at 19,982,883 bp
  • T to C, chromosome 17 at 25,234,720 bp
  • G to T, chromosome 17 at 27,890,065 bp
  • A to T, chromosome 17 at 35,621,201 bp
  • A to T, chromosome 17 at 74,021,520 bp
  • A to G, chromosome 17 at 86,057,732 bp
  • A to G, chromosome 18 at 37,266,991 bp
  • A to G, chromosome 18 at 64,504,354 bp
  • G to T, chromosome 19 at 6,171,375 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7082 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045176-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.