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Strain Name:
C57BL/6J-MtgxR7085Btlr/Mmmh
Stock Number:
045179-MU
Citation ID:
RRID:MMRRC_045179-MU
Other Names:
R7085 (G1)
Major Collection:

Strain Information

Rtn4rl1
Name: reticulon 4 receptor-like 1
Synonyms: Ngr3, Ngrh2, Ngrl2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 237847
Homologene: 18611
Bclaf1
Name: BCL2-associated transcription factor 1
Synonyms: 2700025J07Rik, 2810454G14Rik, 2610102K23Rik, 5730534O06Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 72567
Homologene: 8832
Prex2
Name: phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2
Synonyms: 6230420N16Rik, C030045D06Rik, Depdc2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 109294
Homologene: 23523
Polr2a
Name: polymerase (RNA) II (DNA directed) polypeptide A
Synonyms: 220kDa, Rpo2-1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20020
HGNC: HGNC:9187
Homologene: 721
Btaf1
Name: B-TFIID TATA-box binding protein associated factor 1
Synonyms: E430027O22Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 107182
Homologene: 31978
Ddx1
Name: DEAD box helicase 1
Synonyms: DEAD (Asp-Glu-Ala-Asp) box polypeptide 1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 104721
VEGA: 12
HGNC: HGNC:2734
Homologene: 3627
Sbno1
Name: strawberry notch 1
Synonyms: sno, 9330180L10Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 243272
Homologene: 10055
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 11,098,588 bp
  • A to G, chromosome 1 at 26,683,465 bp
  • T to A, chromosome 1 at 45,911,528 bp
  • G to A, chromosome 1 at 73,925,462 bp
  • A to T, chromosome 1 at 154,473,746 bp
  • A to T, chromosome 1 at 156,432,331 bp
  • T to C, chromosome 1 at 164,302,324 bp
  • T to A, chromosome 1 at 174,117,660 bp
  • T to A, chromosome 2 at 35,165,792 bp
  • T to A, chromosome 2 at 87,607,406 bp
  • T to C, chromosome 3 at 40,916,909 bp
  • T to A, chromosome 3 at 94,072,632 bp
  • A to T, chromosome 3 at 96,598,629 bp
  • T to C, chromosome 3 at 98,450,394 bp
  • C to A, chromosome 3 at 101,455,489 bp
  • C to A, chromosome 3 at 102,760,954 bp
  • A to G, chromosome 4 at 43,361,835 bp
  • C to T, chromosome 4 at 52,910,961 bp
  • T to A, chromosome 4 at 98,779,946 bp
  • T to A, chromosome 4 at 126,163,091 bp
  • T to C, chromosome 4 at 136,897,780 bp
  • T to A, chromosome 4 at 148,158,743 bp
  • C to A, chromosome 5 at 21,915,087 bp
  • A to T, chromosome 5 at 29,361,092 bp
  • G to A, chromosome 5 at 74,628,185 bp
  • G to A, chromosome 5 at 124,381,720 bp
  • A to T, chromosome 5 at 143,949,284 bp
  • A to G, chromosome 5 at 150,438,749 bp
  • A to G, chromosome 6 at 30,643,620 bp
  • T to C, chromosome 6 at 52,316,692 bp
  • A to G, chromosome 6 at 102,165,401 bp
  • T to C, chromosome 6 at 125,226,488 bp
  • T to A, chromosome 7 at 28,331,489 bp
  • A to G, chromosome 7 at 80,276,089 bp
  • A to T, chromosome 7 at 80,326,334 bp
  • A to T, chromosome 7 at 83,622,145 bp
  • T to A, chromosome 7 at 105,715,261 bp
  • A to T, chromosome 7 at 118,983,632 bp
  • A to G, chromosome 7 at 128,126,857 bp
  • T to A, chromosome 8 at 24,558,196 bp
  • A to T, chromosome 8 at 36,104,237 bp
  • G to A, chromosome 8 at 70,302,483 bp
  • A to G, chromosome 8 at 110,603,330 bp
  • A to G, chromosome 8 at 122,490,894 bp
  • T to A, chromosome 9 at 18,644,849 bp
  • G to A, chromosome 9 at 20,054,936 bp
  • C to A, chromosome 9 at 39,956,512 bp
  • T to C, chromosome 9 at 40,277,098 bp
  • A to C, chromosome 9 at 64,998,243 bp
  • A to G, chromosome 9 at 106,901,887 bp
  • T to C, chromosome 10 at 20,322,022 bp
  • T to A, chromosome 10 at 24,870,121 bp
  • T to C, chromosome 10 at 29,224,480 bp
  • T to G, chromosome 10 at 79,892,492 bp
  • A to G, chromosome 11 at 23,363,097 bp
  • T to A, chromosome 11 at 69,369,201 bp
  • A to G, chromosome 11 at 69,743,880 bp
  • A to G, chromosome 11 at 75,265,224 bp
  • G to T, chromosome 11 at 78,058,074 bp
  • T to A, chromosome 11 at 99,986,285 bp
  • C to A, chromosome 11 at 116,064,807 bp
  • T to C, chromosome 12 at 8,922,113 bp
  • A to C, chromosome 12 at 13,229,355 bp
  • G to T, chromosome 12 at 13,285,258 bp
  • T to C, chromosome 13 at 15,715,062 bp
  • T to C, chromosome 13 at 24,872,302 bp
  • G to A, chromosome 13 at 49,520,902 bp
  • G to T, chromosome 13 at 104,858,306 bp
  • A to T, chromosome 14 at 50,362,656 bp
  • G to A, chromosome 15 at 57,249,649 bp
  • G to T, chromosome 15 at 75,747,687 bp
  • G to A, chromosome 17 at 44,814,192 bp
  • A to G, chromosome 17 at 71,274,105 bp
  • A to T, chromosome 17 at 71,365,219 bp
  • A to G, chromosome 18 at 61,748,814 bp
  • A to T, chromosome 18 at 73,781,058 bp
  • G to A, chromosome 19 at 5,424,787 bp
  • T to C, chromosome 19 at 6,960,133 bp
  • T to C, chromosome 19 at 36,972,918 bp
  • A to G, chromosome 19 at 47,170,662 bp
  • T to A, chromosome 19 at 57,691,857 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7085 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045179-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.