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Strain Name:
C57BL/6J-MtgxR7136Btlr/Mmmh
Stock Number:
045220-MU
Citation ID:
RRID:MMRRC_045220-MU
Other Names:
R7136 (G1)
Major Collection:

Strain Information

Ptk2
Name: PTK2 protein tyrosine kinase 2
Synonyms: Fadk, FAK, FRNK
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 14083
VEGA: 15
HGNC: HGNC:9611
Homologene: 7314
Nos1
Name: nitric oxide synthase 1, neuronal
Synonyms: nNOS, bNOS, Nos-1, NO, 2310005C01Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 18125
HGNC: HGNC:7872
Homologene: 37327
Rgs14
Name: regulator of G-protein signaling 14
Synonyms: Rap1/rap2 interacting protein
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 51791
HGNC: HGNC:9996
Homologene: 4735
Wdr82
Name: WD repeat domain containing 82
Synonyms: 9430077D24Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 77305
Homologene: 42951
Kdm3a
Name: lysine (K)-specific demethylase 3A
Synonyms: 1700105C21Rik, Tsga, C230043E16Rik, Jmjd1, Jmjd1a
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 104263
Homologene: 10196
Wasf1
Name: WASP family, member 1
Synonyms: WAVE-1, Scar, WAVE
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 83767
Homologene: 2920
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 2 at 76,836,560 bp
  • T to C, chromosome 2 at 78,913,741 bp
  • T to G, chromosome 2 at 126,124,028 bp
  • A to T, chromosome 2 at 143,949,680 bp
  • A to T, chromosome 2 at 170,494,143 bp
  • T to C, chromosome 3 at 19,231,094 bp
  • C to T, chromosome 3 at 19,985,658 bp
  • C to T, chromosome 3 at 97,694,063 bp
  • T to C, chromosome 3 at 113,563,599 bp
  • T to C, chromosome 4 at 43,669,845 bp
  • A to G, chromosome 4 at 134,470,777 bp
  • T to C, chromosome 4 at 138,317,458 bp
  • A to T, chromosome 5 at 5,466,631 bp
  • A to T, chromosome 5 at 24,332,991 bp
  • G to T, chromosome 5 at 117,895,860 bp
  • A to G, chromosome 5 at 118,721,522 bp
  • T to G, chromosome 5 at 135,149,828 bp
  • A to G, chromosome 5 at 138,562,348 bp
  • C to A, chromosome 5 at 139,108,608 bp
  • C to T, chromosome 6 at 40,900,053 bp
  • T to C, chromosome 6 at 57,210,254 bp
  • T to G, chromosome 6 at 58,684,340 bp
  • G to A, chromosome 6 at 71,611,780 bp
  • A to G, chromosome 7 at 4,477,404 bp
  • T to A, chromosome 7 at 28,883,107 bp
  • A to T, chromosome 7 at 47,427,186 bp
  • A to G, chromosome 7 at 48,801,332 bp
  • T to C, chromosome 7 at 79,793,158 bp
  • T to A, chromosome 7 at 86,228,767 bp
  • T to C, chromosome 7 at 127,108,812 bp
  • C to A, chromosome 7 at 127,750,045 bp
  • T to C, chromosome 8 at 95,103,449 bp
  • T to A, chromosome 8 at 119,441,437 bp
  • A to G, chromosome 9 at 16,378,185 bp
  • C to T, chromosome 9 at 52,086,445 bp
  • T to A, chromosome 9 at 89,991,598 bp
  • A to G, chromosome 9 at 106,171,333 bp
  • T to C, chromosome 9 at 108,512,772 bp
  • T to C, chromosome 10 at 13,505,921 bp
  • A to T, chromosome 10 at 40,926,591 bp
  • T to C, chromosome 10 at 78,847,781 bp
  • C to T, chromosome 10 at 127,558,622 bp
  • C to T, chromosome 11 at 4,148,592 bp
  • T to C, chromosome 11 at 44,408,030 bp
  • C to T, chromosome 11 at 69,348,438 bp
  • G to A, chromosome 11 at 84,877,759 bp
  • A to G, chromosome 11 at 106,205,004 bp
  • A to G, chromosome 11 at 107,099,715 bp
  • G to T, chromosome 12 at 8,538,895 bp
  • A to G, chromosome 13 at 55,379,695 bp
  • A to G, chromosome 13 at 67,842,992 bp
  • C to T, chromosome 14 at 24,461,815 bp
  • T to C, chromosome 14 at 31,298,656 bp
  • T to A, chromosome 14 at 40,840,252 bp
  • T to A, chromosome 14 at 101,920,539 bp
  • T to C, chromosome 14 at 105,144,267 bp
  • G to A, chromosome 15 at 73,221,809 bp
  • A to G, chromosome 15 at 76,370,431 bp
  • C to A, chromosome 15 at 98,911,491 bp
  • G to T, chromosome 16 at 20,734,522 bp
  • C to T, chromosome 16 at 59,148,964 bp
  • T to C, chromosome 16 at 73,956,550 bp
  • G to T, chromosome 16 at 87,483,171 bp
  • T to C, chromosome 17 at 24,613,280 bp
  • T to C, chromosome 17 at 33,965,288 bp
  • T to C, chromosome 17 at 35,320,627 bp
  • T to A, chromosome 18 at 86,716,672 bp
  • A to G, chromosome 19 at 5,706,076 bp
  • A to G, chromosome 19 at 43,837,460 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7136 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045220-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.