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Strain Name:
C57BL/6J-MtgxR7141Btlr/Mmmh
Stock Number:
045221-MU
Citation ID:
RRID:MMRRC_045221-MU
Other Names:
R7141 (G1)
Major Collection:

Strain Information

Met
Name: met proto-oncogene
Synonyms: HGF receptor, c-Met, Par4
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 17295
HGNC: HGNC:7029
Homologene: 206
Btbd17
Name: BTB domain containing 17
Synonyms: 1500005I02Rik, Tango10a
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 72014
Homologene: 46324
Rgs3
Name: regulator of G-protein signaling 3
Synonyms: 4930506N09Rik, C2pa, PDZ-RGS3, RGS3S, C2PA-RGS3
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 50780
HGNC: HGNC:9999
Homologene: 32440
Myh10
Name: myosin, heavy polypeptide 10, non-muscle
Synonyms: nonmuscle myosin heavy chain II-B, NMHC-B, SMemb, nonmuscle myosin heavy chain IIB, 9330167F11Rik, 5730504C04Rik, NMHC II-B, Myhn2, Myosin IIB, Myhn-2, myosin IIB, Fltn, Fltn
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 77579
HGNC: HGNC:7568
Homologene: 55941
Map4
Name: microtubule-associated protein 4
Synonyms: MAP 4, Mtap4
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 17758
HGNC: HGNC:6862
Homologene: 1780
Zfp638
Name: zinc finger protein 638
Synonyms: Np220, Zfml
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 18139
Homologene: 7447
Appbp2
Name: amyloid beta precursor protein binding protein 2
Synonyms: PAT1, 1300003O07Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 66884
HGNC: HGNC:622
Homologene: 31378
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 36,553,020 bp
  • A to C, chromosome 1 at 74,284,111 bp
  • T to C, chromosome 1 at 88,407,328 bp
  • G to T, chromosome 1 at 155,914,748 bp
  • A to G, chromosome 1 at 182,448,508 bp
  • C to T, chromosome 2 at 3,225,152 bp
  • A to G, chromosome 2 at 24,974,456 bp
  • C to A, chromosome 2 at 31,360,896 bp
  • A to T, chromosome 2 at 103,073,314 bp
  • A to G, chromosome 3 at 116,753,286 bp
  • A to G, chromosome 4 at 62,690,487 bp
  • T to A, chromosome 4 at 137,552,116 bp
  • T to C, chromosome 5 at 14,679,257 bp
  • A to G, chromosome 5 at 73,629,977 bp
  • G to T, chromosome 5 at 114,769,698 bp
  • T to A, chromosome 6 at 17,527,155 bp
  • C to T, chromosome 6 at 18,380,468 bp
  • G to T, chromosome 6 at 29,487,512 bp
  • T to C, chromosome 6 at 42,280,395 bp
  • A to T, chromosome 6 at 70,572,981 bp
  • T to C, chromosome 6 at 83,867,199 bp
  • T to A, chromosome 6 at 129,583,166 bp
  • A to T, chromosome 7 at 19,054,092 bp
  • A to G, chromosome 7 at 48,447,687 bp
  • G to C, chromosome 7 at 85,558,836 bp
  • A to G, chromosome 8 at 56,539,851 bp
  • A to G, chromosome 8 at 104,113,001 bp
  • A to T, chromosome 8 at 110,030,839 bp
  • T to C, chromosome 9 at 99,321,440 bp
  • T to C, chromosome 9 at 109,978,870 bp
  • T to C, chromosome 9 at 114,404,968 bp
  • A to G, chromosome 10 at 81,273,055 bp
  • G to T, chromosome 10 at 81,487,849 bp
  • G to A, chromosome 10 at 89,498,229 bp
  • A to G, chromosome 11 at 68,802,139 bp
  • A to T, chromosome 11 at 73,160,648 bp
  • A to T, chromosome 11 at 85,191,751 bp
  • T to C, chromosome 11 at 87,600,613 bp
  • A to G, chromosome 11 at 87,774,697 bp
  • G to T, chromosome 11 at 98,427,309 bp
  • A to C, chromosome 11 at 101,072,230 bp
  • T to C, chromosome 11 at 114,791,815 bp
  • T to A, chromosome 13 at 17,644,787 bp
  • C to T, chromosome 13 at 23,034,669 bp
  • A to G, chromosome 13 at 81,492,501 bp
  • T to A, chromosome 13 at 100,219,830 bp
  • T to G, chromosome 14 at 6,301,807 bp
  • G to T, chromosome 14 at 51,655,882 bp
  • T to A, chromosome 15 at 99,283,892 bp
  • C to T, chromosome 16 at 45,742,806 bp
  • G to A, chromosome 16 at 58,797,408 bp
  • T to C, chromosome 17 at 3,159,182 bp
  • A to G, chromosome 17 at 33,018,795 bp
  • T to C, chromosome 17 at 40,952,839 bp
  • T to C, chromosome 17 at 57,025,602 bp
  • A to T, chromosome 18 at 63,145,110 bp
  • G to A, chromosome 19 at 4,102,787 bp
  • A to G, chromosome 19 at 25,181,620 bp
  • T to C, chromosome 19 at 43,736,747 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7141 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045221-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.