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Strain Name:
C57BL/6J-MtgxR7135Btlr/Mmmh
Stock Number:
045247-MU
Citation ID:
RRID:MMRRC_045247-MU
Other Names:
R7135 (G1)
Major Collection:

Strain Information

Espl1
Name: extra spindle pole bodies 1, separase
Synonyms: separase, SSE, ESP1, PRCE, PRCE, Cerp
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 105988
VEGA: 15
Homologene: 32151
Sim1
Name: single-minded family bHLH transcription factor 1
Synonyms: bHLHe14
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 20464
VEGA: 10
Homologene: 3715
Pcf11
Name: PCF11 cleavage and polyadenylation factor subunit
Synonyms: 5730417B17Rik, 2500001H09Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 74737
Homologene: 32282
Pecam1
Name: platelet/endothelial cell adhesion molecule 1
Synonyms: PECAM-1, Cd31
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18613
HGNC: HGNC:8823
Homologene: 47925
Birc2
Name: baculoviral IAP repeat-containing 2
Synonyms: mcIAP1, MIAP1, IAP1, MIHB, Api1, cIAP1, cIAP-1, HIAP1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 11797
HGNC: HGNC:590
Homologene: 900
Pcbp1
Name: poly(rC) binding protein 1
Synonyms: [a]CP-1, WBP17, hnRNP E1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 23983
HGNC: HGNC:8647
Homologene: 68506
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 4,348,168 bp
  • C to T, chromosome 1 at 10,088,936 bp
  • T to C, chromosome 1 at 30,831,109 bp
  • T to C, chromosome 1 at 46,139,710 bp
  • C to T, chromosome 1 at 46,533,208 bp
  • T to C, chromosome 1 at 79,609,202 bp
  • T to A, chromosome 1 at 82,747,091 bp
  • C to A, chromosome 1 at 139,243,367 bp
  • A to T, chromosome 1 at 153,875,166 bp
  • T to A, chromosome 1 at 167,466,450 bp
  • C to A, chromosome 1 at 182,975,523 bp
  • G to A, chromosome 2 at 28,848,306 bp
  • A to G, chromosome 2 at 57,112,249 bp
  • G to A, chromosome 2 at 104,084,447 bp
  • T to A, chromosome 2 at 110,616,714 bp
  • T to A, chromosome 2 at 111,307,020 bp
  • T to C, chromosome 2 at 150,955,604 bp
  • C to A, chromosome 2 at 153,714,952 bp
  • T to C, chromosome 3 at 35,995,818 bp
  • A to T, chromosome 3 at 82,363,458 bp
  • A to G, chromosome 3 at 108,800,755 bp
  • A to T, chromosome 3 at 142,311,922 bp
  • A to G, chromosome 4 at 116,105,830 bp
  • A to T, chromosome 4 at 143,810,663 bp
  • A to G, chromosome 4 at 156,223,335 bp
  • C to T, chromosome 5 at 11,505,231 bp
  • A to T, chromosome 5 at 21,976,596 bp
  • C to T, chromosome 5 at 101,915,437 bp
  • T to C, chromosome 5 at 111,280,007 bp
  • G to T, chromosome 5 at 142,787,817 bp
  • A to G, chromosome 6 at 18,448,447 bp
  • A to G, chromosome 6 at 48,416,299 bp
  • A to T, chromosome 6 at 55,171,028 bp
  • T to C, chromosome 6 at 68,704,743 bp
  • A to T, chromosome 6 at 86,525,506 bp
  • T to G, chromosome 7 at 21,272,402 bp
  • T to A, chromosome 7 at 31,360,214 bp
  • A to T, chromosome 7 at 92,657,316 bp
  • C to T, chromosome 7 at 99,695,063 bp
  • T to C, chromosome 7 at 108,124,574 bp
  • T to A, chromosome 7 at 120,773,030 bp
  • T to C, chromosome 7 at 129,628,106 bp
  • A to T, chromosome 8 at 39,589,424 bp
  • T to A, chromosome 8 at 88,744,892 bp
  • C to T, chromosome 8 at 119,402,477 bp
  • A to C, chromosome 9 at 7,818,761 bp
  • G to T, chromosome 9 at 45,338,345 bp
  • G to T, chromosome 9 at 99,234,443 bp
  • A to G, chromosome 10 at 5,233,409 bp
  • A to T, chromosome 10 at 6,830,203 bp
  • A to G, chromosome 10 at 41,673,859 bp
  • A to G, chromosome 10 at 50,895,927 bp
  • G to A, chromosome 10 at 52,391,719 bp
  • T to A, chromosome 10 at 100,341,727 bp
  • T to A, chromosome 11 at 30,998,501 bp
  • T to C, chromosome 11 at 49,804,955 bp
  • T to C, chromosome 11 at 70,603,503 bp
  • T to C, chromosome 11 at 83,297,642 bp
  • C to A, chromosome 11 at 105,935,567 bp
  • T to C, chromosome 11 at 106,689,031 bp
  • C to T, chromosome 11 at 115,930,672 bp
  • A to T, chromosome 12 at 8,728,952 bp
  • T to C, chromosome 12 at 36,196,312 bp
  • T to A, chromosome 12 at 108,186,475 bp
  • C to T, chromosome 13 at 9,877,801 bp
  • T to C, chromosome 13 at 17,302,009 bp
  • T to C, chromosome 13 at 24,741,506 bp
  • A to T, chromosome 13 at 38,179,073 bp
  • T to C, chromosome 13 at 100,300,419 bp
  • G to A, chromosome 14 at 26,778,912 bp
  • T to G, chromosome 14 at 26,801,413 bp
  • T to A, chromosome 14 at 43,010,493 bp
  • T to A, chromosome 14 at 43,300,788 bp
  • T to C, chromosome 14 at 75,321,721 bp
  • T to A, chromosome 15 at 44,584,978 bp
  • C to A, chromosome 15 at 79,109,424 bp
  • T to A, chromosome 15 at 96,420,328 bp
  • T to A, chromosome 15 at 102,319,524 bp
  • T to A, chromosome 16 at 18,545,168 bp
  • T to C, chromosome 16 at 22,500,382 bp
  • T to A, chromosome 17 at 45,508,961 bp
  • A to T, chromosome 17 at 66,944,288 bp
  • G to T, chromosome 18 at 22,517,701 bp
  • G to C, chromosome 18 at 22,517,702 bp
  • G to A, chromosome 18 at 33,107,943 bp
  • T to A, chromosome 19 at 12,833,319 bp
  • T to A, chromosome 19 at 41,332,321 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7135 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045247-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.