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Strain Name:
C57BL/6J-MtgxR7161Btlr/Mmmh
Stock Number:
045260-MU
Citation ID:
RRID:MMRRC_045260-MU
Other Names:
R7161 (G1)
Major Collection:

Strain Information

Spats1
Name: spermatogenesis associated, serine-rich 1
Synonyms: 1700011H05Rik, Srsp1, 4933400B06Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 71020
VEGA: 17
Homologene: 12376
Herc4
Name: hect domain and RLD 4
Synonyms: 4921531D01Rik, 1700056O17Rik, 9530080M15Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 67345
VEGA: 10
Homologene: 56715
Knl1
Name: kinetochore scaffold 1
Synonyms: 2310043D08Rik, 5730505K17Rik, Casc5
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 76464
Homologene: 44890
Rab11fip3
Name: RAB11 family interacting protein 3 (class II)
Synonyms: Rab11-FIP3, D030060O14Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 215445
Homologene: 49396
Afdn
Name: afadin, adherens junction formation factor
Synonyms: AF6, Afadin, S-afadin, I-afadin, 5033403D15Rik, Mllt4
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 17356
HGNC: HGNC:7137
Homologene: 21202
Slc9a8
Name: solute carrier family 9 (sodium/hydrogen exchanger), member 8
Synonyms: NHE8, 1200006P13Rik, 6430709P13Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 77031
Homologene: 75041
Itpr1
Name: inositol 1,4,5-trisphosphate receptor 1
Synonyms: P400, IP3R1, Itpr-1, Ip3r, Pcp-1, opt, InsP3R type I, Pcp1, wblo
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 16438
HGNC: HGNC:6180
Homologene: 1673
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 93,310,905 bp
  • T to C, chromosome 1 at 107,273,162 bp
  • A to G, chromosome 1 at 133,106,112 bp
  • A to T, chromosome 1 at 153,226,454 bp
  • C to A, chromosome 1 at 166,487,010 bp
  • A to G, chromosome 1 at 180,170,341 bp
  • A to G, chromosome 1 at 181,203,130 bp
  • A to G, chromosome 1 at 183,087,625 bp
  • A to T, chromosome 2 at 25,363,280 bp
  • A to T, chromosome 2 at 52,271,592 bp
  • A to G, chromosome 2 at 76,812,244 bp
  • A to G, chromosome 2 at 79,865,214 bp
  • A to C, chromosome 2 at 86,890,649 bp
  • A to G, chromosome 2 at 112,262,046 bp
  • A to G, chromosome 2 at 118,626,053 bp
  • A to G, chromosome 2 at 119,070,785 bp
  • C to A, chromosome 2 at 130,806,788 bp
  • A to T, chromosome 2 at 154,313,615 bp
  • A to T, chromosome 2 at 167,465,383 bp
  • A to T, chromosome 3 at 5,244,083 bp
  • A to G, chromosome 3 at 14,645,208 bp
  • A to G, chromosome 3 at 106,394,412 bp
  • A to G, chromosome 4 at 58,128,859 bp
  • T to C, chromosome 4 at 59,329,581 bp
  • G to A, chromosome 4 at 103,096,616 bp
  • G to A, chromosome 4 at 137,514,719 bp
  • C to T, chromosome 5 at 45,498,467 bp
  • C to G, chromosome 5 at 93,273,343 bp
  • A to T, chromosome 5 at 121,607,373 bp
  • T to A, chromosome 5 at 124,546,920 bp
  • C to A, chromosome 5 at 134,135,190 bp
  • T to C, chromosome 5 at 145,286,441 bp
  • A to T, chromosome 6 at 41,660,536 bp
  • T to A, chromosome 6 at 70,215,778 bp
  • T to A, chromosome 6 at 95,126,696 bp
  • C to T, chromosome 6 at 108,386,640 bp
  • T to C, chromosome 6 at 124,705,749 bp
  • A to G, chromosome 7 at 79,617,441 bp
  • A to T, chromosome 7 at 80,380,861 bp
  • A to T, chromosome 7 at 112,954,500 bp
  • T to A, chromosome 7 at 127,719,304 bp
  • T to G, chromosome 8 at 70,631,342 bp
  • C to T, chromosome 9 at 8,087,399 bp
  • T to C, chromosome 9 at 43,139,363 bp
  • T to A, chromosome 9 at 106,368,915 bp
  • T to G, chromosome 9 at 109,225,980 bp
  • A to G, chromosome 10 at 5,802,043 bp
  • T to A, chromosome 10 at 63,308,415 bp
  • T to C, chromosome 10 at 79,919,489 bp
  • T to A, chromosome 11 at 65,855,372 bp
  • G to A, chromosome 11 at 84,519,872 bp
  • A to G, chromosome 11 at 96,817,720 bp
  • A to G, chromosome 11 at 101,174,278 bp
  • T to A, chromosome 11 at 110,074,142 bp
  • C to T, chromosome 11 at 115,616,547 bp
  • C to G, chromosome 11 at 116,332,408 bp
  • T to A, chromosome 12 at 74,897,709 bp
  • T to C, chromosome 13 at 60,696,395 bp
  • A to T, chromosome 13 at 74,352,877 bp
  • T to C, chromosome 15 at 9,717,603 bp
  • T to C, chromosome 15 at 100,636,245 bp
  • A to G, chromosome 16 at 23,115,433 bp
  • T to C, chromosome 17 at 6,065,076 bp
  • T to C, chromosome 17 at 13,081,746 bp
  • T to A, chromosome 17 at 13,888,946 bp
  • C to A, chromosome 17 at 26,069,090 bp
  • T to A, chromosome 17 at 45,449,169 bp
  • T to A, chromosome 17 at 66,809,627 bp
  • A to T, chromosome 18 at 37,815,663 bp
  • A to T, chromosome 18 at 61,281,525 bp
  • A to G, chromosome 18 at 62,614,955 bp
  • A to G, chromosome 18 at 65,812,732 bp
  • G to A, chromosome 18 at 67,229,771 bp
  • T to A, chromosome 19 at 41,069,935 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7161 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045260-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.