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Strain Name:
C57BL/6J-MtgxR7168Btlr/Mmmh
Stock Number:
045263-MU
Citation ID:
RRID:MMRRC_045263-MU
Other Names:
R7168 (G1)
Major Collection:

Strain Information

Slc1a3
Name: solute carrier family 1 (glial high affinity glutamate transporter), member 3
Synonyms: MGluT1, Eaat1, B430115D02Rik, GLAST, Gmt1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 20512
Homologene: 20882
Ptch1
Name: patched 1
Synonyms: Ptc, Patched 1, Ptc1, A230106A15Rik, wig
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 19206
HGNC: HGNC:9585
Homologene: 223
Plg
Name: plasminogen
Synonyms: Pg
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 18815
VEGA: 17
HGNC: HGNC:9071
Homologene: 55452
Luc7l3
Name: LUC7-like 3 (S. cerevisiae)
Synonyms: 3300001P08Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 67684
Homologene: 75056
Kansl2
Name: KAT8 regulatory NSL complex subunit 2
Synonyms: 2310037I24Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 69612
VEGA: 15
Homologene: 32367
Mybl1
Name: myeloblastosis oncogene-like 1
Synonyms: A-myb, repro9, G1-419-6
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 17864
HGNC: HGNC:7547
Homologene: 32045
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 9,678,288 bp
  • T to A, chromosome 1 at 55,697,463 bp
  • T to C, chromosome 1 at 78,499,593 bp
  • T to A, chromosome 1 at 87,099,433 bp
  • C to T, chromosome 1 at 128,216,495 bp
  • A to G, chromosome 1 at 185,204,297 bp
  • G to A, chromosome 2 at 27,454,399 bp
  • A to G, chromosome 2 at 32,995,078 bp
  • A to C, chromosome 2 at 88,070,577 bp
  • T to A, chromosome 2 at 103,092,468 bp
  • A to T, chromosome 2 at 111,379,879 bp
  • A to C, chromosome 2 at 177,509,525 bp
  • T to C, chromosome 3 at 38,980,659 bp
  • T to A, chromosome 3 at 40,934,936 bp
  • G to A, chromosome 3 at 82,011,975 bp
  • G to T, chromosome 4 at 18,110,550 bp
  • G to A, chromosome 4 at 32,719,184 bp
  • A to G, chromosome 4 at 56,743,769 bp
  • A to T, chromosome 4 at 118,753,851 bp
  • T to A, chromosome 5 at 63,807,494 bp
  • C to A, chromosome 5 at 113,137,793 bp
  • A to G, chromosome 6 at 70,881,626 bp
  • C to T, chromosome 6 at 140,633,700 bp
  • T to G, chromosome 6 at 149,327,843 bp
  • A to G, chromosome 7 at 22,790,676 bp
  • A to G, chromosome 7 at 29,875,366 bp
  • A to G, chromosome 7 at 64,268,697 bp
  • T to A, chromosome 7 at 65,693,926 bp
  • A to T, chromosome 7 at 119,478,326 bp
  • T to C, chromosome 7 at 127,330,213 bp
  • C to T, chromosome 7 at 141,324,596 bp
  • A to G, chromosome 7 at 141,864,017 bp
  • A to G, chromosome 8 at 26,071,086 bp
  • A to G, chromosome 8 at 66,513,101 bp
  • T to A, chromosome 8 at 122,360,537 bp
  • A to T, chromosome 8 at 123,966,611 bp
  • T to C, chromosome 9 at 21,593,453 bp
  • G to T, chromosome 9 at 38,116,663 bp
  • A to T, chromosome 9 at 57,805,535 bp
  • T to C, chromosome 9 at 107,908,193 bp
  • A to T, chromosome 10 at 7,638,182 bp
  • T to A, chromosome 10 at 21,017,932 bp
  • C to T, chromosome 10 at 27,366,152 bp
  • G to T, chromosome 10 at 85,884,537 bp
  • T to A, chromosome 11 at 23,464,585 bp
  • T to A, chromosome 11 at 29,838,529 bp
  • T to G, chromosome 11 at 58,968,578 bp
  • T to A, chromosome 11 at 62,583,088 bp
  • T to C, chromosome 11 at 94,299,984 bp
  • A to T, chromosome 12 at 71,216,057 bp
  • A to G, chromosome 13 at 63,512,060 bp
  • T to C, chromosome 13 at 73,571,472 bp
  • G to T, chromosome 13 at 81,397,209 bp
  • A to G, chromosome 13 at 98,789,463 bp
  • A to G, chromosome 14 at 4,172,501 bp
  • C to T, chromosome 14 at 30,934,107 bp
  • A to T, chromosome 14 at 66,058,792 bp
  • G to T, chromosome 15 at 8,645,902 bp
  • G to A, chromosome 15 at 55,791,265 bp
  • T to C, chromosome 15 at 98,529,544 bp
  • A to G, chromosome 16 at 23,079,641 bp
  • A to G, chromosome 16 at 36,015,962 bp
  • G to A, chromosome 17 at 12,388,559 bp
  • A to C, chromosome 17 at 34,572,693 bp
  • T to A, chromosome 17 at 71,089,947 bp
  • G to T, chromosome 17 at 75,291,366 bp
  • T to A, chromosome 18 at 12,986,313 bp
  • A to C, chromosome 18 at 46,881,322 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7168 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045263-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.