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Strain Name:
C57BL/6J-MtgxR7191Btlr/Mmmh
Stock Number:
045274-MU
Citation ID:
RRID:MMRRC_045274-MU
Other Names:
R7191 (G1)
Major Collection:

Strain Information

Ank2
Name: ankyrin 2, brain
Synonyms: ankyrin B, Ank-2, Ankyrin-2, Ankyrin-B, Gm4392
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 109676
HGNC: HGNC:493
Gria2
Name: glutamate receptor, ionotropic, AMPA2 (alpha 2)
Synonyms: GluR-B, GluR2, Glur-2, Glur2, GluA2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 14800
HGNC: HGNC:4572
Homologene: 20225
Gcg
Name: glucagon
Synonyms: GLP-1, glucagon-like peptide I, Glu, PPG
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14526
HGNC: HGNC:4191
Homologene: 1553
Nrcam
Name: neuronal cell adhesion molecule
Synonyms: Bravo, C030017F07Rik, C130076O07Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 319504
VEGA: 12
HGNC: HGNC:7994
Homologene: 21041
Traip
Name: TRAF-interacting protein
Synonyms: Trip
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 22036
Homologene: 31343
Fryl
Name: FRY like transcription coactivator
Synonyms: 2310004H21Rik, 2510002A14Rik, 9030227G01Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 72313
Homologene: 103956
Cyp39a1
Name: cytochrome P450, family 39, subfamily a, polypeptide 1
Synonyms: oxysterol 7-alpha-hydroxylase
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 56050
VEGA: 17
Homologene: 9580
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 20,558,719 bp
  • A to G, chromosome 1 at 80,540,331 bp
  • T to A, chromosome 1 at 138,101,044 bp
  • A to C, chromosome 1 at 140,112,567 bp
  • G to A, chromosome 2 at 10,091,793 bp
  • C to G, chromosome 2 at 62,476,839 bp
  • A to T, chromosome 2 at 80,658,379 bp
  • A to T, chromosome 2 at 84,724,146 bp
  • C to T, chromosome 3 at 30,624,878 bp
  • C to A, chromosome 3 at 80,732,085 bp
  • T to C, chromosome 3 at 103,735,860 bp
  • T to A, chromosome 3 at 126,946,392 bp
  • T to C, chromosome 3 at 132,737,555 bp
  • T to C, chromosome 3 at 146,002,358 bp
  • T to C, chromosome 4 at 44,040,266 bp
  • A to G, chromosome 4 at 146,121,903 bp
  • T to C, chromosome 5 at 73,072,912 bp
  • A to G, chromosome 5 at 96,614,912 bp
  • T to C, chromosome 5 at 114,633,140 bp
  • T to C, chromosome 6 at 85,440,111 bp
  • T to C, chromosome 6 at 131,686,982 bp
  • T to A, chromosome 7 at 10,100,553 bp
  • T to C, chromosome 7 at 19,223,230 bp
  • C to T, chromosome 7 at 24,723,334 bp
  • G to A, chromosome 7 at 27,201,123 bp
  • C to A, chromosome 7 at 28,414,261 bp
  • T to C, chromosome 7 at 101,384,992 bp
  • G to A, chromosome 7 at 105,765,439 bp
  • G to A, chromosome 7 at 107,990,646 bp
  • A to T, chromosome 7 at 131,044,520 bp
  • G to A, chromosome 7 at 138,989,528 bp
  • C to T, chromosome 9 at 13,252,472 bp
  • A to G, chromosome 9 at 43,311,609 bp
  • T to G, chromosome 9 at 45,055,244 bp
  • A to G, chromosome 9 at 107,970,017 bp
  • A to G, chromosome 10 at 127,813,418 bp
  • C to T, chromosome 11 at 40,736,603 bp
  • T to C, chromosome 11 at 83,276,749 bp
  • T to C, chromosome 11 at 100,246,658 bp
  • C to T, chromosome 11 at 114,868,617 bp
  • T to C, chromosome 11 at 115,348,469 bp
  • C to T, chromosome 11 at 118,268,834 bp
  • A to T, chromosome 12 at 40,329,562 bp
  • A to G, chromosome 12 at 44,572,244 bp
  • A to G, chromosome 12 at 57,457,066 bp
  • A to G, chromosome 13 at 23,981,493 bp
  • C to T, chromosome 15 at 76,003,037 bp
  • T to C, chromosome 15 at 101,460,229 bp
  • T to A, chromosome 16 at 21,653,564 bp
  • A to G, chromosome 16 at 96,151,945 bp
  • A to G, chromosome 17 at 18,930,286 bp
  • G to A, chromosome 17 at 43,731,019 bp
  • A to T, chromosome 18 at 60,246,257 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7191 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045274-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.