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Strain Name:
C57BL/6J-MtgxR7214Btlr/Mmmh
Stock Number:
045286-MU
Citation ID:
RRID:MMRRC_045286-MU
Other Names:
R7214 (G1)
Major Collection:

Strain Information

Nfat5
Name: nuclear factor of activated T cells 5
Synonyms: nfatz, TonEBP, B130038B15Rik, OREBP
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 54446
HGNC: HGNC:7774
Homologene: 4811
Ptprk
Name: protein tyrosine phosphatase receptor type K
Synonyms: PTPk, RPTPkappa
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 19272
VEGA: 10
HGNC: HGNC:9674
Homologene: 55693
Cd2ap
Name: CD2-associated protein
Synonyms: METS-1, Mets1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 12488
VEGA: 17
Homologene: 7663
Crlf3
Name: cytokine receptor-like factor 3
Synonyms: cytor4, Creme9
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 54394
Homologene: 9327
Dop1b
Name: DOP1 leucine zipper like protein B
Synonyms: 0610038M01Rik, 2610510B01Rik, Dopey2
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 70028
VEGA: 16
HGNC: HGNC:1291
Homologene: 21068
Prpf6
Name: pre-mRNA splicing factor 6
Synonyms: 1190003A07Rik, U5-102K, ANT-1, 2610031L17Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 68879
Homologene: 5368
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 54,543,061 bp
  • A to T, chromosome 1 at 59,562,729 bp
  • G to C, chromosome 1 at 60,237,151 bp
  • T to A, chromosome 1 at 80,568,529 bp
  • C to A, chromosome 1 at 128,300,460 bp
  • A to T, chromosome 1 at 131,759,473 bp
  • A to T, chromosome 1 at 151,394,613 bp
  • T to C, chromosome 2 at 29,227,720 bp
  • C to T, chromosome 2 at 30,172,890 bp
  • T to A, chromosome 2 at 30,307,554 bp
  • T to C, chromosome 2 at 36,688,095 bp
  • C to T, chromosome 2 at 86,525,806 bp
  • T to C, chromosome 2 at 101,729,432 bp
  • T to A, chromosome 2 at 121,169,280 bp
  • T to A, chromosome 2 at 137,046,730 bp
  • T to C, chromosome 2 at 137,106,882 bp
  • T to C, chromosome 2 at 150,409,281 bp
  • T to C, chromosome 2 at 152,687,377 bp
  • C to T, chromosome 2 at 181,640,596 bp
  • G to A, chromosome 3 at 36,650,137 bp
  • A to T, chromosome 3 at 75,358,444 bp
  • G to C, chromosome 3 at 86,328,326 bp
  • G to T, chromosome 3 at 130,033,860 bp
  • T to A, chromosome 4 at 118,933,949 bp
  • C to T, chromosome 5 at 31,052,246 bp
  • T to C, chromosome 5 at 31,503,417 bp
  • C to T, chromosome 5 at 62,749,338 bp
  • A to T, chromosome 5 at 65,131,755 bp
  • A to C, chromosome 5 at 90,786,360 bp
  • A to G, chromosome 5 at 104,522,363 bp
  • G to A, chromosome 5 at 110,589,828 bp
  • G to A, chromosome 5 at 122,313,777 bp
  • T to C, chromosome 5 at 140,360,253 bp
  • T to C, chromosome 6 at 91,492,338 bp
  • T to A, chromosome 6 at 126,885,237 bp
  • T to A, chromosome 6 at 136,612,831 bp
  • C to T, chromosome 7 at 8,253,380 bp
  • T to C, chromosome 7 at 26,551,038 bp
  • T to C, chromosome 7 at 48,830,637 bp
  • CGGCGGC to CGGCGGCGGGGGCGGC, chromosome 7 at 97,579,929 bp
  • T to C, chromosome 7 at 106,874,267 bp
  • T to C, chromosome 7 at 107,098,412 bp
  • T to A, chromosome 7 at 113,299,403 bp
  • G to A, chromosome 7 at 119,922,742 bp
  • C to T, chromosome 7 at 126,582,770 bp
  • A to G, chromosome 7 at 127,391,523 bp
  • A to T, chromosome 7 at 127,405,278 bp
  • A to T, chromosome 8 at 21,963,625 bp
  • T to C, chromosome 8 at 91,083,429 bp
  • C to T, chromosome 8 at 94,934,018 bp
  • T to C, chromosome 8 at 107,293,883 bp
  • A to T, chromosome 8 at 108,948,861 bp
  • T to G, chromosome 8 at 110,671,042 bp
  • T to A, chromosome 8 at 117,583,549 bp
  • T to A, chromosome 8 at 122,568,273 bp
  • A to C, chromosome 9 at 38,180,022 bp
  • A to T, chromosome 9 at 45,670,139 bp
  • A to G, chromosome 9 at 50,597,233 bp
  • G to T, chromosome 9 at 56,937,723 bp
  • T to C, chromosome 10 at 4,410,753 bp
  • T to C, chromosome 10 at 28,574,909 bp
  • C to T, chromosome 10 at 76,547,471 bp
  • C to A, chromosome 10 at 86,899,841 bp
  • C to T, chromosome 10 at 88,379,157 bp
  • G to A, chromosome 10 at 88,919,502 bp
  • T to C, chromosome 11 at 6,511,055 bp
  • C to T, chromosome 11 at 22,990,616 bp
  • T to C, chromosome 11 at 29,137,285 bp
  • T to A, chromosome 11 at 54,411,912 bp
  • T to C, chromosome 11 at 69,431,109 bp
  • C to T, chromosome 11 at 71,123,293 bp
  • T to C, chromosome 11 at 80,064,390 bp
  • C to T, chromosome 11 at 83,589,756 bp
  • C to T, chromosome 13 at 33,664,145 bp
  • T to C, chromosome 13 at 68,589,380 bp
  • T to C, chromosome 13 at 106,895,857 bp
  • T to C, chromosome 14 at 55,498,612 bp
  • T to A, chromosome 14 at 61,191,792 bp
  • A to G, chromosome 14 at 79,213,310 bp
  • T to A, chromosome 15 at 9,108,254 bp
  • C to T, chromosome 15 at 27,871,187 bp
  • T to C, chromosome 15 at 74,836,859 bp
  • T to C, chromosome 15 at 99,470,463 bp
  • A to G, chromosome 16 at 3,988,980 bp
  • T to A, chromosome 16 at 4,701,182 bp
  • T to G, chromosome 16 at 93,810,135 bp
  • T to A, chromosome 17 at 3,518,412 bp
  • T to C, chromosome 17 at 25,845,393 bp
  • C to T, chromosome 17 at 42,845,394 bp
  • T to G, chromosome 17 at 45,661,822 bp
  • T to C, chromosome 17 at 71,345,364 bp
  • C to A, chromosome 18 at 37,690,106 bp
  • T to C, chromosome 19 at 5,760,029 bp
  • T to A, chromosome 19 at 13,470,973 bp
  • C to A, chromosome 19 at 56,378,135 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7214 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045286-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.