Strain Name:
C57BL/6J-MtgxR7227Btlr/Mmmh
Stock Number:
045299-MU
Citation ID:
RRID:MMRRC_045299-MU
Other Names:
R7227 (G1)
Major Collection:

Strain Information

Xrcc1
Name: X-ray repair complementing defective repair in Chinese hamster cells 1
Synonyms: Xrcc-1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 22594
Homologene: 31368
Wrn
Name: Werner syndrome RecQ like helicase
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 22427
Homologene: 6659
Nucb2
Name: nucleobindin 2
Synonyms: NEFA, Calnuc, nesfatin-1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 53322
HGNC: HGNC:8044
Homologene: 3676
Cxcl17
Name: C-X-C motif chemokine ligand 17
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 232983
Homologene: 18875
Mef2d
Name: myocyte enhancer factor 2D
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 17261
HGNC: HGNC:6997
Homologene: 4327
Pdk1
Name: pyruvate dehydrogenase kinase, isoenzyme 1
Synonyms: D530020C15Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228026
HGNC: HGNC:8809
Homologene: 134437
Nfkb1
Name: nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105
Synonyms: NF kappaB1, p50 subunit of NF kappaB, p50/p105, p50, nuclear factor kappaB p50, NF-kappaB, NF-kappaB p50
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18033
HGNC: HGNC:7794
Homologene: 2971
Thrap3
Name: thyroid hormone receptor associated protein 3
Synonyms: 9330151F09Rik, Trap150, B230333E16Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230753
Homologene: 31289
Ap2a2
Name: adaptor-related protein complex 2, alpha 2 subunit
Synonyms: alpha-C adaptin, alpha-adaptin C, L25, Adtab, 2410074K14Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 11772
HGNC: HGNC:562
Homologene: 5335
Gigyf1
Name: GRB10 interacting GYF protein 1
Synonyms: Perq1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 57330
HGNC: HGNC:9126
Homologene: 57145
Sec16a
Name: SEC16 homolog A, endoplasmic reticulum export factor
Synonyms: C230052J16Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227648
Homologene: 10533
Esyt2
Name: extended synaptotagmin-like protein 2
Synonyms: 2410017M09Rik, 4921504I16Rik, 2310058N22Rik, D12Ertd551e, Fam62b
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 52635
VEGA: 12
Homologene: 32699
Bend3
Name: BEN domain containing 3
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 331623
VEGA: 10
Homologene: 19511
2310057M21Rik
Name: RIKEN cDNA 2310057M21 gene
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 68277
Homologene: 11793
Ascc1
Name: activating signal cointegrator 1 complex subunit 1
Synonyms: ASC1p50, CGI-18, 1810015P09Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 69090
VEGA: 10
Homologene: 41079
Slc8a2
Name: solute carrier family 8 (sodium/calcium exchanger), member 2
Synonyms: Ncx2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 110891
Homologene: 27358
Orc3
Name: origin recognition complex, subunit 3
Synonyms: Orc3l
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 50793
HGNC: HGNC:8489
Homologene: 8225
Mybbp1a
Name: MYB binding protein (P160) 1a
Synonyms: p67MBP, p160MBP
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18432
HGNC: HGNC:7546
Homologene: 40954
Dzip3
Name: DAZ interacting protein 3, zinc finger
Synonyms: 6430549P11Rik, 2310047C04Rik, 2A-HUB
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 224170
Homologene: 8771
Enpp3
Name: ectonucleotide pyrophosphatase/phosphodiesterase 3
Synonyms: CD203c
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 209558
VEGA: 10
HGNC: HGNC:3358
Homologene: 3683
Ube2d1
Name: ubiquitin-conjugating enzyme E2D 1
Synonyms: UBCH5
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 216080
Homologene: 20714
Mpp3
Name: membrane protein, palmitoylated 3 (MAGUK p55 subfamily member 3)
Synonyms: Dlgh3
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 13384
HGNC: HGNC:7221
Homologene: 31065
Fat1
Name: FAT atypical cadherin 1
Synonyms: mFat1, 2310038E12Rik, Fath
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 14107
HGNC: HGNC:3595
Homologene: 66302
Stk40
Name: serine/threonine kinase 40
Synonyms: 2310004N11Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 74178
Homologene: 12542
Ppan
Name: peter pan homolog
Synonyms: SSF1, A230087P06Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235036
Homologene: 5690
Rap1gds1
Name: RAP1, GTP-GDP dissociation stimulator 1
Synonyms: GDS1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229877
HGNC: HGNC:9859
Homologene: 41422
Slc44a3
Name: solute carrier family 44, member 3
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 213603
Homologene: 64830
Gja1
Name: gap junction protein, alpha 1
Synonyms: connexin 43, Cx43, alpha 1 connexin, connexin43, Gja-1, Cx43alpha1, Cnx43
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 14609
VEGA: 10
HGNC: HGNC:4274
Homologene: 136
Ier3ip1
Name: immediate early response 3 interacting protein 1
Synonyms: 1110057H19Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 66191
Homologene: 41106
Cfap74
Name: cilia and flagella associated protein 74
Synonyms: 2010015L04Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 544678
Homologene: 129584
Dnah2
Name: dynein, axonemal, heavy chain 2
Synonyms: D330014H01Rik, 2900022L05Rik, Dnhd3, Dnahc2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 327954
HGNC: HGNC:2948
Homologene: 72110
Mab21l4
Name: mab-21-like 4
Synonyms: 2310007B03Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 71874
Homologene: 11761
Scn2a
Name: sodium channel, voltage-gated, type II, alpha
Synonyms: Nav1.2, A230052E19Rik, Scn2a1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 110876
Homologene: 75001
Vmn1r67
Name: vomeronasal 1 receptor 67
Synonyms: V1re10
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 171263
Homologene: 128344
Abcb1b
Name: ATP-binding cassette, sub-family B member 1B
Synonyms: Mdr1, Mdr1b, mdr, Pgy1, Pgy-1, Abcb1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 18669
HGNC: HGNC:40
Homologene: 69084
Kremen2
Name: kringle containing transmembrane protein 2
Synonyms: 2900054E04Rik, Krm2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 73016
VEGA: 17
Homologene: 65132
Plce1
Name: phospholipase C, epsilon 1
Synonyms: 4933403A21Rik, PLCepsilon
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 74055
Homologene: 9478
Klhl5
Name: kelch-like 5
Synonyms: 1300013C10Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 71778
HGNC: HGNC:6356
Homologene: 56736
Hivep1
Name: human immunodeficiency virus type I enhancer binding protein 1
Synonyms: Cryabp1, alphaA-CRYBP1
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 110521
VEGA: 13
HGNC: HGNC:4920
Homologene: 1596
Tmc5
Name: transmembrane channel-like gene family 5
Synonyms: 4932443L08Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 74424
Homologene: 11713
Mrpl12
Name: mitochondrial ribosomal protein L12
Synonyms: MRP-L12, Rpml12, 1500031N16Rik, 0610034O11Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 56282
Homologene: 2212
Vmn2r18
Name: vomeronasal 2, receptor 18
Synonyms: EG632671
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 632671
Zkscan4
Name: zinc finger with KRAB and SCAN domains 4
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 544922
Homologene: 128718
Ssbp2
Name: single-stranded DNA binding protein 2
Synonyms: Hspc116, 2310079I02Rik, 9330163K02Rik, 1500004K09Rik, A830008M03Rik, Ssdp2
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 66970
Homologene: 22724
Smco1
Name: single-pass membrane protein with coiled-coil domains 1
Synonyms: 2310010M20Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 69576
Homologene: 18842
Serpinb3a
Name: serine (or cysteine) peptidase inhibitor, clade B (ovalbumin), member 3A
Synonyms: Sqn5
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 20248
Homologene: 76659
Acsm2
Name: acyl-CoA synthetase medium-chain family member 2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233799
Homologene: 70404
Hk3
Name: hexokinase 3
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 212032
HGNC: HGNC:4925
Homologene: 55633
Slc30a8
Name: solute carrier family 30 (zinc transporter), member 8
Synonyms: ZnT-8, C820002P14Rik, ZnT8
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 239436
Homologene: 13795
Cyp2c69
Name: cytochrome P450, family 2, subfamily c, polypeptide 69
Synonyms: AI098658
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 100043108
VEGA: 19
Homologene: 74936
Mkrn3
Name: makorin, ring finger protein, 3
Synonyms: D7H15S9-1, Zfp127
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 22652
HGNC: HGNC:7114
Homologene: 4143
Trim38
Name: tripartite motif-containing 38
Synonyms: LOC214158
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 214158
Homologene: 48427
Map4k2
Name: mitogen-activated protein kinase kinase kinase kinase 2
Synonyms: BL44, Rab8ip
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 26412
HGNC: HGNC:6864
Homologene: 3370
Spns2
Name: SPNS lysolipid transporter 2, sphingosine-1-phosphate
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 216892
Homologene: 71040
Or12j2
Name: olfactory receptor family 12 subfamily J member 2
Synonyms: GA_x6K02T2PBJ9-42486061-42486978, MOR251-5, Olfr527
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 257939
Homologene: 73958
Lag3
Name: lymphocyte-activation gene 3
Synonyms: CD223, Ly66, LAG-3
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 16768
HGNC: HGNC:6476
Homologene: 1719
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 93,152,014 bp
  • T to A, chromosome 1 at 107,051,629 bp
  • A to T, chromosome 2 at 26,438,923 bp
  • A to C, chromosome 2 at 65,752,023 bp
  • T to A, chromosome 2 at 71,883,901 bp
  • G to A, chromosome 3 at 88,158,207 bp
  • A to T, chromosome 3 at 121,510,230 bp
  • C to T, chromosome 3 at 135,626,659 bp
  • C to T, chromosome 3 at 138,957,467 bp
  • T to C, chromosome 4 at 34,572,542 bp
  • G to T, chromosome 4 at 126,123,766 bp
  • A to T, chromosome 4 at 126,173,503 bp
  • T to G, chromosome 4 at 155,460,948 bp
  • T to A, chromosome 5 at 8,825,593 bp
  • T to C, chromosome 5 at 65,141,288 bp
  • T to A, chromosome 5 at 137,523,823 bp
  • T to C, chromosome 5 at 151,572,799 bp
  • C to T, chromosome 6 at 124,908,494 bp
  • T to A, chromosome 7 at 10,447,548 bp
  • T to C, chromosome 7 at 16,144,981 bp
  • C to A, chromosome 7 at 24,547,332 bp
  • C to A, chromosome 7 at 25,402,894 bp
  • C to A, chromosome 7 at 62,419,667 bp
  • A to T, chromosome 7 at 116,526,076 bp
  • A to G, chromosome 7 at 118,670,666 bp
  • A to C, chromosome 7 at 119,591,333 bp
  • A to T, chromosome 7 at 131,350,720 bp
  • A to G, chromosome 7 at 140,335,621 bp
  • T to C, chromosome 7 at 141,620,871 bp
  • T to A, chromosome 8 at 33,248,946 bp
  • T to G, chromosome 8 at 45,010,609 bp
  • T to A, chromosome 9 at 20,888,200 bp
  • C to T, chromosome 10 at 24,817,844 bp
  • T to A, chromosome 10 at 43,511,405 bp
  • T to A, chromosome 10 at 56,387,656 bp
  • T to A, chromosome 10 at 60,007,738 bp
  • T to A, chromosome 10 at 71,255,872 bp
  • A to G, chromosome 11 at 69,421,396 bp
  • G to T, chromosome 11 at 72,447,759 bp
  • A to T, chromosome 11 at 72,458,687 bp
  • T to A, chromosome 11 at 102,005,078 bp
  • T to A, chromosome 11 at 120,488,352 bp
  • A to G, chromosome 12 at 116,342,125 bp
  • T to C, chromosome 13 at 21,484,243 bp
  • T to A, chromosome 13 at 23,785,963 bp
  • T to C, chromosome 13 at 42,156,911 bp
  • C to T, chromosome 13 at 55,012,240 bp
  • T to C, chromosome 13 at 91,675,125 bp
  • A to G, chromosome 15 at 52,331,636 bp
  • T to C, chromosome 16 at 32,274,015 bp
  • T to A, chromosome 16 at 48,951,569 bp
  • G to T, chromosome 17 at 23,744,599 bp
  • T to A, chromosome 18 at 76,939,634 bp
  • T to A, chromosome 19 at 6,346,594 bp
  • A to G, chromosome 19 at 38,726,902 bp
  • C to A, chromosome 19 at 39,881,166 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7227 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045299-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.