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Strain Name:
C57BL/6J-MtgxR7251Btlr/Mmmh
Stock Number:
045313-MU
Citation ID:
RRID:MMRRC_045313-MU
Other Names:
R7251 (G1)
Major Collection:

Strain Information

Hgf
Name: hepatocyte growth factor
Synonyms: scatter factor, NK1, SF/HGF, HGF/SF, NK2, C230052L06Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 15234
HGNC: HGNC:4893
Homologene: 503
Pmpca
Name: peptidase (mitochondrial processing) alpha
Synonyms: INPP5E, Alpha-MPP, 4933435E07Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 66865
Homologene: 6078
Mgll
Name: monoglyceride lipase
Synonyms: Magl
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 23945
Homologene: 38298
Stk24
Name: serine/threonine kinase 24
Synonyms: 1810013H02Rik, STE20
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 223255
VEGA: 14
Homologene: 20793
Agrn
Name: agrin
Synonyms: NMF380, Agrin, nmf380
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11603
HGNC: HGNC:329
Homologene: 27907
Ptpn2
Name: protein tyrosine phosphatase, non-receptor type 2
Synonyms: Ptpt, TC-PTP
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 19255
HGNC: HGNC:9650
Homologene: 7497
Slain2
Name: SLAIN motif family, member 2
Synonyms: 8030444K12Rik, 5033405K12Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 75991
Homologene: 18952
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 10,198,975 bp
  • C to T, chromosome 1 at 13,148,375 bp
  • A to G, chromosome 1 at 60,489,868 bp
  • T to C, chromosome 2 at 25,938,886 bp
  • G to C, chromosome 2 at 26,395,034 bp
  • G to A, chromosome 2 at 82,979,081 bp
  • G to A, chromosome 2 at 86,423,596 bp
  • A to G, chromosome 2 at 90,700,174 bp
  • A to T, chromosome 2 at 91,162,176 bp
  • T to A, chromosome 2 at 104,221,166 bp
  • T to C, chromosome 2 at 119,332,292 bp
  • T to A, chromosome 2 at 121,347,571 bp
  • T to A, chromosome 2 at 158,028,979 bp
  • C to T, chromosome 2 at 181,689,748 bp
  • T to A, chromosome 3 at 59,319,454 bp
  • T to C, chromosome 3 at 94,776,199 bp
  • T to C, chromosome 3 at 152,247,195 bp
  • C to T, chromosome 4 at 134,580,836 bp
  • T to C, chromosome 4 at 156,174,606 bp
  • T to A, chromosome 5 at 16,593,944 bp
  • C to G, chromosome 5 at 67,427,533 bp
  • T to A, chromosome 5 at 72,974,548 bp
  • T to A, chromosome 5 at 110,127,960 bp
  • T to C, chromosome 5 at 123,994,750 bp
  • T to C, chromosome 6 at 25,752,498 bp
  • A to G, chromosome 6 at 39,677,570 bp
  • A to G, chromosome 6 at 48,064,877 bp
  • A to G, chromosome 6 at 52,721,356 bp
  • A to G, chromosome 6 at 83,135,962 bp
  • A to G, chromosome 6 at 83,404,056 bp
  • A to G, chromosome 6 at 88,823,375 bp
  • A to T, chromosome 6 at 125,226,595 bp
  • G to A, chromosome 7 at 27,157,719 bp
  • T to C, chromosome 7 at 86,413,001 bp
  • C to T, chromosome 7 at 103,639,702 bp
  • A to G, chromosome 7 at 141,692,722 bp
  • T to A, chromosome 9 at 22,108,217 bp
  • T to C, chromosome 9 at 32,208,185 bp
  • T to A, chromosome 9 at 42,387,752 bp
  • A to T, chromosome 9 at 121,785,050 bp
  • A to T, chromosome 10 at 81,210,532 bp
  • T to C, chromosome 10 at 127,572,554 bp
  • A to G, chromosome 11 at 32,731,370 bp
  • A to T, chromosome 11 at 58,341,302 bp
  • C to A, chromosome 11 at 101,265,153 bp
  • T to C, chromosome 12 at 8,007,037 bp
  • T to C, chromosome 12 at 17,402,107 bp
  • T to C, chromosome 12 at 37,981,986 bp
  • G to A, chromosome 12 at 79,150,798 bp
  • TC to T, chromosome 12 at 104,961,571 bp
  • T to A, chromosome 13 at 4,447,020 bp
  • A to G, chromosome 13 at 38,193,548 bp
  • G to T, chromosome 13 at 73,992,097 bp
  • A to G, chromosome 14 at 14,853,965 bp
  • T to C, chromosome 14 at 34,146,915 bp
  • A to T, chromosome 14 at 121,308,022 bp
  • C to T, chromosome 15 at 85,809,293 bp
  • A to G, chromosome 16 at 20,315,896 bp
  • A to G, chromosome 16 at 88,829,094 bp
  • A to G, chromosome 16 at 97,743,249 bp
  • A to G, chromosome 17 at 35,253,488 bp
  • G to A, chromosome 18 at 10,515,319 bp
  • A to T, chromosome 18 at 58,837,902 bp
  • A to C, chromosome 18 at 67,675,792 bp
  • T to A, chromosome 18 at 84,622,197 bp
  • A to T, chromosome 19 at 59,300,645 bp
  • G to T, chromosome X at 78,370,705 bp
  • A to G, chromosome Y at 1,154,262 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7251 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045313-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.