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Strain Name:
C57BL/6J-MtgxR7257Btlr/Mmmh
Stock Number:
045318-MU
Citation ID:
RRID:MMRRC_045318-MU
Other Names:
R7257 (G1)
Major Collection:

Strain Information

Phb1
Name: prohibitin 1
Synonyms: Phb
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18673
HGNC: HGNC:8912
Homologene: 1980
Myh7
Name: myosin, heavy polypeptide 7, cardiac muscle, beta
Synonyms: Myhc-b, Myhcb, beta-MHC, B-MHC, MYH-beta/slow, MyHC-I, betaMHC
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 140781
HGNC: HGNC:7577
Homologene: 68044
Septin3
Name: septin 3
Synonyms: Sep3, B530002E20Rik, 3110018K01Rik, Gm46500, Sept3
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 24050
VEGA: 15
Homologene: 99740
Ptch1
Name: patched 1
Synonyms: Ptc, Patched 1, Ptc1, A230106A15Rik, wig
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 19206
HGNC: HGNC:9585
Homologene: 223
Adk
Name: adenosine kinase
Synonyms: AK, 5033405D03Rik, 2310026J05Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 11534
VEGA: 14
HGNC: HGNC:257
Homologene: 4891
Rapgef2
Name: Rap guanine nucleotide exchange factor (GEF) 2
Synonyms: 5830453M24Rik, Pdzgef1, RA-GEF-1, CNRasGEF, nRapGEP
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 76089
Homologene: 35477
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 161,032,661 bp
  • T to A, chromosome 1 at 182,974,233 bp
  • A to T, chromosome 2 at 25,245,839 bp
  • A to T, chromosome 2 at 27,067,368 bp
  • T to C, chromosome 2 at 32,580,431 bp
  • A to T, chromosome 2 at 60,427,625 bp
  • T to A, chromosome 2 at 71,249,356 bp
  • A to G, chromosome 2 at 76,741,094 bp
  • A to G, chromosome 2 at 87,943,571 bp
  • A to T, chromosome 2 at 92,412,981 bp
  • A to G, chromosome 3 at 27,138,535 bp
  • C to A, chromosome 3 at 66,158,282 bp
  • A to T, chromosome 3 at 79,082,627 bp
  • T to A, chromosome 4 at 98,973,412 bp
  • G to A, chromosome 4 at 106,758,402 bp
  • C to A, chromosome 4 at 140,829,471 bp
  • A to C, chromosome 5 at 87,887,545 bp
  • T to G, chromosome 5 at 121,785,817 bp
  • T to A, chromosome 5 at 124,125,356 bp
  • T to A, chromosome 5 at 124,560,721 bp
  • C to A, chromosome 5 at 129,595,633 bp
  • A to T, chromosome 6 at 88,839,452 bp
  • A to G, chromosome 6 at 110,646,118 bp
  • A to G, chromosome 6 at 116,107,338 bp
  • G to A, chromosome 7 at 13,325,744 bp
  • A to T, chromosome 7 at 21,272,825 bp
  • T to A, chromosome 7 at 31,035,151 bp
  • G to A, chromosome 7 at 56,279,538 bp
  • T to A, chromosome 7 at 86,694,804 bp
  • T to C, chromosome 7 at 103,051,630 bp
  • C to T, chromosome 7 at 107,794,433 bp
  • T to A, chromosome 7 at 109,460,244 bp
  • A to G, chromosome 7 at 133,759,477 bp
  • T to A, chromosome 8 at 54,632,487 bp
  • G to T, chromosome 8 at 109,940,188 bp
  • A to T, chromosome 9 at 35,189,034 bp
  • T to A, chromosome 9 at 70,543,965 bp
  • C to T, chromosome 9 at 72,452,815 bp
  • T to A, chromosome 9 at 108,069,029 bp
  • C to A, chromosome 10 at 80,806,653 bp
  • G to A, chromosome 10 at 121,413,019 bp
  • A to G, chromosome 10 at 128,944,413 bp
  • C to T, chromosome 10 at 129,046,455 bp
  • T to A, chromosome 10 at 129,912,287 bp
  • A to G, chromosome 11 at 49,626,009 bp
  • T to A, chromosome 11 at 61,684,753 bp
  • A to G, chromosome 11 at 67,181,150 bp
  • A to T, chromosome 11 at 68,992,959 bp
  • T to A, chromosome 11 at 77,137,779 bp
  • A to T, chromosome 11 at 95,678,091 bp
  • T to C, chromosome 12 at 69,202,910 bp
  • T to C, chromosome 12 at 69,741,669 bp
  • A to G, chromosome 12 at 100,842,738 bp
  • A to T, chromosome 12 at 105,164,531 bp
  • T to A, chromosome 13 at 4,088,966 bp
  • A to G, chromosome 13 at 21,133,257 bp
  • T to C, chromosome 13 at 63,573,294 bp
  • T to G, chromosome 13 at 76,056,466 bp
  • T to C, chromosome 14 at 20,747,839 bp
  • G to T, chromosome 14 at 21,052,671 bp
  • T to G, chromosome 14 at 32,177,369 bp
  • T to G, chromosome 14 at 34,065,849 bp
  • T to A, chromosome 14 at 47,500,872 bp
  • A to T, chromosome 14 at 54,972,490 bp
  • G to A, chromosome 15 at 82,289,213 bp
  • G to A, chromosome 16 at 91,926,867 bp
  • T to A, chromosome 17 at 27,118,561 bp
  • A to G, chromosome 17 at 34,113,822 bp
  • A to G, chromosome 17 at 34,716,501 bp
  • A to G, chromosome 17 at 37,023,127 bp
  • T to A, chromosome 18 at 21,008,693 bp
  • A to T, chromosome 18 at 36,679,616 bp
  • A to G, chromosome 18 at 37,687,398 bp
  • T to A, chromosome 18 at 86,716,734 bp
  • A to G, chromosome 19 at 3,266,405 bp
  • T to A, chromosome 19 at 8,925,738 bp
  • T to A, chromosome 19 at 25,127,085 bp
  • C to T, chromosome 19 at 26,654,464 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7257 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045318-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.