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Strain Name:
C57BL/6J-MtgxR7265Btlr/Mmmh
Stock Number:
045355-MU
Citation ID:
RRID:MMRRC_045355-MU
Other Names:
R7265 (G1)
Major Collection:

Strain Information

Sez6
Name: seizure related gene 6
Synonyms: sez-6, D11Bhm177e
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20370
Homologene: 10948
Jarid2
Name: jumonji and AT-rich interaction domain containing 2
Synonyms: Jmj, jumonji
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 16468
HGNC: HGNC:6196
Homologene: 31279
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11426
Homologene: 136191
Tlk2
Name: tousled-like kinase 2 (Arabidopsis)
Synonyms: protein kinase U-alpha, PKUalpha, 4933403M19Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 24086
Homologene: 4993
Enpp2
Name: ectonucleotide pyrophosphatase/phosphodiesterase 2
Synonyms: Autotaxin, Npps2, PD-Ialpha, Pdnp2, ATX
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 18606
HGNC: HGNC:3357
Homologene: 4526
Pik3c2a
Name: phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 alpha
Synonyms: PI3KC2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 18704
HGNC: HGNC:8971
Homologene: 20581
Kif16b
Name: kinesin family member 16B
Synonyms: N-3 kinesin, 8430434E15Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 16558
Homologene: 135708
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to G, chromosome 1 at 96,871,793 bp
  • G to A, chromosome 1 at 120,022,225 bp
  • C to A, chromosome 1 at 155,225,327 bp
  • A to G, chromosome 2 at 21,212,458 bp
  • T to C, chromosome 2 at 66,210,173 bp
  • C to T, chromosome 2 at 86,355,744 bp
  • G to A, chromosome 2 at 136,101,185 bp
  • A to T, chromosome 2 at 142,714,730 bp
  • T to A, chromosome 2 at 152,004,416 bp
  • C to A, chromosome 3 at 29,980,133 bp
  • T to C, chromosome 3 at 64,556,774 bp
  • A to T, chromosome 3 at 90,403,983 bp
  • T to A, chromosome 3 at 106,128,923 bp
  • T to C, chromosome 3 at 137,417,078 bp
  • T to A, chromosome 3 at 137,419,076 bp
  • A to T, chromosome 4 at 123,407,877 bp
  • T to C, chromosome 4 at 131,967,834 bp
  • T to A, chromosome 4 at 139,669,652 bp
  • C to A, chromosome 4 at 143,895,421 bp
  • T to C, chromosome 5 at 9,446,975 bp
  • A to T, chromosome 5 at 33,778,648 bp
  • A to G, chromosome 5 at 34,716,264 bp
  • A to T, chromosome 5 at 35,564,303 bp
  • A to G, chromosome 5 at 36,476,608 bp
  • A to G, chromosome 5 at 100,660,270 bp
  • G to A, chromosome 5 at 112,812,072 bp
  • A to G, chromosome 5 at 149,709,320 bp
  • A to T, chromosome 6 at 113,735,136 bp
  • A to G, chromosome 6 at 149,513,876 bp
  • T to C, chromosome 7 at 19,451,725 bp
  • A to T, chromosome 7 at 35,487,722 bp
  • T to C, chromosome 7 at 98,499,437 bp
  • G to T, chromosome 7 at 105,384,225 bp
  • T to A, chromosome 7 at 116,388,086 bp
  • G to T, chromosome 7 at 119,466,073 bp
  • A to G, chromosome 8 at 72,692,928 bp
  • A to G, chromosome 9 at 18,656,472 bp
  • A to T, chromosome 9 at 38,108,931 bp
  • T to A, chromosome 9 at 64,126,921 bp
  • T to C, chromosome 9 at 95,708,275 bp
  • C to A, chromosome 9 at 119,815,265 bp
  • T to C, chromosome 10 at 75,721,423 bp
  • T to C, chromosome 11 at 6,510,933 bp
  • T to A, chromosome 11 at 8,929,402 bp
  • A to G, chromosome 11 at 30,998,495 bp
  • T to C, chromosome 11 at 75,669,790 bp
  • T to A, chromosome 11 at 77,962,865 bp
  • T to A, chromosome 11 at 103,498,941 bp
  • C to T, chromosome 11 at 105,184,244 bp
  • T to C, chromosome 12 at 38,184,932 bp
  • A to T, chromosome 12 at 101,053,511 bp
  • C to T, chromosome 12 at 103,400,221 bp
  • G to A, chromosome 13 at 44,902,272 bp
  • A to G, chromosome 14 at 33,614,524 bp
  • G to A, chromosome 14 at 51,418,319 bp
  • A to G, chromosome 14 at 103,197,243 bp
  • A to C, chromosome 15 at 54,910,033 bp
  • T to A, chromosome 16 at 59,283,924 bp
  • T to A, chromosome 17 at 3,177,625 bp
  • G to A, chromosome 17 at 33,066,997 bp
  • C to A, chromosome 17 at 33,631,279 bp
  • C to T, chromosome 17 at 34,475,894 bp
  • C to T, chromosome 17 at 65,929,787 bp
  • T to C, chromosome 18 at 36,739,343 bp
  • A to T, chromosome 18 at 37,505,563 bp
  • A to G, chromosome 18 at 37,716,916 bp
  • A to G, chromosome 19 at 4,923,769 bp
  • A to T, chromosome 19 at 29,321,545 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7265 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045355-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.