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Strain Name:
C57BL/6J-MtgxR7295Btlr/Mmmh
Stock Number:
045363-MU
Citation ID:
RRID:MMRRC_045363-MU
Other Names:
R7295 (G1)
Major Collection:

Strain Information

a
Name: nonagouti
Synonyms: agouti, As, agouti signal protein, ASP, Asip
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 50518
HGNC: HGNC:745
Homologene: 1264
Bmp7
Name: bone morphogenetic protein 7
Synonyms: osteogenic protein 1, OP1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 12162
HGNC: HGNC:1074
Homologene: 20410
Ntsr1
Name: neurotensin receptor 1
Synonyms: NT-1R, Ntsr1, NTR-1, NTR1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 18216
HGNC: HGNC:8039
Homologene: 68261
Kcnj5
Name: potassium inwardly-rectifying channel, subfamily J, member 5
Synonyms: Kir3.4, GIRK4
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 16521
VEGA: 9
HGNC: HGNC:6266
Homologene: 20248
Foxj2
Name: forkhead box J2
Synonyms: Fhx
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 60611
Homologene: 10187
Xpnpep3
Name: X-prolyl aminopeptidase 3, mitochondrial
Synonyms: E430012M05Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 321003
Eprs1
Name: glutamyl-prolyl-tRNA synthetase 1
Synonyms: 3010002K18Rik, 2410081F06Rik, Qprs, Eprs
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 107508
HGNC: HGNC:3418
Homologene: 5870
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 162,657,118 bp
  • A to G, chromosome 1 at 185,418,210 bp
  • T to C, chromosome 2 at 32,774,425 bp
  • T to A, chromosome 2 at 76,726,555 bp
  • T to A, chromosome 2 at 76,946,129 bp
  • C to T, chromosome 2 at 119,134,118 bp
  • T to A, chromosome 2 at 125,335,487 bp
  • T to C, chromosome 2 at 151,587,401 bp
  • C to T, chromosome 2 at 154,267,696 bp
  • A to T, chromosome 2 at 155,045,758 bp
  • A to T, chromosome 2 at 172,939,897 bp
  • A to T, chromosome 2 at 178,068,031 bp
  • A to T, chromosome 2 at 180,500,932 bp
  • G to A, chromosome 3 at 67,440,181 bp
  • A to G, chromosome 4 at 6,438,083 bp
  • A to G, chromosome 4 at 45,285,700 bp
  • A to T, chromosome 4 at 49,490,275 bp
  • C to T, chromosome 5 at 34,639,032 bp
  • T to A, chromosome 5 at 87,578,653 bp
  • G to C, chromosome 5 at 88,554,517 bp
  • T to A, chromosome 5 at 90,462,834 bp
  • A to G, chromosome 5 at 122,334,278 bp
  • G to T, chromosome 5 at 123,627,356 bp
  • T to C, chromosome 6 at 122,840,231 bp
  • A to T, chromosome 6 at 127,148,762 bp
  • A to T, chromosome 6 at 138,147,756 bp
  • A to T, chromosome 7 at 19,149,130 bp
  • A to T, chromosome 7 at 28,629,530 bp
  • A to G, chromosome 7 at 46,378,219 bp
  • G to T, chromosome 7 at 81,024,322 bp
  • A to T, chromosome 7 at 114,030,240 bp
  • C to A, chromosome 8 at 71,489,495 bp
  • G to A, chromosome 8 at 106,010,844 bp
  • T to G, chromosome 8 at 122,638,029 bp
  • T to C, chromosome 9 at 32,322,791 bp
  • A to T, chromosome 9 at 38,473,443 bp
  • T to C, chromosome 10 at 21,310,152 bp
  • G to A, chromosome 10 at 53,062,541 bp
  • A to T, chromosome 10 at 118,294,943 bp
  • A to G, chromosome 11 at 100,472,242 bp
  • T to C, chromosome 11 at 102,438,238 bp
  • T to A, chromosome 12 at 34,985,680 bp
  • T to C, chromosome 12 at 78,492,102 bp
  • G to T, chromosome 12 at 88,431,045 bp
  • T to C, chromosome 12 at 110,664,749 bp
  • T to C, chromosome 13 at 23,571,769 bp
  • T to G, chromosome 13 at 63,819,775 bp
  • T to A, chromosome 13 at 89,131,783 bp
  • T to A, chromosome 13 at 91,694,003 bp
  • A to T, chromosome 14 at 16,508,932 bp
  • T to C, chromosome 14 at 50,238,616 bp
  • T to A, chromosome 14 at 54,186,149 bp
  • A to G, chromosome 15 at 81,414,534 bp
  • A to G, chromosome 15 at 102,938,375 bp
  • G to A, chromosome 16 at 87,472,089 bp
  • T to A, chromosome 17 at 12,657,005 bp
  • G to A, chromosome 17 at 35,618,869 bp
  • G to T, chromosome 17 at 56,622,495 bp
  • T to A, chromosome 18 at 36,968,136 bp
  • T to C, chromosome 18 at 37,006,926 bp
  • T to A, chromosome 18 at 58,837,883 bp
  • A to G, chromosome 19 at 17,119,897 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7295 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045363-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.