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Strain Name:
C57BL/6J-MtgxR7304Btlr/Mmmh
Stock Number:
045365-MU
Citation ID:
RRID:MMRRC_045365-MU
Other Names:
R7304 (G1)
Major Collection:

Strain Information

Col3a1
Name: collagen, type III, alpha 1
Synonyms: Col3a-1, Tsk-2, Tsk2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12825
HGNC: HGNC:2201
Homologene: 55433
Vldlr
Name: very low density lipoprotein receptor
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 22359
Homologene: 443
Ppp1r13b
Name: protein phosphatase 1, regulatory subunit 13B
Synonyms: ASPP1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 21981
VEGA: 12
Homologene: 9090
Depdc7
Name: DEP domain containing 7
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 211896
Homologene: 16386
Acap2
Name: ArfGAP with coiled-coil, ankyrin repeat and PH domains 2
Synonyms: 9530039J15Rik, Centb2
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 78618
VEGA: 16
Homologene: 8182
Armc9
Name: armadillo repeat containing 9
Synonyms: 5730415N24Rik, 3830422A13Rik, 4831423D23Rik, 4930438O05Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 78795
Homologene: 11847
Dido1
Name: death inducer-obliterator 1
Synonyms: DIO-1, 6720461J16Rik, D130048F08Rik, Datf1, dido, C130092D22Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 23856
HGNC: HGNC:2680
Homologene: 34139
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 37,164,505 bp
  • A to T, chromosome 1 at 45,347,811 bp
  • C to T, chromosome 1 at 75,479,054 bp
  • C to G, chromosome 1 at 86,162,715 bp
  • A to T, chromosome 1 at 120,022,240 bp
  • A to G, chromosome 1 at 130,103,153 bp
  • A to T, chromosome 1 at 173,642,590 bp
  • A to T, chromosome 2 at 22,853,135 bp
  • A to G, chromosome 2 at 104,723,118 bp
  • G to A, chromosome 2 at 180,687,493 bp
  • T to C, chromosome 3 at 64,504,250 bp
  • G to A, chromosome 3 at 82,009,565 bp
  • A to G, chromosome 3 at 107,104,750 bp
  • A to G, chromosome 4 at 66,185,375 bp
  • C to G, chromosome 4 at 99,769,521 bp
  • T to A, chromosome 4 at 103,139,402 bp
  • T to C, chromosome 4 at 117,957,436 bp
  • T to C, chromosome 4 at 123,342,389 bp
  • G to A, chromosome 4 at 126,125,690 bp
  • T to C, chromosome 5 at 65,619,734 bp
  • T to C, chromosome 6 at 116,640,317 bp
  • T to A, chromosome 6 at 131,685,042 bp
  • T to C, chromosome 7 at 3,737,494 bp
  • T to A, chromosome 7 at 4,994,583 bp
  • T to C, chromosome 7 at 11,756,897 bp
  • T to C, chromosome 7 at 12,810,899 bp
  • T to A, chromosome 7 at 44,629,991 bp
  • T to C, chromosome 7 at 100,645,667 bp
  • T to A, chromosome 7 at 102,106,324 bp
  • T to C, chromosome 7 at 110,899,724 bp
  • T to C, chromosome 7 at 121,937,139 bp
  • T to C, chromosome 8 at 22,639,959 bp
  • T to A, chromosome 8 at 72,100,346 bp
  • T to C, chromosome 8 at 112,011,648 bp
  • A to G, chromosome 9 at 96,687,427 bp
  • A to G, chromosome 9 at 102,728,350 bp
  • G to T, chromosome 9 at 104,238,514 bp
  • A to G, chromosome 9 at 121,416,212 bp
  • A to G, chromosome 10 at 20,099,555 bp
  • C to T, chromosome 10 at 43,997,258 bp
  • A to G, chromosome 11 at 9,297,203 bp
  • T to C, chromosome 11 at 97,551,693 bp
  • T to G, chromosome 11 at 100,257,337 bp
  • T to A, chromosome 11 at 119,337,747 bp
  • C to A, chromosome 12 at 54,069,041 bp
  • A to G, chromosome 12 at 55,304,644 bp
  • T to C, chromosome 12 at 57,576,051 bp
  • C to T, chromosome 12 at 103,063,190 bp
  • T to C, chromosome 12 at 111,872,406 bp
  • T to A, chromosome 12 at 117,248,544 bp
  • A to T, chromosome 14 at 4,000,520 bp
  • T to A, chromosome 14 at 29,022,118 bp
  • T to A, chromosome 14 at 53,351,402 bp
  • T to A, chromosome 14 at 70,776,028 bp
  • G to A, chromosome 15 at 36,254,452 bp
  • A to T, chromosome 15 at 44,498,482 bp
  • A to G, chromosome 15 at 85,871,547 bp
  • A to C, chromosome 16 at 31,108,116 bp
  • G to A, chromosome 16 at 33,760,790 bp
  • A to T, chromosome 16 at 94,941,183 bp
  • G to T, chromosome 17 at 20,687,749 bp
  • T to C, chromosome 17 at 25,851,506 bp
  • A to T, chromosome 17 at 45,560,141 bp
  • T to C, chromosome 17 at 47,672,871 bp
  • A to T, chromosome 18 at 36,045,941 bp
  • C to T, chromosome 19 at 5,716,382 bp
  • T to A, chromosome 19 at 27,238,604 bp
  • T to C, chromosome 19 at 28,964,217 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7304 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045365-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.