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Strain Name:
C57BL/6J-MtgxR7349Btlr/Mmmh
Stock Number:
045435-MU
Citation ID:
RRID:MMRRC_045435-MU
Other Names:
R7349 (G1)
Major Collection:

Strain Information

Angpt2
Name: angiopoietin 2
Synonyms: Ang2, Ang-2
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 11601
HGNC: HGNC:485
Homologene: 22401
Htr3b
Name: 5-hydroxytryptamine (serotonin) receptor 3B
Synonyms: 5-HT3 receptor subunit B, 5-HT3B
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 57014
VEGA: 9
HGNC: HGNC:5298
Homologene: 38131
Herc3
Name: hect domain and RLD 3
Synonyms: 5730409F18Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 73998
HGNC: HGNC:4876
Homologene: 57095
Ptprk
Name: protein tyrosine phosphatase receptor type K
Synonyms: PTPk, RPTPkappa
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 19272
VEGA: 10
HGNC: HGNC:9674
Homologene: 55693
Rnf220
Name: ring finger protein 220
Synonyms: 5730503K05Rik, 4931406I20Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 66743
Homologene: 10036
Map1b
Name: microtubule-associated protein 1B
Synonyms: MAP5, Mtap-5, Mtap5, LC1, Mtap1b
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 17755
VEGA: 13
HGNC: HGNC:6836
Homologene: 38111
Pdzd2
Name: PDZ domain containing 2
Synonyms: 4930537L06Rik, LOC223364, A930022H17Rik, Pdzk3, Gm21706
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 68070
Homologene: 23393
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to G, chromosome 1 at 44,180,908 bp
  • T to C, chromosome 1 at 92,597,182 bp
  • T to G, chromosome 1 at 133,897,879 bp
  • T to C, chromosome 2 at 125,315,401 bp
  • A to G, chromosome 2 at 126,325,345 bp
  • A to T, chromosome 3 at 15,410,604 bp
  • A to T, chromosome 3 at 15,832,146 bp
  • A to T, chromosome 3 at 59,258,325 bp
  • T to A, chromosome 3 at 93,220,206 bp
  • T to C, chromosome 3 at 152,747,601 bp
  • T to G, chromosome 4 at 48,051,290 bp
  • G to A, chromosome 4 at 117,277,818 bp
  • T to C, chromosome 5 at 5,555,379 bp
  • T to C, chromosome 5 at 121,310,663 bp
  • T to A, chromosome 5 at 124,712,328 bp
  • C to T, chromosome 5 at 135,889,333 bp
  • T to A, chromosome 5 at 143,925,836 bp
  • A to T, chromosome 5 at 151,562,217 bp
  • C to A, chromosome 6 at 12,352,068 bp
  • T to C, chromosome 6 at 58,858,986 bp
  • G to A, chromosome 7 at 4,921,818 bp
  • T to C, chromosome 7 at 101,290,266 bp
  • T to C, chromosome 7 at 101,390,228 bp
  • A to G, chromosome 7 at 104,564,650 bp
  • A to G, chromosome 7 at 105,710,123 bp
  • T to C, chromosome 7 at 127,482,799 bp
  • A to T, chromosome 7 at 131,041,124 bp
  • T to A, chromosome 8 at 18,692,074 bp
  • A to T, chromosome 8 at 45,795,823 bp
  • T to C, chromosome 8 at 85,079,831 bp
  • G to A, chromosome 8 at 105,452,268 bp
  • T to C, chromosome 8 at 109,928,646 bp
  • A to G, chromosome 8 at 110,398,171 bp
  • A to G, chromosome 8 at 119,242,358 bp
  • T to C, chromosome 9 at 5,345,527 bp
  • C to T, chromosome 9 at 21,921,114 bp
  • C to T, chromosome 9 at 48,936,019 bp
  • T to G, chromosome 9 at 49,447,967 bp
  • T to C, chromosome 9 at 57,044,376 bp
  • T to A, chromosome 9 at 104,291,458 bp
  • T to C, chromosome 9 at 108,110,783 bp
  • G to T, chromosome 10 at 3,545,638 bp
  • A to T, chromosome 10 at 28,592,838 bp
  • T to C, chromosome 10 at 76,285,592 bp
  • T to C, chromosome 10 at 127,492,646 bp
  • G to A, chromosome 11 at 59,548,086 bp
  • T to G, chromosome 11 at 71,182,117 bp
  • A to T, chromosome 11 at 90,592,111 bp
  • A to T, chromosome 11 at 100,521,991 bp
  • A to G, chromosome 11 at 103,387,334 bp
  • G to A, chromosome 11 at 106,249,746 bp
  • A to G, chromosome 12 at 76,564,565 bp
  • C to A, chromosome 13 at 3,556,395 bp
  • T to C, chromosome 13 at 54,784,193 bp
  • T to A, chromosome 13 at 60,842,903 bp
  • C to T, chromosome 13 at 93,752,233 bp
  • T to A, chromosome 13 at 99,433,640 bp
  • C to A, chromosome 14 at 43,230,601 bp
  • G to A, chromosome 14 at 103,543,879 bp
  • C to G, chromosome 15 at 12,399,205 bp
  • A to G, chromosome 15 at 44,514,954 bp
  • A to T, chromosome 15 at 72,736,869 bp
  • T to A, chromosome 16 at 5,104,729 bp
  • A to G, chromosome 16 at 36,364,312 bp
  • T to A, chromosome 17 at 27,107,812 bp
  • T to C, chromosome 17 at 74,373,324 bp
  • G to A, chromosome 17 at 86,493,355 bp
  • A to G, chromosome 18 at 46,169,428 bp
  • T to C, chromosome 19 at 4,841,840 bp
  • T to A, chromosome 19 at 11,478,191 bp
  • T to C, chromosome 19 at 45,010,161 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7349 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045435-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.