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Strain Detail Sheet

Strain Name:
C57BL/6J-MtgxR7354Btlr/Mmmh
Stock Number:
045440-MU
Citation ID:
RRID:MMRRC_045440-MU
Other Names:
R7354 (G1)
Major Collection:
Beutler Mutagenetix

Strain Information

Gene Details

Mtrr
Name: 5-methyltetrahydrofolate-homocysteine methyltransferase reductase
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 210009
VEGA: 13
HGNC: HGNC:7473
Homologene: 11419

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Itga6
Name: integrin alpha 6
Synonyms: Cd49f, 5033401O05Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 16403
HGNC: HGNC:6142
Homologene: 20091

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IMPC
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Gene Ontology
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Pcdh9
Name: protocadherin 9
Synonyms: C630029H24Rik, 1500001L12Rik, LOC382930, A730003J17Rik, C530050I23Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 211712
HGNC: HGNC:8661
Homologene: 10698

Additional Resources
IMPC
Google
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IMSR
Gene Ontology
BioGPS
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Mouse Mine
KOMP Phenotyping
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ClinGen
Arid4b
Name: AT-rich interaction domain 4B
Synonyms: RBBP1L1, SAP180, BRCAA1, Rbp1l1, 6720480E17Rik, 6330417L24Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 94246
Homologene: 12847

Additional Resources
IMPC
Google
Entrez
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IMSR
Gene Ontology
BioGPS
Gene Cloud
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Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
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ClinGen
Top1
Name: topoisomerase (DNA) I
Synonyms: Top-1, D130064I21Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 21969
Homologene: 2467

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Fancd2
Name: Fanconi anemia, complementation group D2
Synonyms: 2410150O07Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 211651
HGNC: HGNC:3585
Homologene: 13212

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Fam117a
Name: family with sequence similarity 117, member A
Synonyms: 5730593F17Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 215512
Homologene: 12775

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 58,469,747 bp
  • A to G, chromosome 1 at 107,377,533 bp
  • A to G, chromosome 1 at 135,976,032 bp
  • A to T, chromosome 1 at 150,806,445 bp
  • G to T, chromosome 1 at 156,036,113 bp
  • G to A, chromosome 2 at 71,820,230 bp
  • A to T, chromosome 2 at 80,446,809 bp
  • T to C, chromosome 2 at 89,248,687 bp
  • C to T, chromosome 2 at 111,537,564 bp
  • C to A, chromosome 2 at 120,102,132 bp
  • T to C, chromosome 2 at 160,704,958 bp
  • T to C, chromosome 2 at 167,474,131 bp
  • T to C, chromosome 2 at 182,000,686 bp
  • T to A, chromosome 3 at 97,719,330 bp
  • G to A, chromosome 3 at 101,864,581 bp
  • T to C, chromosome 3 at 152,290,500 bp
  • T to A, chromosome 4 at 126,417,306 bp
  • G to T, chromosome 4 at 133,693,947 bp
  • T to C, chromosome 4 at 152,314,091 bp
  • G to T, chromosome 4 at 155,465,347 bp
  • A to T, chromosome 5 at 33,250,388 bp
  • G to A, chromosome 5 at 38,682,779 bp
  • G to A, chromosome 5 at 137,768,109 bp
  • A to T, chromosome 5 at 146,583,962 bp
  • A to T, chromosome 6 at 40,744,798 bp
  • T to A, chromosome 6 at 83,060,976 bp
  • T to A, chromosome 6 at 98,238,281 bp
  • A to G, chromosome 6 at 113,595,946 bp
  • C to A, chromosome 6 at 115,935,503 bp
  • T to G, chromosome 7 at 19,393,654 bp
  • T to A, chromosome 7 at 31,060,622 bp
  • C to T, chromosome 7 at 116,083,911 bp
  • A to T, chromosome 7 at 126,448,856 bp
  • G to A, chromosome 7 at 131,256,729 bp
  • G to T, chromosome 7 at 131,272,033 bp
  • A to C, chromosome 7 at 140,504,186 bp
  • T to A, chromosome 8 at 19,250,734 bp
  • C to A, chromosome 8 at 70,889,603 bp
  • A to G, chromosome 8 at 84,228,849 bp
  • T to C, chromosome 8 at 85,062,196 bp
  • A to G, chromosome 9 at 110,629,179 bp
  • T to C, chromosome 10 at 40,076,955 bp
  • A to G, chromosome 10 at 77,782,533 bp
  • A to C, chromosome 10 at 79,356,630 bp
  • T to C, chromosome 10 at 127,571,408 bp
  • T to A, chromosome 10 at 128,254,738 bp
  • A to G, chromosome 11 at 65,187,493 bp
  • C to A, chromosome 11 at 67,096,882 bp
  • A to T, chromosome 11 at 71,181,550 bp
  • A to T, chromosome 11 at 73,375,375 bp
  • A to G, chromosome 11 at 95,380,703 bp
  • G to A, chromosome 11 at 115,928,201 bp
  • T to C, chromosome 12 at 3,455,637 bp
  • A to G, chromosome 12 at 4,096,249 bp
  • A to T, chromosome 12 at 102,838,205 bp
  • C to T, chromosome 12 at 103,142,702 bp
  • A to G, chromosome 12 at 109,033,289 bp
  • A to G, chromosome 13 at 14,164,870 bp
  • A to T, chromosome 13 at 38,047,312 bp
  • T to C, chromosome 13 at 49,704,320 bp
  • A to T, chromosome 13 at 59,741,834 bp
  • A to T, chromosome 13 at 68,566,207 bp
  • T to C, chromosome 13 at 96,394,525 bp
  • T to G, chromosome 13 at 100,677,874 bp
  • T to C, chromosome 14 at 93,888,270 bp
  • A to G, chromosome 14 at 99,047,358 bp
  • A to G, chromosome 15 at 89,165,725 bp
  • T to C, chromosome 16 at 3,987,099 bp
  • C to T, chromosome 16 at 11,148,514 bp
  • T to C, chromosome 16 at 30,065,928 bp
  • A to T, chromosome 17 at 20,540,781 bp
  • A to G, chromosome 17 at 37,924,393 bp
  • G to A, chromosome 17 at 40,319,180 bp
  • A to G, chromosome 17 at 56,104,427 bp
  • C to T, chromosome 17 at 64,752,544 bp
  • A to G, chromosome 18 at 34,905,987 bp
  • G to A, chromosome 18 at 37,478,124 bp
  • A to G, chromosome 18 at 37,520,258 bp
  • A to T, chromosome 18 at 70,241,300 bp
  • A to G, chromosome 18 at 73,873,314 bp

Strain Description

Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.

Strain Origin

Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7354 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
Black

Breeding

MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain

Reproductive Statistics

Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
045440-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.