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Strain Name:
C57BL/6J-MtgxR7359Btlr/Mmmh
Stock Number:
045445-MU
Citation ID:
RRID:MMRRC_045445-MU
Other Names:
R7359 (G1)
Major Collection:

Strain Information

Vps33a
Name: VPS33A CORVET/HOPS core subunit
Synonyms: bf, 3830421M04Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 77573
Homologene: 11294
En1
Name: engrailed 1
Synonyms: Mo-en.1, En-1, engrailed-1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13798
HGNC: HGNC:3342
Homologene: 50663
Zfp423
Name: zinc finger protein 423
Synonyms: Roaz, ataxia1, Zfp104, Ebfaz
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 94187
Homologene: 9010
Pcif1
Name: phosphorylated CTD interacting factor 1
Synonyms: 2310022K11Rik, F730014I05Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228866
Homologene: 11134
Dnajc24
Name: DnaJ heat shock protein family (Hsp40) member C24
Synonyms: MmDjC7, 2610027M02Rik, 1700030A21Rik, Zcsl3, Dph4
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 99349
Homologene: 41704
Dock5
Name: dedicator of cytokinesis 5
Synonyms: lr2, 1110060D06Rik, rlc
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 68813
VEGA: 14
Homologene: 57016
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 43,075,533 bp
  • C to T, chromosome 1 at 75,360,952 bp
  • T to A, chromosome 1 at 80,709,348 bp
  • A to G, chromosome 1 at 120,607,088 bp
  • A to G, chromosome 1 at 178,187,943 bp
  • T to A, chromosome 2 at 24,048,719 bp
  • A to T, chromosome 2 at 31,388,383 bp
  • A to T, chromosome 2 at 37,005,437 bp
  • C to A, chromosome 2 at 74,764,103 bp
  • T to A, chromosome 2 at 87,358,211 bp
  • C to A, chromosome 2 at 104,214,137 bp
  • T to C, chromosome 2 at 106,001,948 bp
  • A to G, chromosome 2 at 120,698,280 bp
  • T to C, chromosome 2 at 121,340,848 bp
  • T to C, chromosome 2 at 137,084,306 bp
  • A to G, chromosome 2 at 151,494,359 bp
  • A to G, chromosome 2 at 164,884,331 bp
  • A to T, chromosome 3 at 15,833,225 bp
  • A to G, chromosome 3 at 95,240,623 bp
  • A to G, chromosome 4 at 11,273,125 bp
  • T to A, chromosome 4 at 34,565,944 bp
  • G to A, chromosome 4 at 81,356,395 bp
  • T to C, chromosome 4 at 95,769,480 bp
  • T to A, chromosome 4 at 118,598,965 bp
  • A to T, chromosome 5 at 88,665,564 bp
  • A to G, chromosome 5 at 115,926,574 bp
  • A to G, chromosome 5 at 123,558,633 bp
  • T to C, chromosome 6 at 18,221,624 bp
  • A to C, chromosome 6 at 43,280,282 bp
  • C to T, chromosome 6 at 48,765,346 bp
  • T to C, chromosome 6 at 57,138,199 bp
  • A to G, chromosome 6 at 57,405,095 bp
  • G to A, chromosome 6 at 84,195,324 bp
  • T to A, chromosome 6 at 125,566,257 bp
  • G to T, chromosome 6 at 134,450,960 bp
  • T to C, chromosome 6 at 139,967,894 bp
  • G to A, chromosome 6 at 142,671,682 bp
  • T to A, chromosome 7 at 3,898,104 bp
  • T to C, chromosome 7 at 4,427,697 bp
  • A to T, chromosome 7 at 8,367,539 bp
  • T to C, chromosome 7 at 24,781,007 bp
  • T to C, chromosome 7 at 27,808,235 bp
  • T to G, chromosome 7 at 28,841,299 bp
  • A to C, chromosome 7 at 42,265,983 bp
  • A to G, chromosome 7 at 141,071,284 bp
  • T to C, chromosome 8 at 15,028,858 bp
  • T to C, chromosome 8 at 87,782,243 bp
  • CT to C, chromosome 8 at 104,182,032 bp
  • C to G, chromosome 8 at 110,506,101 bp
  • A to T, chromosome 9 at 18,643,020 bp
  • T to C, chromosome 9 at 39,365,479 bp
  • A to T, chromosome 9 at 110,562,944 bp
  • T to C, chromosome 9 at 110,986,296 bp
  • T to C, chromosome 10 at 53,699,222 bp
  • A to T, chromosome 10 at 74,584,216 bp
  • A to G, chromosome 10 at 99,264,065 bp
  • A to C, chromosome 10 at 122,449,378 bp
  • T to A, chromosome 11 at 69,998,641 bp
  • T to C, chromosome 11 at 78,058,557 bp
  • A to G, chromosome 11 at 82,028,231 bp
  • A to G, chromosome 11 at 106,018,963 bp
  • T to C, chromosome 12 at 71,189,574 bp
  • T to A, chromosome 12 at 81,150,701 bp
  • T to C, chromosome 12 at 110,624,602 bp
  • T to G, chromosome 13 at 24,468,556 bp
  • T to C, chromosome 13 at 47,105,589 bp
  • A to G, chromosome 13 at 54,503,918 bp
  • A to T, chromosome 13 at 55,321,275 bp
  • A to G, chromosome 13 at 59,712,490 bp
  • A to G, chromosome 13 at 95,604,686 bp
  • T to C, chromosome 13 at 96,082,462 bp
  • T to C, chromosome 13 at 113,342,430 bp
  • A to G, chromosome 14 at 16,407,376 bp
  • T to C, chromosome 14 at 24,164,547 bp
  • A to G, chromosome 14 at 67,765,888 bp
  • G to T, chromosome 15 at 44,589,486 bp
  • T to A, chromosome 15 at 57,916,108 bp
  • C to T, chromosome 15 at 99,631,200 bp
  • G to A, chromosome 15 at 103,521,325 bp
  • A to G, chromosome 16 at 5,089,341 bp
  • A to T, chromosome 17 at 7,813,486 bp
  • T to C, chromosome 17 at 28,881,185 bp
  • G to A, chromosome 17 at 38,067,615 bp
  • T to C, chromosome 17 at 42,667,112 bp
  • A to G, chromosome 17 at 47,699,525 bp
  • T to C, chromosome 18 at 6,065,709 bp
  • T to C, chromosome 18 at 12,195,180 bp
  • T to C, chromosome 18 at 34,641,341 bp
  • C to T, chromosome 18 at 69,956,052 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7359 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045445-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.