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Strain Name:
C57BL/6J-MtgxR7369Btlr/Mmmh
Stock Number:
045453-MU
Citation ID:
RRID:MMRRC_045453-MU
Other Names:
R7369 (G1)
Major Collection:

Strain Information

Evl
Name: Ena-vasodilator stimulated phosphoprotein
Synonyms: b2b2600Clo
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 14026
VEGA: 12
Homologene: 56752
Sort1
Name: sortilin 1
Synonyms: sortilin, neurotensin receptor 3, Ntsr3, Ntr3, 2900053A11Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 20661
Homologene: 136097
Pcdh9
Name: protocadherin 9
Synonyms: C630029H24Rik, 1500001L12Rik, LOC382930, A730003J17Rik, C530050I23Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 211712
HGNC: HGNC:8661
Homologene: 10698
Cdyl
Name: chromodomain protein, Y chromosome-like
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 12593
VEGA: 13
HGNC: HGNC:1811
Homologene: 3548
Uba2
Name: ubiquitin-like modifier activating enzyme 2
Synonyms: Sumo-1 activating enzyme subunit 2, UBA2, anthracycline-associated resistance, Arx, SAE2, Uble1b
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 50995
Homologene: 4018
Polr2a
Name: polymerase (RNA) II (DNA directed) polypeptide A
Synonyms: 220kDa, Rpo2-1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20020
HGNC: HGNC:9187
Homologene: 721
Erp44
Name: endoplasmic reticulum protein 44
Synonyms: 1110001E24Rik, Txndc4
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 76299
Homologene: 12638
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 44,180,860 bp
  • T to C, chromosome 1 at 64,121,141 bp
  • G to A, chromosome 1 at 87,921,622 bp
  • A to G, chromosome 1 at 106,875,149 bp
  • A to T, chromosome 1 at 117,986,108 bp
  • A to T, chromosome 1 at 130,841,766 bp
  • C to T, chromosome 1 at 155,197,384 bp
  • T to G, chromosome 1 at 170,922,317 bp
  • T to C, chromosome 2 at 26,667,894 bp
  • T to A, chromosome 2 at 32,069,687 bp
  • C to T, chromosome 2 at 41,282,039 bp
  • T to C, chromosome 2 at 51,950,084 bp
  • A to G, chromosome 2 at 181,198,461 bp
  • C to T, chromosome 3 at 87,141,084 bp
  • T to C, chromosome 3 at 87,256,701 bp
  • G to A, chromosome 3 at 92,686,083 bp
  • G to A, chromosome 3 at 108,351,680 bp
  • T to C, chromosome 4 at 12,053,535 bp
  • T to A, chromosome 4 at 32,773,375 bp
  • T to A, chromosome 4 at 46,013,239 bp
  • T to C, chromosome 4 at 48,218,183 bp
  • A to C, chromosome 5 at 76,593,253 bp
  • A to G, chromosome 5 at 108,445,528 bp
  • T to A, chromosome 6 at 57,159,698 bp
  • T to A, chromosome 6 at 120,432,004 bp
  • T to C, chromosome 6 at 123,815,679 bp
  • T to A, chromosome 6 at 129,331,533 bp
  • T to A, chromosome 6 at 145,413,575 bp
  • T to A, chromosome 7 at 3,653,331 bp
  • C to T, chromosome 7 at 23,660,605 bp
  • T to A, chromosome 7 at 30,105,325 bp
  • C to T, chromosome 7 at 30,289,952 bp
  • A to G, chromosome 7 at 34,150,814 bp
  • A to G, chromosome 7 at 43,351,817 bp
  • A to T, chromosome 8 at 22,561,400 bp
  • T to A, chromosome 8 at 67,904,166 bp
  • A to G, chromosome 8 at 105,300,334 bp
  • T to C, chromosome 8 at 121,923,834 bp
  • C to A, chromosome 8 at 125,911,116 bp
  • T to A, chromosome 8 at 128,431,915 bp
  • T to C, chromosome 9 at 104,018,250 bp
  • T to A, chromosome 9 at 123,056,684 bp
  • A to G, chromosome 10 at 68,041,429 bp
  • A to G, chromosome 10 at 91,001,036 bp
  • A to T, chromosome 10 at 129,463,521 bp
  • T to C, chromosome 11 at 23,432,361 bp
  • T to A, chromosome 11 at 46,616,117 bp
  • A to T, chromosome 11 at 53,465,248 bp
  • A to T, chromosome 11 at 67,220,698 bp
  • A to C, chromosome 11 at 69,745,977 bp
  • T to C, chromosome 11 at 73,077,921 bp
  • G to A, chromosome 11 at 77,117,835 bp
  • T to A, chromosome 11 at 102,399,895 bp
  • A to G, chromosome 11 at 119,430,468 bp
  • A to T, chromosome 12 at 35,504,660 bp
  • A to T, chromosome 12 at 57,672,931 bp
  • T to G, chromosome 12 at 84,292,306 bp
  • A to G, chromosome 12 at 108,686,565 bp
  • T to C, chromosome 12 at 109,033,927 bp
  • A to G, chromosome 13 at 35,816,009 bp
  • G to T, chromosome 13 at 38,197,525 bp
  • A to G, chromosome 13 at 59,782,053 bp
  • T to A, chromosome 13 at 92,299,262 bp
  • C to A, chromosome 14 at 93,886,367 bp
  • T to C, chromosome 15 at 9,584,207 bp
  • A to G, chromosome 15 at 13,042,638 bp
  • A to G, chromosome 15 at 58,053,300 bp
  • T to C, chromosome 15 at 89,454,251 bp
  • G to T, chromosome 15 at 102,553,809 bp
  • C to A, chromosome 16 at 19,769,660 bp
  • A to G, chromosome 16 at 37,134,341 bp
  • A to G, chromosome 16 at 55,821,846 bp
  • A to G, chromosome 17 at 21,723,233 bp
  • G to A, chromosome 17 at 25,947,960 bp
  • T to C, chromosome 18 at 31,886,666 bp
  • A to C, chromosome X at 164,014,840 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7369 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045453-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.