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Strain Name:
C57BL/6J-MtgxR7382Btlr/Mmmh
Stock Number:
045464-MU
Citation ID:
RRID:MMRRC_045464-MU
Other Names:
R7382 (G1)
Major Collection:

Strain Information

Ap4e1
Name: adaptor-related protein complex AP-4, epsilon 1
Synonyms: 2310033A20Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 108011
HGNC: HGNC:573
Homologene: 22397
Smarca4
Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
Synonyms: SNF2beta, Brg1, SW1/SNF, b2b692Clo, b2b508.1Clo
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 20586
Homologene: 135927
Cdon
Name: cell adhesion molecule-related/down-regulated by oncogenes
Synonyms: CAM-related/down-regulated by oncogenes, CDO
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 57810
Homologene: 22996
Syt3
Name: synaptotagmin III
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 20981
Homologene: 9617
Ezh2
Name: enhancer of zeste 2 polycomb repressive complex 2 subunit
Synonyms: Enx-1, Enx1h, KMT6
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 14056
HGNC: HGNC:3527
Homologene: 37926
Ranbp9
Name: RAN binding protein 9
Synonyms: IBAP-1, RanBPM
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 56705
VEGA: 13
Homologene: 38057
Adgrl2
Name: adhesion G protein-coupled receptor L2
Synonyms: Lphh1, Lec1, Lphn2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 99633
Homologene: 22712
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 131,846,632 bp
  • A to G, chromosome 1 at 161,078,461 bp
  • G to A, chromosome 1 at 166,201,598 bp
  • A to T, chromosome 1 at 166,663,660 bp
  • A to T, chromosome 2 at 24,087,314 bp
  • T to C, chromosome 2 at 32,798,168 bp
  • T to C, chromosome 2 at 34,807,302 bp
  • G to T, chromosome 2 at 36,688,034 bp
  • T to C, chromosome 2 at 86,732,785 bp
  • A to G, chromosome 2 at 121,290,785 bp
  • T to A, chromosome 2 at 127,008,902 bp
  • A to T, chromosome 2 at 156,020,749 bp
  • T to C, chromosome 2 at 174,423,383 bp
  • T to A, chromosome 3 at 59,743,616 bp
  • T to G, chromosome 3 at 148,817,283 bp
  • T to A, chromosome 4 at 45,278,880 bp
  • T to C, chromosome 4 at 63,418,336 bp
  • A to T, chromosome 4 at 64,014,043 bp
  • A to T, chromosome 4 at 70,290,025 bp
  • A to T, chromosome 4 at 100,532,182 bp
  • A to T, chromosome 4 at 122,952,123 bp
  • T to A, chromosome 4 at 124,751,577 bp
  • C to A, chromosome 5 at 31,075,829 bp
  • A to T, chromosome 5 at 86,231,864 bp
  • C to A, chromosome 5 at 108,139,007 bp
  • T to C, chromosome 6 at 47,551,836 bp
  • G to T, chromosome 6 at 55,978,419 bp
  • C to A, chromosome 6 at 70,044,119 bp
  • C to T, chromosome 6 at 119,239,087 bp
  • A to G, chromosome 6 at 124,197,317 bp
  • A to G, chromosome 6 at 124,311,312 bp
  • A to G, chromosome 7 at 29,204,844 bp
  • A to T, chromosome 7 at 44,392,746 bp
  • T to A, chromosome 7 at 139,990,107 bp
  • T to C, chromosome 7 at 141,858,948 bp
  • A to T, chromosome 8 at 3,877,965 bp
  • T to C, chromosome 8 at 70,242,844 bp
  • G to A, chromosome 8 at 85,050,119 bp
  • G to T, chromosome 8 at 117,054,871 bp
  • A to G, chromosome 9 at 21,658,933 bp
  • A to G, chromosome 9 at 35,478,648 bp
  • G to T, chromosome 9 at 40,211,505 bp
  • A to C, chromosome 9 at 55,163,014 bp
  • A to C, chromosome 9 at 75,304,050 bp
  • A to T, chromosome 9 at 97,799,398 bp
  • G to A, chromosome 9 at 119,074,691 bp
  • T to A, chromosome 10 at 19,356,665 bp
  • G to T, chromosome 10 at 62,997,969 bp
  • A to T, chromosome 10 at 79,067,269 bp
  • T to C, chromosome 10 at 81,720,825 bp
  • A to G, chromosome 10 at 86,776,510 bp
  • A to G, chromosome 11 at 31,872,828 bp
  • T to C, chromosome 11 at 69,641,556 bp
  • A to T, chromosome 11 at 78,404,795 bp
  • A to T, chromosome 12 at 35,504,515 bp
  • A to G, chromosome 12 at 53,142,171 bp
  • T to G, chromosome 13 at 22,772,586 bp
  • C to T, chromosome 13 at 43,425,114 bp
  • G to A, chromosome 14 at 59,654,594 bp
  • T to G, chromosome 14 at 118,162,623 bp
  • C to T, chromosome 15 at 85,787,228 bp
  • T to A, chromosome 15 at 97,718,926 bp
  • C to T, chromosome 16 at 4,988,482 bp
  • TTGTG to TTG, chromosome 16 at 14,300,145 bp
  • T to C, chromosome 16 at 55,246,683 bp
  • G to A, chromosome 16 at 96,205,893 bp
  • T to C, chromosome 17 at 23,743,552 bp
  • G to T, chromosome 17 at 24,705,291 bp
  • A to G, chromosome 17 at 70,787,174 bp
  • G to T, chromosome 17 at 78,803,507 bp
  • A to T, chromosome 18 at 12,201,706 bp
  • A to T, chromosome 18 at 35,325,413 bp
  • A to C, chromosome 18 at 63,017,519 bp
  • T to C, chromosome 19 at 16,619,485 bp
  • T to C, chromosome 19 at 30,071,388 bp
  • T to C, chromosome 19 at 32,159,782 bp
  • G to T, chromosome 19 at 42,044,972 bp
  • T to A, chromosome 19 at 55,926,740 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7382 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045464-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.