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Strain Detail Sheet

Strain Name:
C57BL/6J-MtgxR7386Btlr/Mmmh
Stock Number:
045468-MU
Citation ID:
RRID:MMRRC_045468-MU
Other Names:
R7386 (G1)
Major Collection:
Beutler Mutagenetix

Strain Information

Gene Details

Iqgap1
Name: IQ motif containing GTPase activating protein 1
Synonyms: D7Ertd237e, D7Ertd257e
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 29875
HGNC: HGNC:6110
Homologene: 74514

Additional Resources
IMPC
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Gene Ontology
BioGPS
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Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
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ClinGen
Ncaph2
Name: non-SMC condensin II complex, subunit H2
Synonyms: 0610010J20Rik, 2610524G04Rik, D15Ertd785e, Kleisin beta
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 52683
Homologene: 12045

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
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ClinGen
Foxo3
Name: forkhead box O3
Synonyms: FKHRL1, Fkhr2, 2010203A17Rik, 1110048B16Rik, Foxo3a
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 56484
HGNC: HGNC:3821
Homologene: 31039

Additional Resources
IMPC
Google
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PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
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ClinGen
Depdc5
Name: DEP domain containing 5
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 277854
Homologene: 34718

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
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Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
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ClinGen
Btaf1
Name: B-TFIID TATA-box binding protein associated factor 1
Synonyms: E430027O22Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 107182
Homologene: 31978

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Rtn4
Name: reticulon 4
Synonyms: 1110020G17Rik, C130026I10Rik, NOGO, NgA, Nogo-B, Nogo-A
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 68585
Homologene: 10743

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Sulf1
Name: sulfatase 1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 240725
Homologene: 49408

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 12,838,361 bp
  • T to C, chromosome 1 at 151,471,380 bp
  • A to G, chromosome 1 at 162,548,154 bp
  • G to T, chromosome 1 at 171,503,533 bp
  • T to G, chromosome 2 at 29,786,595 bp
  • T to A, chromosome 2 at 66,540,550 bp
  • A to G, chromosome 2 at 86,159,530 bp
  • T to G, chromosome 3 at 89,432,821 bp
  • T to A, chromosome 4 at 63,095,385 bp
  • G to A, chromosome 4 at 116,641,054 bp
  • A to G, chromosome 5 at 32,927,936 bp
  • C to A, chromosome 5 at 107,809,823 bp
  • A to G, chromosome 5 at 127,563,926 bp
  • G to A, chromosome 6 at 47,858,950 bp
  • T to A, chromosome 7 at 6,305,950 bp
  • A to G, chromosome 7 at 18,858,290 bp
  • A to T, chromosome 7 at 45,314,640 bp
  • A to G, chromosome 7 at 46,714,923 bp
  • A to T, chromosome 7 at 66,968,849 bp
  • A to G, chromosome 7 at 80,726,042 bp
  • A to C, chromosome 7 at 81,974,492 bp
  • A to C, chromosome 7 at 104,768,307 bp
  • G to A, chromosome 7 at 131,112,236 bp
  • A to T, chromosome 7 at 139,976,052 bp
  • A to G, chromosome 8 at 3,487,079 bp
  • G to T, chromosome 8 at 25,754,290 bp
  • A to G, chromosome 8 at 61,532,052 bp
  • A to G, chromosome 8 at 83,610,303 bp
  • T to C, chromosome 9 at 67,028,167 bp
  • T to A, chromosome 9 at 104,872,740 bp
  • A to G, chromosome 9 at 110,373,169 bp
  • A to T, chromosome 10 at 10,377,949 bp
  • A to G, chromosome 10 at 28,789,747 bp
  • T to C, chromosome 10 at 42,197,360 bp
  • T to A, chromosome 10 at 69,822,249 bp
  • T to A, chromosome 10 at 78,916,843 bp
  • T to A, chromosome 11 at 29,707,772 bp
  • T to C, chromosome 11 at 49,444,400 bp
  • C to G, chromosome 11 at 69,003,774 bp
  • A to G, chromosome 11 at 108,808,681 bp
  • T to A, chromosome 11 at 117,721,954 bp
  • A to T, chromosome 11 at 120,408,881 bp
  • T to C, chromosome 13 at 30,906,663 bp
  • A to T, chromosome 13 at 61,000,559 bp
  • A to T, chromosome 13 at 67,670,250 bp
  • A to T, chromosome 13 at 73,689,891 bp
  • C to A, chromosome 13 at 84,222,418 bp
  • T to A, chromosome 13 at 93,069,323 bp
  • T to G, chromosome 14 at 55,497,747 bp
  • T to C, chromosome 14 at 55,778,327 bp
  • T to A, chromosome 15 at 30,966,768 bp
  • G to T, chromosome 15 at 38,296,949 bp
  • G to A, chromosome 15 at 59,330,754 bp
  • G to A, chromosome 15 at 89,370,256 bp
  • C to A, chromosome 16 at 44,789,848 bp
  • T to C, chromosome 16 at 76,293,887 bp
  • A to G, chromosome 17 at 14,673,150 bp
  • T to C, chromosome 17 at 23,177,007 bp
  • A to C, chromosome 17 at 80,267,577 bp
  • C to T, chromosome 18 at 20,041,926 bp
  • T to C, chromosome 18 at 61,821,994 bp
  • A to T, chromosome 18 at 68,950,137 bp
  • G to A, chromosome 19 at 4,663,558 bp
  • A to G, chromosome 19 at 21,409,886 bp
  • G to A, chromosome 19 at 23,215,175 bp
  • G to A, chromosome 19 at 36,958,382 bp

Strain Description

Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.

Strain Origin

Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7386 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
Black

Breeding

MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain

Reproductive Statistics

Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
045468-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.