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Strain Detail Sheet

Strain Name:
C57BL/6J-MtgxR7408Btlr/Mmmh
Stock Number:
045489-MU
Citation ID:
RRID:MMRRC_045489-MU
Other Names:
R7408 (G1)
Major Collection:
Beutler Mutagenetix

Strain Information

Gene Details

Nos1
Name: nitric oxide synthase 1, neuronal
Synonyms: nNOS, bNOS, Nos-1, NO, 2310005C01Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 18125
HGNC: HGNC:7872
Homologene: 37327

Additional Resources
IMPC
Google
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IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Sez6
Name: seizure related gene 6
Synonyms: sez-6, D11Bhm177e
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20370
Homologene: 10948

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Pkp4
Name: plakophilin 4
Synonyms: p0071, Armrp, 5031422I09Rik, 9430019K17Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227937
HGNC: HGNC:9026
Homologene: 2689

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Mta1
Name: metastasis associated 1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 116870
HGNC: HGNC:7410
Homologene: 3442

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
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ClinGen
Dtnb
Name: dystrobrevin, beta
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 13528
HGNC: HGNC:3058
Homologene: 74875

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Arid1a
Name: AT-rich interaction domain 1A
Synonyms: Osa1, 1110030E03Rik, Smarcf1, BAF250a
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 93760
Homologene: 21216

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Knl1
Name: kinetochore scaffold 1
Synonyms: 2310043D08Rik, 5730505K17Rik, Casc5
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 76464
Homologene: 44890

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 64,937,984 bp
  • T to A, chromosome 1 at 74,633,566 bp
  • T to C, chromosome 1 at 82,882,309 bp
  • A to T, chromosome 1 at 188,733,529 bp
  • C to T, chromosome 2 at 30,404,565 bp
  • G to A, chromosome 2 at 37,852,172 bp
  • T to C, chromosome 2 at 59,311,766 bp
  • T to A, chromosome 2 at 87,490,845 bp
  • T to A, chromosome 2 at 90,149,844 bp
  • A to G, chromosome 2 at 119,070,592 bp
  • A to G, chromosome 2 at 121,138,704 bp
  • T to A, chromosome 2 at 146,222,791 bp
  • T to A, chromosome 2 at 147,042,097 bp
  • T to A, chromosome 3 at 94,763,055 bp
  • C to T, chromosome 3 at 95,007,530 bp
  • A to G, chromosome 3 at 109,853,082 bp
  • A to G, chromosome 3 at 153,876,745 bp
  • A to T, chromosome 4 at 52,612,108 bp
  • A to G, chromosome 4 at 101,175,182 bp
  • C to T, chromosome 4 at 118,689,662 bp
  • G to T, chromosome 4 at 133,681,080 bp
  • T to C, chromosome 4 at 156,233,961 bp
  • A to G, chromosome 5 at 3,630,222 bp
  • A to T, chromosome 5 at 7,978,453 bp
  • T to C, chromosome 5 at 45,695,793 bp
  • A to C, chromosome 5 at 117,867,518 bp
  • T to A, chromosome 5 at 135,852,206 bp
  • A to C, chromosome 6 at 29,470,195 bp
  • C to T, chromosome 6 at 81,965,812 bp
  • A to G, chromosome 7 at 49,759,786 bp
  • G to A, chromosome 7 at 80,378,662 bp
  • A to G, chromosome 7 at 107,106,067 bp
  • G to A, chromosome 8 at 12,661,359 bp
  • A to T, chromosome 8 at 117,028,479 bp
  • A to T, chromosome 9 at 32,245,924 bp
  • T to C, chromosome 9 at 37,410,981 bp
  • T to C, chromosome 9 at 53,569,916 bp
  • T to A, chromosome 9 at 101,102,117 bp
  • T to C, chromosome 9 at 114,631,826 bp
  • T to C, chromosome 10 at 116,937,641 bp
  • T to C, chromosome 10 at 121,689,834 bp
  • A to G, chromosome 10 at 129,570,624 bp
  • A to G, chromosome 11 at 77,953,530 bp
  • T to A, chromosome 12 at 3,644,272 bp
  • T to C, chromosome 12 at 29,990,945 bp
  • T to G, chromosome 12 at 111,633,789 bp
  • G to A, chromosome 12 at 113,131,468 bp
  • T to C, chromosome 13 at 21,467,843 bp
  • T to C, chromosome 13 at 54,789,653 bp
  • A to G, chromosome 13 at 74,739,841 bp
  • G to A, chromosome 13 at 100,629,511 bp
  • T to C, chromosome 14 at 30,943,160 bp
  • A to G, chromosome 14 at 33,078,307 bp
  • A to G, chromosome 14 at 47,289,839 bp
  • A to G, chromosome 14 at 50,642,395 bp
  • A to C, chromosome 14 at 78,982,234 bp
  • T to C, chromosome 14 at 101,880,953 bp
  • T to A, chromosome 14 at 123,291,860 bp
  • A to G, chromosome 15 at 3,347,572 bp
  • A to G, chromosome 15 at 63,804,466 bp
  • A to G, chromosome 15 at 75,866,353 bp
  • A to T, chromosome 15 at 76,332,993 bp
  • T to A, chromosome 16 at 16,261,673 bp
  • C to A, chromosome 16 at 32,986,743 bp
  • A to G, chromosome 16 at 36,898,547 bp
  • T to C, chromosome 18 at 35,213,635 bp
  • T to G, chromosome 18 at 82,393,865 bp
  • T to A, chromosome 19 at 21,428,624 bp
  • T to C, chromosome 19 at 31,083,193 bp
  • A to G, chromosome 19 at 40,738,865 bp
  • A to T, chromosome 19 at 56,808,178 bp

Strain Description

Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.

Strain Origin

Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7408 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
Black

Breeding

MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain

Reproductive Statistics

Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
045489-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.