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Strain Name:
C57BL/6J-MtgxR7417Btlr/Mmmh
Stock Number:
045495-MU
Citation ID:
RRID:MMRRC_045495-MU
Other Names:
R7417 (G1)
Major Collection:

Strain Information

Myh9
Name: myosin, heavy polypeptide 9, non-muscle
Synonyms: D0Jmb2, E030044M24Rik, NMHC II-A, Myhn-1, Myhn1, myosin IIA, Fltn
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 17886
HGNC: HGNC:7579
Homologene: 129835
Ptprf
Name: protein tyrosine phosphatase receptor type F
Synonyms: LAR, RPTP-LAR
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 19268
HGNC: HGNC:9670
Homologene: 20623
Prdm2
Name: PR domain containing 2, with ZNF domain
Synonyms: Riz, Riz1, LOC381568, E330024L24Rik, 4833427P12Rik, KMT8
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 110593
HGNC: HGNC:9347
Homologene: 40822
Top3b
Name: topoisomerase (DNA) III beta
Synonyms: Topo III beta
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 21976
Homologene: 2923
Nln
Name: neurolysin (metallopeptidase M3 family)
Synonyms: 4930472G13Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 75805
VEGA: 13
Homologene: 69315
Swap70
Name: SWA-70 protein
Synonyms: 70kDa
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 20947
Homologene: 7557
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 9,746,315 bp
  • T to C, chromosome 1 at 53,197,072 bp
  • A to G, chromosome 1 at 183,327,653 bp
  • T to C, chromosome 2 at 13,426,967 bp
  • A to T, chromosome 2 at 26,421,397 bp
  • A to G, chromosome 2 at 30,102,822 bp
  • A to C, chromosome 2 at 112,072,100 bp
  • A to C, chromosome 2 at 121,440,410 bp
  • A to T, chromosome 2 at 122,787,530 bp
  • A to T, chromosome 2 at 151,600,343 bp
  • A to G, chromosome 3 at 27,098,419 bp
  • A to G, chromosome 3 at 95,125,863 bp
  • A to G, chromosome 3 at 106,768,919 bp
  • A to G, chromosome 3 at 123,671,664 bp
  • A to T, chromosome 3 at 146,093,080 bp
  • A to G, chromosome 4 at 34,595,136 bp
  • A to G, chromosome 4 at 94,811,345 bp
  • C to T, chromosome 4 at 96,201,988 bp
  • T to C, chromosome 4 at 105,075,543 bp
  • T to C, chromosome 4 at 118,212,172 bp
  • G to A, chromosome 4 at 121,054,292 bp
  • A to G, chromosome 4 at 124,825,181 bp
  • T to C, chromosome 4 at 132,117,452 bp
  • T to A, chromosome 4 at 133,248,396 bp
  • A to T, chromosome 4 at 143,179,299 bp
  • A to G, chromosome 4 at 148,605,721 bp
  • G to A, chromosome 6 at 34,417,365 bp
  • G to A, chromosome 6 at 82,743,345 bp
  • A to T, chromosome 6 at 83,332,510 bp
  • T to A, chromosome 6 at 89,933,053 bp
  • A to G, chromosome 7 at 12,041,684 bp
  • A to G, chromosome 7 at 18,722,966 bp
  • A to T, chromosome 7 at 19,788,974 bp
  • A to T, chromosome 7 at 45,684,725 bp
  • A to C, chromosome 7 at 67,733,206 bp
  • A to T, chromosome 7 at 98,615,486 bp
  • T to A, chromosome 7 at 110,264,109 bp
  • G to T, chromosome 7 at 143,070,688 bp
  • A to T, chromosome 8 at 22,062,028 bp
  • T to C, chromosome 8 at 48,236,183 bp
  • T to A, chromosome 8 at 71,480,833 bp
  • C to T, chromosome 8 at 85,009,666 bp
  • T to A, chromosome 8 at 111,273,069 bp
  • T to C, chromosome 8 at 117,662,751 bp
  • T to C, chromosome 8 at 125,482,106 bp
  • T to C, chromosome 9 at 20,500,936 bp
  • G to A, chromosome 9 at 37,523,294 bp
  • A to C, chromosome 9 at 44,956,713 bp
  • T to A, chromosome 10 at 7,774,080 bp
  • T to A, chromosome 11 at 6,593,091 bp
  • T to C, chromosome 11 at 22,974,662 bp
  • G to T, chromosome 11 at 59,129,577 bp
  • A to G, chromosome 11 at 60,454,680 bp
  • A to G, chromosome 11 at 77,476,194 bp
  • A to G, chromosome 11 at 101,524,981 bp
  • A to T, chromosome 11 at 116,852,901 bp
  • A to G, chromosome 12 at 28,866,955 bp
  • T to C, chromosome 12 at 84,441,782 bp
  • A to T, chromosome 12 at 103,088,758 bp
  • A to C, chromosome 13 at 11,556,748 bp
  • A to G, chromosome 13 at 24,696,550 bp
  • A to G, chromosome 13 at 99,971,777 bp
  • A to G, chromosome 13 at 104,036,970 bp
  • T to A, chromosome 13 at 109,632,788 bp
  • A to T, chromosome 14 at 14,349,345 bp
  • T to C, chromosome 14 at 31,370,706 bp
  • T to A, chromosome 15 at 77,763,865 bp
  • T to A, chromosome 15 at 85,097,542 bp
  • G to A, chromosome 16 at 16,877,850 bp
  • C to A, chromosome 16 at 45,619,877 bp
  • T to A, chromosome 17 at 33,847,556 bp
  • T to A, chromosome 17 at 38,335,292 bp
  • G to A, chromosome 17 at 48,152,889 bp
  • A to G, chromosome 17 at 74,446,488 bp
  • A to G, chromosome 17 at 86,136,321 bp
  • T to C, chromosome 19 at 24,900,462 bp
  • A to T, chromosome 19 at 25,678,598 bp
  • G to A, chromosome 19 at 45,010,564 bp
  • C to T, chromosome X at 8,962,804 bp
  • C to T, chromosome X at 8,962,808 bp
  • G to A, chromosome X at 136,927,722 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7417 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045495-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.