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Strain Name:
C57BL/6J-MtgxR7418Btlr/Mmmh
Stock Number:
045496-MU
Citation ID:
RRID:MMRRC_045496-MU
Other Names:
R7418 (G1)
Major Collection:

Strain Information

Trp53
Name: transformation related protein 53
Synonyms: p53, p44
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 22059
Homologene: 460
Sphk2
Name: sphingosine kinase 2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 56632
Homologene: 32456
Sbf2
Name: SET binding factor 2
Synonyms: SBF2, 4833411B01Rik, mMTMH1, B430219L04Rik, Mtmr13
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 319934
HGNC: HGNC:2135
Homologene: 41810
Inpp5d
Name: inositol polyphosphate-5-phosphatase D
Synonyms: SHIP, Src homology 2 domain-containing inositol-5-phosphatase, s-SHIP, SHIP-1, SHIP1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 16331
HGNC: HGNC:6079
Homologene: 4046
Npl
Name: N-acetylneuraminate pyruvate lyase
Synonyms: 0610033B02Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 74091
Homologene: 12533
Slit3
Name: slit guidance ligand 3
Synonyms: Slit1, b2b2362.1Clo
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20564
Homologene: 2303
Kat2b
Name: K(lysine) acetyltransferase 2B
Synonyms: A930006P13Rik, Pcaf
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 18519
HGNC: HGNC:8638
Homologene: 20834
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 59,526,813 bp
  • T to A, chromosome 1 at 66,926,179 bp
  • T to A, chromosome 1 at 87,708,211 bp
  • T to A, chromosome 1 at 91,507,736 bp
  • A to T, chromosome 1 at 153,537,511 bp
  • A to G, chromosome 1 at 155,710,259 bp
  • C to T, chromosome 1 at 181,616,742 bp
  • C to T, chromosome 2 at 24,884,634 bp
  • A to G, chromosome 2 at 32,452,571 bp
  • C to T, chromosome 2 at 34,725,118 bp
  • A to G, chromosome 2 at 76,772,447 bp
  • A to T, chromosome 2 at 89,788,976 bp
  • T to G, chromosome 2 at 90,149,487 bp
  • C to T, chromosome 3 at 95,240,567 bp
  • C to A, chromosome 3 at 107,195,046 bp
  • C to T, chromosome 3 at 125,708,151 bp
  • T to C, chromosome 4 at 45,904,616 bp
  • T to C, chromosome 4 at 48,051,476 bp
  • A to G, chromosome 4 at 86,594,773 bp
  • A to G, chromosome 4 at 118,160,243 bp
  • A to G, chromosome 4 at 132,680,848 bp
  • T to A, chromosome 5 at 21,581,983 bp
  • A to G, chromosome 5 at 21,760,624 bp
  • G to A, chromosome 5 at 30,622,331 bp
  • A to T, chromosome 5 at 31,252,537 bp
  • T to C, chromosome 5 at 34,790,353 bp
  • C to T, chromosome 5 at 101,957,500 bp
  • T to A, chromosome 5 at 124,576,830 bp
  • C to T, chromosome 6 at 55,679,298 bp
  • T to C, chromosome 6 at 92,024,538 bp
  • G to A, chromosome 6 at 135,217,011 bp
  • A to G, chromosome 7 at 5,803,555 bp
  • A to T, chromosome 7 at 11,847,154 bp
  • G to A, chromosome 7 at 45,711,756 bp
  • G to A, chromosome 7 at 79,742,987 bp
  • G to A, chromosome 7 at 97,490,972 bp
  • G to T, chromosome 7 at 101,271,624 bp
  • C to T, chromosome 7 at 110,365,821 bp
  • A to G, chromosome 8 at 72,196,794 bp
  • G to T, chromosome 8 at 95,278,259 bp
  • A to G, chromosome 8 at 106,688,834 bp
  • G to A, chromosome 8 at 119,312,525 bp
  • A to G, chromosome 9 at 55,544,352 bp
  • C to T, chromosome 9 at 106,022,915 bp
  • A to T, chromosome 9 at 119,959,051 bp
  • A to T, chromosome 10 at 80,530,092 bp
  • T to G, chromosome 10 at 91,023,835 bp
  • G to A, chromosome 11 at 35,686,428 bp
  • T to C, chromosome 11 at 69,588,388 bp
  • A to T, chromosome 11 at 115,498,269 bp
  • C to A, chromosome 11 at 121,069,293 bp
  • A to T, chromosome 12 at 31,833,241 bp
  • G to A, chromosome 12 at 58,268,647 bp
  • A to G, chromosome 13 at 21,484,629 bp
  • A to G, chromosome 15 at 66,696,583 bp
  • A to G, chromosome 15 at 98,531,894 bp
  • T to G, chromosome 16 at 33,885,094 bp
  • G to T, chromosome 16 at 49,155,537 bp
  • A to T, chromosome 16 at 77,113,842 bp
  • T to A, chromosome 17 at 6,084,628 bp
  • T to A, chromosome 17 at 26,253,182 bp
  • T to A, chromosome 17 at 29,140,496 bp
  • T to A, chromosome 17 at 33,910,605 bp
  • G to T, chromosome 17 at 47,672,168 bp
  • G to T, chromosome 17 at 53,610,925 bp
  • A to G, chromosome 17 at 73,913,965 bp
  • C to T, chromosome 19 at 24,280,496 bp
  • T to C, chromosome 19 at 34,929,687 bp
  • C to T, chromosome X at 8,962,804 bp
  • C to T, chromosome X at 8,962,808 bp
  • G to A, chromosome X at 136,927,722 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7418 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045496-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.