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Strain Detail Sheet

Strain Name:
C57BL/6J-MtgxR7422Btlr/Mmmh
Stock Number:
045500-MU
Citation ID:
RRID:MMRRC_045500-MU
Other Names:
R7422 (G1)
Major Collection:
Beutler Mutagenetix

Strain Information

Gene Details

Arhgef7
Name: Rho guanine nucleotide exchange factor
Synonyms: Cool, PIX, Pak interacting exchange factor, p85SPR, betaPix-c, betaPix-b, cool-1, betaPix
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 54126
Homologene: 2895

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KOMP Phenotyping
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Ints6
Name: integrator complex subunit 6
Synonyms: Notch2l, 2900075H24Rik, DICE1, Ddx26
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 18130
VEGA: 14
Homologene: 8121

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IMPC
Google
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Gene Ontology
BioGPS
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Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
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Tanc1
Name: tetratricopeptide repeat, ankyrin repeat and coiled-coil containing 1
Synonyms: 1200003E16Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 66860
Homologene: 18946

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Google
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Por
Name: cytochrome p450 oxidoreductase
Synonyms: NADH cytochrome P450 oxydoreductase, CYPOR, CPR, 4933424M13Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 18984
HGNC: HGNC:9208
Homologene: 725

Additional Resources
IMPC
Google
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IMSR
Gene Ontology
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Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
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ClinGen
Qtrt1
Name: queuine tRNA-ribosyltransferase catalytic subunit 1
Synonyms: Tgt, tRNA-guanine transglycosylase, 2610028E17Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 60507
VEGA: 9
Homologene: 11061

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Dock5
Name: dedicator of cytokinesis 5
Synonyms: lr2, 1110060D06Rik, rlc
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 68813
VEGA: 14
Homologene: 57016

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Smc2
Name: structural maintenance of chromosomes 2
Synonyms: 5730502P04Rik, CAP-E, Fin16, Smc2l1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 14211
Homologene: 4705

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to C, chromosome 1 at 83,263,826 bp
  • A to G, chromosome 1 at 87,149,612 bp
  • A to T, chromosome 1 at 97,081,482 bp
  • A to G, chromosome 1 at 149,932,687 bp
  • A to G, chromosome 2 at 29,872,880 bp
  • T to C, chromosome 2 at 59,806,344 bp
  • T to C, chromosome 2 at 69,953,542 bp
  • T to A, chromosome 2 at 76,593,386 bp
  • T to A, chromosome 2 at 89,326,079 bp
  • C to A, chromosome 2 at 120,702,152 bp
  • C to T, chromosome 2 at 137,085,055 bp
  • A to G, chromosome 2 at 163,321,515 bp
  • A to T, chromosome 2 at 168,648,593 bp
  • T to C, chromosome 2 at 174,457,032 bp
  • C to T, chromosome 2 at 178,394,151 bp
  • C to T, chromosome 3 at 34,049,220 bp
  • T to A, chromosome 3 at 89,366,686 bp
  • T to A, chromosome 3 at 89,854,557 bp
  • T to C, chromosome 4 at 42,773,906 bp
  • C to A, chromosome 4 at 52,440,301 bp
  • T to A, chromosome 4 at 96,140,985 bp
  • T to A, chromosome 5 at 31,731,049 bp
  • C to T, chromosome 5 at 96,673,599 bp
  • T to C, chromosome 5 at 99,979,209 bp
  • T to A, chromosome 5 at 108,731,689 bp
  • T to A, chromosome 5 at 110,162,705 bp
  • T to C, chromosome 5 at 120,479,189 bp
  • A to T, chromosome 5 at 135,734,919 bp
  • A to G, chromosome 5 at 143,090,836 bp
  • A to T, chromosome 6 at 24,875,984 bp
  • G to T, chromosome 6 at 29,455,471 bp
  • A to G, chromosome 6 at 42,538,119 bp
  • T to A, chromosome 7 at 6,394,749 bp
  • T to C, chromosome 7 at 24,916,036 bp
  • G to T, chromosome 7 at 29,085,870 bp
  • A to T, chromosome 7 at 31,375,837 bp
  • T to C, chromosome 7 at 35,428,247 bp
  • T to G, chromosome 7 at 82,681,379 bp
  • A to C, chromosome 7 at 84,946,361 bp
  • T to A, chromosome 7 at 98,051,626 bp
  • A to G, chromosome 7 at 108,124,861 bp
  • G to T, chromosome 7 at 130,533,817 bp
  • G to A, chromosome 7 at 135,698,370 bp
  • T to A, chromosome 8 at 11,800,861 bp
  • T to C, chromosome 8 at 13,222,873 bp
  • A to T, chromosome 8 at 56,569,282 bp
  • A to T, chromosome 8 at 70,756,523 bp
  • A to C, chromosome 8 at 80,615,763 bp
  • T to G, chromosome 8 at 85,355,244 bp
  • A to G, chromosome 8 at 122,336,959 bp
  • A to T, chromosome 9 at 21,412,457 bp
  • T to A, chromosome 9 at 53,912,843 bp
  • T to C, chromosome 9 at 54,954,882 bp
  • A to G, chromosome 9 at 80,113,877 bp
  • A to G, chromosome 9 at 123,607,617 bp
  • T to C, chromosome 10 at 21,389,097 bp
  • T to C, chromosome 10 at 24,076,864 bp
  • G to T, chromosome 10 at 76,603,336 bp
  • A to G, chromosome 10 at 120,108,894 bp
  • A to T, chromosome 10 at 129,768,267 bp
  • C to A, chromosome 11 at 4,738,856 bp
  • T to A, chromosome 11 at 58,881,059 bp
  • T to A, chromosome 11 at 75,759,343 bp
  • A to G, chromosome 11 at 94,968,358 bp
  • G to T, chromosome 11 at 96,292,684 bp
  • T to C, chromosome 11 at 102,438,665 bp
  • A to T, chromosome 11 at 105,292,783 bp
  • C to T, chromosome 11 at 107,060,558 bp
  • A to G, chromosome 12 at 9,004,105 bp
  • G to T, chromosome 12 at 11,326,438 bp
  • A to G, chromosome 12 at 76,406,110 bp
  • A to G, chromosome 12 at 101,588,034 bp
  • G to A, chromosome 13 at 8,757,277 bp
  • G to T, chromosome 13 at 35,858,194 bp
  • G to A, chromosome 13 at 54,378,850 bp
  • A to T, chromosome 13 at 55,351,525 bp
  • A to C, chromosome 13 at 73,954,578 bp
  • A to T, chromosome 13 at 74,150,885 bp
  • G to A, chromosome 13 at 94,528,165 bp
  • A to T, chromosome 14 at 62,704,775 bp
  • A to G, chromosome 14 at 63,142,303 bp
  • C to T, chromosome 14 at 65,032,298 bp
  • T to A, chromosome 14 at 67,809,030 bp
  • T to G, chromosome 14 at 68,626,877 bp
  • T to C, chromosome 15 at 5,012,056 bp
  • A to T, chromosome 15 at 77,714,352 bp
  • T to A, chromosome 15 at 80,160,244 bp
  • T to C, chromosome 15 at 83,306,949 bp
  • T to C, chromosome 16 at 16,318,474 bp
  • T to A, chromosome 16 at 32,754,689 bp
  • T to A, chromosome 17 at 20,502,797 bp
  • T to C, chromosome 17 at 35,203,829 bp
  • T to A, chromosome 17 at 35,638,420 bp
  • T to A, chromosome 17 at 58,410,231 bp
  • C to A, chromosome 19 at 3,899,008 bp
  • A to G, chromosome 19 at 3,966,476 bp
  • A to C, chromosome 19 at 10,871,760 bp
  • A to T, chromosome 19 at 16,750,173 bp
  • T to A, chromosome 19 at 37,174,500 bp
  • T to C, chromosome 19 at 38,651,885 bp

Strain Description

Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.

Strain Origin

Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7422 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
Black

Breeding

MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain

Reproductive Statistics

Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
045500-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.