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Strain Detail Sheet

Strain Name:
C57BL/6J-MtgxR7432Btlr/Mmmh
Stock Number:
045510-MU
Citation ID:
RRID:MMRRC_045510-MU
Other Names:
R7432 (G1)
Major Collection:
Beutler Mutagenetix

Strain Information

Gene Details

Slc12a1
Name: solute carrier family 12, member 1
Synonyms: Nkcc2, mBSC1, D630042G03Rik, urehr3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20495
Homologene: 286

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Gene Ontology
BioGPS
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KOMP Phenotyping
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ClinGen
Pip5k1a
Name: phosphatidylinositol-4-phosphate 5-kinase, type 1 alpha
Synonyms: Pipk5a
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18720
HGNC: HGNC:8994
Homologene: 93492

Additional Resources
IMPC
Google
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IMSR
Gene Ontology
BioGPS
Gene Cloud
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Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
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ClinGen
Pdpk1
Name: 3-phosphoinositide dependent protein kinase 1
Synonyms: Pkb kinase, Pdk1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 18607
Homologene: 37643

Additional Resources
IMPC
Google
Entrez
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IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
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ClinGen
Sema3e
Name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E
Synonyms: Semah
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 20349
Homologene: 8247

Additional Resources
IMPC
Google
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IMSR
Gene Ontology
BioGPS
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Mouse Mine
KOMP Phenotyping
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Ppm1a
Name: protein phosphatase 1A, magnesium dependent, alpha isoform
Synonyms: Mpp alpha, MMPa-2, MPPa-1, 2900017D14Rik, 2310003C21Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 19042
VEGA: 12
HGNC: HGNC:9275
Homologene: 56428

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Rgs11
Name: regulator of G-protein signaling 11
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 50782
HGNC: HGNC:9993
Homologene: 77719

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Nsd1
Name: nuclear receptor-binding SET-domain protein 1
Synonyms: KMT3B
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 18193
VEGA: 13
Homologene: 32543

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 92,896,079 bp
  • C to T, chromosome 1 at 131,830,956 bp
  • T to C, chromosome 1 at 143,765,810 bp
  • T to A, chromosome 1 at 154,205,037 bp
  • A to T, chromosome 1 at 156,302,432 bp
  • T to A, chromosome 1 at 170,879,776 bp
  • C to A, chromosome 1 at 192,137,876 bp
  • T to C, chromosome 2 at 25,609,457 bp
  • A to G, chromosome 2 at 76,766,287 bp
  • G to T, chromosome 2 at 86,786,221 bp
  • T to C, chromosome 2 at 87,532,440 bp
  • A to T, chromosome 2 at 90,037,184 bp
  • A to G, chromosome 2 at 111,692,056 bp
  • T to C, chromosome 2 at 117,287,943 bp
  • A to T, chromosome 2 at 120,722,755 bp
  • G to T, chromosome 2 at 125,206,040 bp
  • A to G, chromosome 2 at 144,317,872 bp
  • G to T, chromosome 2 at 146,434,856 bp
  • T to C, chromosome 2 at 181,589,856 bp
  • A to G, chromosome 3 at 88,051,696 bp
  • T to G, chromosome 3 at 95,074,120 bp
  • T to A, chromosome 3 at 97,695,092 bp
  • C to T, chromosome 3 at 109,003,407 bp
  • T to C, chromosome 4 at 43,647,155 bp
  • T to A, chromosome 4 at 44,032,419 bp
  • C to G, chromosome 4 at 45,399,765 bp
  • C to T, chromosome 4 at 56,776,925 bp
  • T to C, chromosome 4 at 99,753,058 bp
  • T to A, chromosome 4 at 105,794,289 bp
  • T to C, chromosome 4 at 120,529,156 bp
  • T to C, chromosome 4 at 139,388,382 bp
  • T to G, chromosome 5 at 14,224,390 bp
  • T to A, chromosome 5 at 24,367,615 bp
  • T to G, chromosome 5 at 86,496,507 bp
  • C to G, chromosome 5 at 116,024,162 bp
  • T to G, chromosome 5 at 118,751,938 bp
  • G to A, chromosome 5 at 121,537,171 bp
  • T to C, chromosome 5 at 123,991,766 bp
  • C to A, chromosome 6 at 40,572,306 bp
  • G to T, chromosome 6 at 64,275,870 bp
  • T to C, chromosome 6 at 113,462,371 bp
  • A to C, chromosome 7 at 3,222,539 bp
  • G to A, chromosome 7 at 25,005,875 bp
  • C to T, chromosome 7 at 29,895,359 bp
  • C to A, chromosome 7 at 41,426,724 bp
  • A to G, chromosome 7 at 67,051,917 bp
  • A to T, chromosome 7 at 126,493,874 bp
  • A to G, chromosome 7 at 127,023,048 bp
  • G to T, chromosome 8 at 22,773,651 bp
  • A to G, chromosome 8 at 71,996,062 bp
  • A to G, chromosome 8 at 80,788,669 bp
  • T to C, chromosome 9 at 21,343,215 bp
  • T to A, chromosome 9 at 46,287,074 bp
  • A to G, chromosome 9 at 78,714,943 bp
  • T to C, chromosome 9 at 122,157,210 bp
  • C to A, chromosome 10 at 4,432,706 bp
  • C to A, chromosome 10 at 68,118,266 bp
  • T to C, chromosome 10 at 86,885,683 bp
  • C to T, chromosome 10 at 88,798,398 bp
  • T to A, chromosome 10 at 128,016,016 bp
  • T to C, chromosome 10 at 129,873,850 bp
  • C to A, chromosome 11 at 4,213,016 bp
  • T to C, chromosome 11 at 23,518,839 bp
  • T to C, chromosome 11 at 36,864,941 bp
  • C to T, chromosome 11 at 59,028,907 bp
  • A to G, chromosome 11 at 65,814,363 bp
  • T to A, chromosome 11 at 70,437,970 bp
  • A to G, chromosome 11 at 98,851,114 bp
  • T to C, chromosome 12 at 4,850,246 bp
  • A to T, chromosome 12 at 55,398,828 bp
  • C to T, chromosome 12 at 72,784,142 bp
  • T to A, chromosome 12 at 73,968,294 bp
  • A to T, chromosome 12 at 105,661,204 bp
  • A to T, chromosome 12 at 105,664,698 bp
  • G to A, chromosome 13 at 4,088,952 bp
  • A to T, chromosome 13 at 40,887,212 bp
  • A to G, chromosome 13 at 55,213,374 bp
  • T to C, chromosome 14 at 14,350,691 bp
  • C to A, chromosome 14 at 33,507,553 bp
  • T to A, chromosome 14 at 54,180,577 bp
  • C to A, chromosome 14 at 101,902,115 bp
  • T to C, chromosome 16 at 10,688,555 bp
  • T to A, chromosome 16 at 20,654,925 bp
  • T to C, chromosome 16 at 33,089,368 bp
  • C to T, chromosome 16 at 84,857,520 bp
  • C to T, chromosome 16 at 89,419,873 bp
  • T to C, chromosome 17 at 24,101,669 bp
  • G to A, chromosome 17 at 26,207,760 bp
  • T to C, chromosome 17 at 26,682,698 bp
  • T to C, chromosome 17 at 32,798,759 bp
  • A to G, chromosome 17 at 37,481,470 bp
  • T to C, chromosome 17 at 46,255,783 bp
  • G to T, chromosome 18 at 20,446,266 bp
  • A to G, chromosome 18 at 25,001,909 bp
  • C to A, chromosome 18 at 36,962,326 bp
  • T to C, chromosome 18 at 42,054,026 bp
  • G to T, chromosome 18 at 77,295,851 bp
  • A to G, chromosome 18 at 80,765,841 bp
  • G to A, chromosome 19 at 40,959,857 bp
  • A to T, chromosome 19 at 43,468,271 bp
  • A to C, chromosome 19 at 57,755,524 bp

Strain Description

Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.

Strain Origin

Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7432 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
Black

Breeding

MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain

Reproductive Statistics

Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
045510-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.