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Strain Name:
C57BL/6J-MtgxR7452Btlr/Mmmh
Stock Number:
045526-MU
Citation ID:
RRID:MMRRC_045526-MU
Other Names:
R7452 (G1)
Major Collection:

Strain Information

Chd7
Name: chromodomain helicase DNA binding protein 7
Synonyms: GENA 60, GENA 47, Gena 52, Cyn, A730019I05Rik, WBE1, Whi, Todo, Obt, Mt, Lda, Flo, Edy, Dz, Cycn
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 320790
Homologene: 19067
Iqgap1
Name: IQ motif containing GTPase activating protein 1
Synonyms: D7Ertd237e, D7Ertd257e
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 29875
HGNC: HGNC:6110
Homologene: 74514
Kdm5b
Name: lysine demethylase 5B
Synonyms: PLU-1, 2010009J12Rik, Plu1, Rb-Bp2, 2210016I17Rik, D1Ertd202e, Jarid1b
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 75605
Homologene: 48448
Rictor
Name: RPTOR independent companion of MTOR, complex 2
Synonyms: 6030405M08Rik, D530039E11Rik, 4921505C17Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 78757
VEGA: 15
Homologene: 34317
Map1b
Name: microtubule-associated protein 1B
Synonyms: MAP5, Mtap-5, Mtap5, LC1, Mtap1b
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 17755
VEGA: 13
HGNC: HGNC:6836
Homologene: 38111
Poli
Name: polymerase (DNA directed), iota
Synonyms: Rad30b
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 26447
HGNC: HGNC:9182
Homologene: 5209
Fryl
Name: FRY like transcription coactivator
Synonyms: 2310004H21Rik, 2510002A14Rik, 9030227G01Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 72313
Homologene: 103956
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 13,125,559 bp
  • A to T, chromosome 1 at 46,647,036 bp
  • A to G, chromosome 1 at 86,213,092 bp
  • T to A, chromosome 1 at 93,872,518 bp
  • T to C, chromosome 1 at 120,227,855 bp
  • T to C, chromosome 1 at 134,624,948 bp
  • C to T, chromosome 1 at 182,446,568 bp
  • T to A, chromosome 1 at 191,999,831 bp
  • C to A, chromosome 1 at 192,137,876 bp
  • A to G, chromosome 2 at 10,238,796 bp
  • A to G, chromosome 2 at 58,827,584 bp
  • A to G, chromosome 2 at 76,136,414 bp
  • G to T, chromosome 2 at 76,877,143 bp
  • A to G, chromosome 2 at 111,306,921 bp
  • T to C, chromosome 2 at 125,505,455 bp
  • A to T, chromosome 2 at 165,427,114 bp
  • A to T, chromosome 3 at 20,011,428 bp
  • C to T, chromosome 3 at 33,004,318 bp
  • C to T, chromosome 3 at 59,116,045 bp
  • T to C, chromosome 3 at 68,994,282 bp
  • T to C, chromosome 3 at 82,864,121 bp
  • T to A, chromosome 3 at 108,413,090 bp
  • T to A, chromosome 3 at 129,271,403 bp
  • A to G, chromosome 3 at 138,454,745 bp
  • A to C, chromosome 4 at 8,854,731 bp
  • T to C, chromosome 4 at 32,739,030 bp
  • A to T, chromosome 4 at 115,327,598 bp
  • T to C, chromosome 4 at 118,595,784 bp
  • G to A, chromosome 4 at 121,417,474 bp
  • A to C, chromosome 4 at 133,753,127 bp
  • A to G, chromosome 5 at 30,893,391 bp
  • A to T, chromosome 5 at 62,676,549 bp
  • T to A, chromosome 5 at 72,435,247 bp
  • A to G, chromosome 5 at 73,023,988 bp
  • A to G, chromosome 5 at 92,392,680 bp
  • A to G, chromosome 5 at 125,638,268 bp
  • A to G, chromosome 6 at 17,282,076 bp
  • T to G, chromosome 6 at 48,908,790 bp
  • C to A, chromosome 6 at 121,641,332 bp
  • A to G, chromosome 7 at 34,245,671 bp
  • T to A, chromosome 7 at 49,773,326 bp
  • T to A, chromosome 7 at 75,147,713 bp
  • A to G, chromosome 7 at 80,760,829 bp
  • C to T, chromosome 7 at 108,508,593 bp
  • C to T, chromosome 8 at 13,035,215 bp
  • T to C, chromosome 8 at 19,225,524 bp
  • A to G, chromosome 8 at 70,177,488 bp
  • A to T, chromosome 9 at 38,607,088 bp
  • C to T, chromosome 9 at 44,279,600 bp
  • T to C, chromosome 9 at 66,566,898 bp
  • A to G, chromosome 9 at 106,989,465 bp
  • T to C, chromosome 10 at 41,240,637 bp
  • A to G, chromosome 10 at 69,899,051 bp
  • C to A, chromosome 11 at 49,524,381 bp
  • T to C, chromosome 12 at 36,212,693 bp
  • T to C, chromosome 12 at 58,213,356 bp
  • A to T, chromosome 12 at 69,337,959 bp
  • T to C, chromosome 12 at 80,183,602 bp
  • C to T, chromosome 13 at 99,508,140 bp
  • A to C, chromosome 14 at 43,110,044 bp
  • A to G, chromosome 14 at 70,571,486 bp
  • C to T, chromosome 15 at 6,772,154 bp
  • T to A, chromosome 15 at 9,113,832 bp
  • T to C, chromosome 15 at 54,866,736 bp
  • T to A, chromosome 15 at 83,014,962 bp
  • T to C, chromosome 16 at 21,217,357 bp
  • G to T, chromosome 16 at 43,610,676 bp
  • T to C, chromosome 16 at 44,220,034 bp
  • T to A, chromosome 16 at 85,877,981 bp
  • T to A, chromosome 18 at 4,379,705 bp
  • A to G, chromosome 18 at 70,508,978 bp
  • A to T, chromosome 18 at 86,498,931 bp
  • T to C, chromosome 19 at 7,271,222 bp
  • T to A, chromosome 19 at 36,969,127 bp
  • G to A, chromosome X at 37,518,992 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7452 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045526-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.