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Strain Name:
C57BL/6J-MtgxR7466Btlr/Mmmh
Stock Number:
045540-MU
Citation ID:
RRID:MMRRC_045540-MU
Other Names:
R7466 (G1)
Major Collection:

Strain Information

Ippk
Name: inositol 1,3,4,5,6-pentakisphosphate 2-kinase
Synonyms: 1810043M15Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 75678
VEGA: 13
Homologene: 41495
Manba
Name: mannosidase, beta A, lysosomal
Synonyms: Bmn, 2410030O07Rik, B930014J03Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 110173
HGNC: HGNC:6831
Homologene: 4317
Dennd2b
Name: DENN domain containing 2B
Synonyms: 2610305K15Rik, 2010004M01Rik, St5, Denn2b
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 76954
Homologene: 3951
Prep
Name: prolyl endopeptidase
Synonyms: prolyl oligopeptidase, D10Wsu136e, Pop
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 19072
VEGA: 10
HGNC: HGNC:9358
Homologene: 2042
Grhl2
Name: grainyhead like transcription factor 2
Synonyms: BOM, 0610015A08Rik, Tcfcp2l3, grainyheadlike, clft3
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 252973
HGNC: HGNC:2799
Homologene: 32616
Phf20l1
Name: PHD finger protein 20-like 1
Synonyms: CGI-72, E130113K22Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 239510
VEGA: 15
Homologene: 9341
Rfc1
Name: replication factor C (activator 1) 1
Synonyms: RFC140, Alp145, 140kDa, Recc1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 19687
HGNC: HGNC:9969
Homologene: 2187
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 34,587,993 bp
  • A to T, chromosome 1 at 60,094,511 bp
  • C to T, chromosome 1 at 85,707,239 bp
  • A to T, chromosome 1 at 97,842,247 bp
  • C to T, chromosome 1 at 160,226,484 bp
  • C to T, chromosome 1 at 164,193,328 bp
  • C to T, chromosome 2 at 28,502,207 bp
  • G to T, chromosome 2 at 28,955,613 bp
  • T to C, chromosome 2 at 30,101,484 bp
  • T to A, chromosome 2 at 35,088,741 bp
  • T to C, chromosome 2 at 84,610,785 bp
  • T to C, chromosome 2 at 87,656,396 bp
  • T to C, chromosome 2 at 112,926,957 bp
  • C to A, chromosome 3 at 15,832,266 bp
  • T to A, chromosome 3 at 132,752,563 bp
  • C to A, chromosome 3 at 135,542,393 bp
  • T to C, chromosome 3 at 146,055,618 bp
  • T to A, chromosome 3 at 157,924,885 bp
  • T to A, chromosome 4 at 14,912,477 bp
  • A to G, chromosome 4 at 86,774,331 bp
  • C to T, chromosome 4 at 136,659,065 bp
  • A to G, chromosome 4 at 155,271,602 bp
  • G to A, chromosome 5 at 33,882,147 bp
  • A to T, chromosome 5 at 65,275,426 bp
  • T to A, chromosome 5 at 86,709,480 bp
  • A to G, chromosome 5 at 96,131,128 bp
  • T to C, chromosome 5 at 112,723,892 bp
  • A to T, chromosome 5 at 138,342,008 bp
  • T to A, chromosome 5 at 143,563,471 bp
  • T to C, chromosome 6 at 18,227,973 bp
  • A to G, chromosome 6 at 40,744,789 bp
  • G to T, chromosome 6 at 136,854,850 bp
  • A to C, chromosome 7 at 16,723,855 bp
  • C to T, chromosome 7 at 18,684,467 bp
  • A to C, chromosome 7 at 18,891,105 bp
  • G to A, chromosome 7 at 64,240,582 bp
  • A to T, chromosome 7 at 107,822,922 bp
  • A to G, chromosome 7 at 109,525,346 bp
  • T to A, chromosome 7 at 110,274,772 bp
  • A to G, chromosome 7 at 120,903,484 bp
  • A to T, chromosome 7 at 122,516,844 bp
  • T to C, chromosome 7 at 140,220,706 bp
  • A to C, chromosome 8 at 22,177,386 bp
  • AACGC to A, chromosome 8 at 56,540,983 bp
  • C to G, chromosome 9 at 64,928,404 bp
  • T to A, chromosome 9 at 71,163,261 bp
  • C to T, chromosome 9 at 79,655,407 bp
  • C to A, chromosome 9 at 106,196,157 bp
  • T to C, chromosome 9 at 120,955,416 bp
  • T to C, chromosome 10 at 45,150,438 bp
  • A to T, chromosome 11 at 46,817,758 bp
  • G to T, chromosome 11 at 55,310,432 bp
  • T to C, chromosome 11 at 65,828,531 bp
  • G to A, chromosome 11 at 69,784,650 bp
  • A to G, chromosome 11 at 69,804,885 bp
  • A to G, chromosome 11 at 107,492,057 bp
  • A to T, chromosome 11 at 119,374,502 bp
  • G to A, chromosome 12 at 9,005,773 bp
  • T to A, chromosome 12 at 76,046,186 bp
  • C to T, chromosome 12 at 79,287,807 bp
  • T to C, chromosome 12 at 80,640,983 bp
  • T to C, chromosome 13 at 4,192,437 bp
  • T to C, chromosome 13 at 33,495,167 bp
  • T to C, chromosome 13 at 49,432,467 bp
  • G to T, chromosome 13 at 96,082,779 bp
  • C to T, chromosome 13 at 97,131,220 bp
  • A to T, chromosome 13 at 100,221,986 bp
  • A to T, chromosome 13 at 102,704,995 bp
  • T to C, chromosome 14 at 21,986,011 bp
  • G to A, chromosome 14 at 24,245,212 bp
  • A to G, chromosome 15 at 37,291,616 bp
  • A to G, chromosome 15 at 66,636,884 bp
  • C to T, chromosome 15 at 76,648,186 bp
  • T to C, chromosome 15 at 98,313,380 bp
  • T to C, chromosome 16 at 31,447,604 bp
  • C to T, chromosome 16 at 44,909,174 bp
  • A to T, chromosome 17 at 32,883,762 bp
  • T to C, chromosome 17 at 36,120,849 bp
  • T to A, chromosome 17 at 46,639,910 bp
  • A to G, chromosome 17 at 50,608,468 bp
  • T to A, chromosome 17 at 88,635,901 bp
  • C to A, chromosome 19 at 8,914,569 bp
  • T to A, chromosome 19 at 11,870,316 bp
  • A to G, chromosome 19 at 52,264,420 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7466 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045540-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.