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Strain Name:
C57BL/6J-MtgxR7471Btlr/Mmmh
Stock Number:
045545-MU
Citation ID:
RRID:MMRRC_045545-MU
Other Names:
R7471 (G1)
Major Collection:

Strain Information

Sptbn4
Name: spectrin beta, non-erythrocytic 4
Synonyms: dyn, SpbIV, neuroaxonal dystrophy, 5830426A08Rik, ROSA62, nmf261, 1700022P15Rik, Spnb4
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 80297
Homologene: 11879
Dicer1
Name: dicer 1, ribonuclease type III
Synonyms: 1110006F08Rik, D12Ertd7e, Dicer1
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 192119
VEGA: 12
Homologene: 13251
Chd7
Name: chromodomain helicase DNA binding protein 7
Synonyms: GENA 60, GENA 47, Gena 52, Cyn, A730019I05Rik, WBE1, Whi, Todo, Obt, Mt, Lda, Flo, Edy, Dz, Cycn
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 320790
Homologene: 19067
Satb1
Name: special AT-rich sequence binding protein 1
Synonyms: 2610306G12Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 20230
Homologene: 2232
Inpp5d
Name: inositol polyphosphate-5-phosphatase D
Synonyms: SHIP, Src homology 2 domain-containing inositol-5-phosphatase, s-SHIP, SHIP-1, SHIP1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 16331
HGNC: HGNC:6079
Homologene: 4046
Dst
Name: dystonin
Synonyms: bullous pemphigoid antigen 1, BPAG1-n, BPAG1, Bpag1, Bpag, ah, bullous pemphigoid antigen 1, athetoid, Macf2, 2310001O04Rik, nmf203, A830042E19Rik, nmf339
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13518
HGNC: HGNC:1090
Homologene: 136716
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11426
Homologene: 136191
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 34,194,570 bp
  • A to G, chromosome 1 at 53,419,699 bp
  • T to C, chromosome 1 at 60,354,640 bp
  • A to T, chromosome 1 at 74,495,628 bp
  • A to G, chromosome 1 at 74,634,320 bp
  • A to G, chromosome 1 at 87,695,400 bp
  • G to A, chromosome 1 at 118,332,474 bp
  • A to T, chromosome 2 at 33,243,739 bp
  • T to A, chromosome 2 at 76,647,201 bp
  • T to C, chromosome 2 at 92,389,144 bp
  • C to T, chromosome 2 at 149,405,576 bp
  • T to A, chromosome 2 at 163,607,025 bp
  • A to G, chromosome 3 at 55,783,886 bp
  • A to T, chromosome 3 at 94,425,956 bp
  • T to A, chromosome 3 at 103,803,114 bp
  • A to G, chromosome 3 at 121,171,312 bp
  • A to T, chromosome 4 at 8,859,197 bp
  • A to G, chromosome 4 at 104,817,625 bp
  • A to T, chromosome 4 at 116,996,588 bp
  • A to T, chromosome 4 at 123,472,289 bp
  • A to T, chromosome 4 at 124,902,572 bp
  • A to G, chromosome 4 at 133,681,044 bp
  • A to G, chromosome 4 at 136,233,646 bp
  • A to T, chromosome 4 at 147,582,931 bp
  • T to A, chromosome 4 at 149,473,301 bp
  • C to G, chromosome 5 at 15,934,975 bp
  • C to T, chromosome 5 at 77,321,066 bp
  • T to A, chromosome 5 at 114,935,926 bp
  • A to T, chromosome 5 at 134,657,971 bp
  • T to A, chromosome 5 at 145,244,732 bp
  • T to A, chromosome 6 at 58,265,865 bp
  • A to G, chromosome 6 at 69,814,734 bp
  • A to G, chromosome 7 at 5,018,201 bp
  • G to A, chromosome 7 at 27,409,014 bp
  • T to A, chromosome 7 at 29,145,980 bp
  • T to C, chromosome 7 at 30,301,952 bp
  • A to G, chromosome 7 at 44,996,553 bp
  • T to C, chromosome 7 at 65,314,690 bp
  • G to A, chromosome 7 at 75,730,465 bp
  • A to G, chromosome 7 at 102,900,553 bp
  • T to G, chromosome 7 at 105,314,505 bp
  • A to G, chromosome 7 at 135,163,343 bp
  • A to T, chromosome 9 at 39,820,127 bp
  • T to A, chromosome 9 at 56,620,883 bp
  • C to T, chromosome 9 at 56,852,499 bp
  • T to C, chromosome 9 at 75,682,092 bp
  • G to A, chromosome 9 at 120,019,110 bp
  • A to G, chromosome 10 at 4,520,803 bp
  • T to G, chromosome 10 at 45,700,979 bp
  • A to T, chromosome 10 at 116,861,512 bp
  • T to C, chromosome 11 at 4,167,724 bp
  • T to A, chromosome 11 at 84,277,782 bp
  • T to A, chromosome 11 at 94,213,882 bp
  • T to C, chromosome 11 at 105,973,482 bp
  • C to T, chromosome 11 at 108,407,305 bp
  • G to A, chromosome 12 at 4,708,198 bp
  • A to T, chromosome 12 at 101,706,457 bp
  • A to G, chromosome 12 at 104,694,710 bp
  • A to T, chromosome 13 at 94,832,415 bp
  • T to C, chromosome 14 at 52,204,112 bp
  • G to A, chromosome 15 at 12,889,656 bp
  • A to G, chromosome 15 at 81,780,481 bp
  • A to G, chromosome 15 at 99,401,443 bp
  • A to T, chromosome 16 at 17,722,865 bp
  • T to C, chromosome 16 at 87,397,899 bp
  • A to G, chromosome 17 at 21,262,396 bp
  • T to C, chromosome 17 at 24,895,457 bp
  • G to A, chromosome 17 at 51,783,001 bp
  • G to T, chromosome 18 at 20,580,618 bp
  • A to T, chromosome 19 at 29,612,654 bp
  • A to C, chromosome 19 at 29,729,739 bp
  • A to G, chromosome 19 at 38,957,057 bp
  • A to G, chromosome 19 at 50,262,263 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7471 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045545-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.