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Strain Detail Sheet

Strain Name:
C57BL/6J-MtgxR7477Btlr/Mmmh
Stock Number:
045551-MU
Citation ID:
RRID:MMRRC_045551-MU
Other Names:
R7477 (G1)
Major Collection:
Beutler Mutagenetix

Strain Information

Gene Details

Aldh1l1
Name: aldehyde dehydrogenase 1 family, member L1
Synonyms: 1810048F20Rik, Fthfd
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 107747
HGNC: HGNC:3978
Homologene: 122031

Additional Resources
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Gene Ontology
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ClinGen
Grin3a
Name: glutamate receptor ionotropic, NMDA3A
Synonyms: NMDAR-L, NR3A, A830097C19Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242443
Homologene: 128613

Additional Resources
IMPC
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IMSR
Gene Ontology
BioGPS
Gene Cloud
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Mouse Phenome DB
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KOMP Phenotyping
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Mapk14
Name: mitogen-activated protein kinase 14
Synonyms: p38-alpha, p38, p38 MAP Kinase, Mxi2, CSBP2, Crk1, Csbp1, p38MAPK, p38a, p38 alpha, p38alpha, p38 MAP kinase alpha, p38 MAPK
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 26416
HGNC: HGNC:6876
Homologene: 31777

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ClinGen
Trp53bp1
Name: transformation related protein 53 binding protein 1
Synonyms: 53BP1, p53BP1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 27223
Homologene: 4137

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
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Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Adipor2
Name: adiponectin receptor 2
Synonyms: 1110001I14Rik, D6Ucla1e
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 68465
Homologene: 56119

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Washc4
Name: WASH complex subunit 4
Synonyms: A230046K03Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 319277
VEGA: 10
Homologene: 123926

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Ell
Name: elongation factor RNA polymerase II
Synonyms: Men, Ell1, eleven-nineteen lysine-rich leukemia gene
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 13716
Homologene: 4762

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 60,261,584 bp
  • A to G, chromosome 1 at 93,581,028 bp
  • C to T, chromosome 1 at 173,221,284 bp
  • T to C, chromosome 2 at 3,225,639 bp
  • T to A, chromosome 2 at 24,279,692 bp
  • C to A, chromosome 2 at 30,107,976 bp
  • A to G, chromosome 2 at 87,532,087 bp
  • A to C, chromosome 2 at 121,236,346 bp
  • G to A, chromosome 2 at 152,920,341 bp
  • G to A, chromosome 3 at 14,776,283 bp
  • C to A, chromosome 3 at 64,398,429 bp
  • C to T, chromosome 3 at 64,491,639 bp
  • T to C, chromosome 3 at 97,658,747 bp
  • A to T, chromosome 4 at 46,157,897 bp
  • G to A, chromosome 4 at 49,719,278 bp
  • G to A, chromosome 4 at 86,415,651 bp
  • G to T, chromosome 5 at 35,592,103 bp
  • A to G, chromosome 5 at 37,173,571 bp
  • T to C, chromosome 5 at 87,336,620 bp
  • A to T, chromosome 5 at 92,650,696 bp
  • G to A, chromosome 5 at 104,483,242 bp
  • A to C, chromosome 5 at 108,220,850 bp
  • A to T, chromosome 5 at 109,059,348 bp
  • A to T, chromosome 5 at 139,547,262 bp
  • T to C, chromosome 6 at 90,598,387 bp
  • A to T, chromosome 6 at 119,361,922 bp
  • A to G, chromosome 7 at 24,714,248 bp
  • G to A, chromosome 7 at 45,728,980 bp
  • C to T, chromosome 7 at 75,749,247 bp
  • A to C, chromosome 7 at 96,845,808 bp
  • A to T, chromosome 7 at 126,444,818 bp
  • C to A, chromosome 7 at 141,816,282 bp
  • A to G, chromosome 7 at 142,537,281 bp
  • T to A, chromosome 8 at 33,615,474 bp
  • T to A, chromosome 8 at 43,569,070 bp
  • T to C, chromosome 8 at 70,585,218 bp
  • A to T, chromosome 8 at 122,894,385 bp
  • A to G, chromosome 9 at 110,087,140 bp
  • G to A, chromosome 9 at 111,364,957 bp
  • T to C, chromosome 10 at 63,125,721 bp
  • T to C, chromosome 10 at 78,714,304 bp
  • T to C, chromosome 10 at 83,574,443 bp
  • T to A, chromosome 10 at 127,568,920 bp
  • T to A, chromosome 10 at 128,435,434 bp
  • A to G, chromosome 11 at 65,992,731 bp
  • G to T, chromosome 11 at 97,335,839 bp
  • A to G, chromosome 12 at 11,271,807 bp
  • A to G, chromosome 12 at 24,653,563 bp
  • T to A, chromosome 12 at 72,503,527 bp
  • A to T, chromosome 12 at 76,628,565 bp
  • A to T, chromosome 12 at 76,953,186 bp
  • T to A, chromosome 12 at 83,979,830 bp
  • A to G, chromosome 13 at 20,285,319 bp
  • A to T, chromosome 13 at 38,172,863 bp
  • A to T, chromosome 13 at 41,318,480 bp
  • T to A, chromosome 14 at 19,805,973 bp
  • T to C, chromosome 14 at 63,606,680 bp
  • A to G, chromosome 15 at 23,410,725 bp
  • T to C, chromosome 15 at 76,455,326 bp
  • C to T, chromosome 15 at 91,812,325 bp
  • A to G, chromosome 16 at 3,964,811 bp
  • C to A, chromosome 16 at 32,677,891 bp
  • G to A, chromosome 17 at 28,745,078 bp
  • G to A, chromosome 17 at 54,281,759 bp
  • A to C, chromosome 17 at 64,309,645 bp
  • T to C, chromosome 18 at 80,440,714 bp
  • A to G, chromosome 19 at 6,062,471 bp
  • T to A, chromosome 19 at 41,916,816 bp

Strain Description

Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.

Strain Origin

Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7477 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
Black

Breeding

MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain

Reproductive Statistics

Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
045551-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.