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Strain Detail Sheet

Strain Name:
C57BL/6J-MtgxR7514Btlr/Mmmh
Stock Number:
045587-MU
Citation ID:
RRID:MMRRC_045587-MU
Other Names:
R7514 (G1)
Major Collection:
Beutler Mutagenetix

Strain Information

Gene Details

Twist1
Name: twist basic helix-loop-helix transcription factor 1
Synonyms: M-Twist, Pluridigite, pdt, charlie chaplin, Skam10Jus, Ska10, bHLHa38
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 22160
VEGA: 12
Homologene: 402

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ClinGen
Utrn
Name: utrophin
Synonyms: G-utrophin, Dmdl, DRP
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 22288
VEGA: 10
Homologene: 21398

Additional Resources
IMPC
Google
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Gene Ontology
BioGPS
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Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
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ClinGen
Lrig2
Name: leucine-rich repeats and immunoglobulin-like domains 2
Synonyms: 4632419I10Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 269473
Homologene: 8882

Additional Resources
IMPC
Google
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IMSR
Gene Ontology
BioGPS
Gene Cloud
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Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
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ClinGen
Ank2
Name: ankyrin 2, brain
Synonyms: ankyrin B, Ank-2, Ankyrin-2, Ankyrin-B, Gm4392
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 109676
HGNC: HGNC:493

Additional Resources
IMPC
Google
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Gene Ontology
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ClinGen
Vcan
Name: versican
Synonyms: PG-M, hdf, heart defect, 5430420N07Rik, Cspg2, DPEAAE
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 13003
HGNC: HGNC:2464
Homologene: 3228

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Sox10
Name: SRY (sex determining region Y)-box 10
Synonyms: Sox21, gt
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 20665
VEGA: 15
Homologene: 5055

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Tjp2
Name: tight junction protein 2
Synonyms: ZO-2
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 21873
VEGA: 19
Homologene: 3541

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 20,805,896 bp
  • T to C, chromosome 1 at 43,569,221 bp
  • A to G, chromosome 1 at 74,528,762 bp
  • C to T, chromosome 1 at 85,681,139 bp
  • A to G, chromosome 1 at 93,816,442 bp
  • A to T, chromosome 1 at 106,780,502 bp
  • G to A, chromosome 1 at 106,950,804 bp
  • A to G, chromosome 1 at 134,410,530 bp
  • G to A, chromosome 1 at 149,851,362 bp
  • A to G, chromosome 1 at 156,524,754 bp
  • T to G, chromosome 1 at 171,059,343 bp
  • T to C, chromosome 1 at 175,906,666 bp
  • T to C, chromosome 1 at 181,628,067 bp
  • C to T, chromosome 2 at 30,404,565 bp
  • A to G, chromosome 2 at 32,387,849 bp
  • A to G, chromosome 2 at 36,812,639 bp
  • G to T, chromosome 2 at 37,537,342 bp
  • A to T, chromosome 2 at 86,131,628 bp
  • A to G, chromosome 2 at 158,217,353 bp
  • T to A, chromosome 2 at 179,890,843 bp
  • G to A, chromosome 3 at 5,242,207 bp
  • G to C, chromosome 3 at 90,210,459 bp
  • C to T, chromosome 3 at 95,007,530 bp
  • T to A, chromosome 3 at 103,163,062 bp
  • A to G, chromosome 3 at 104,465,760 bp
  • G to A, chromosome 3 at 127,025,603 bp
  • A to T, chromosome 4 at 104,846,050 bp
  • T to A, chromosome 4 at 120,096,855 bp
  • T to C, chromosome 4 at 139,452,655 bp
  • A to T, chromosome 4 at 144,703,228 bp
  • A to G, chromosome 5 at 13,523,126 bp
  • T to C, chromosome 5 at 67,348,078 bp
  • T to G, chromosome 5 at 86,497,317 bp
  • T to C, chromosome 5 at 110,432,933 bp
  • C to T, chromosome 5 at 114,670,460 bp
  • A to G, chromosome 5 at 116,446,511 bp
  • A to G, chromosome 6 at 41,373,914 bp
  • T to C, chromosome 6 at 136,854,741 bp
  • T to C, chromosome 7 at 45,501,718 bp
  • A to G, chromosome 7 at 66,172,120 bp
  • T to C, chromosome 7 at 144,517,713 bp
  • T to C, chromosome 8 at 14,975,956 bp
  • T to C, chromosome 8 at 22,775,642 bp
  • A to C, chromosome 8 at 25,792,209 bp
  • A to T, chromosome 8 at 80,717,534 bp
  • T to G, chromosome 8 at 122,635,126 bp
  • A to T, chromosome 9 at 19,137,865 bp
  • G to T, chromosome 9 at 21,342,472 bp
  • A to T, chromosome 9 at 22,360,857 bp
  • T to A, chromosome 9 at 38,582,051 bp
  • G to A, chromosome 9 at 38,775,678 bp
  • T to C, chromosome 9 at 65,078,754 bp
  • A to G, chromosome 9 at 65,706,136 bp
  • T to C, chromosome 9 at 71,284,963 bp
  • A to G, chromosome 9 at 99,620,545 bp
  • A to G, chromosome 9 at 111,364,756 bp
  • T to A, chromosome 10 at 4,546,839 bp
  • A to G, chromosome 10 at 12,698,089 bp
  • A to T, chromosome 10 at 49,523,808 bp
  • G to T, chromosome 10 at 75,776,791 bp
  • T to C, chromosome 10 at 84,880,226 bp
  • G to T, chromosome 10 at 89,592,107 bp
  • A to C, chromosome 11 at 60,801,986 bp
  • G to T, chromosome 11 at 62,265,320 bp
  • G to A, chromosome 11 at 67,243,322 bp
  • G to A, chromosome 11 at 69,060,584 bp
  • C to T, chromosome 11 at 70,218,884 bp
  • A to G, chromosome 11 at 72,195,802 bp
  • C to T, chromosome 11 at 95,065,896 bp
  • T to C, chromosome 11 at 98,222,658 bp
  • A to G, chromosome 11 at 106,409,893 bp
  • T to C, chromosome 12 at 33,958,356 bp
  • C to A, chromosome 12 at 76,328,529 bp
  • T to C, chromosome 12 at 78,626,165 bp
  • T to G, chromosome 12 at 84,357,738 bp
  • C to T, chromosome 12 at 85,030,494 bp
  • C to T, chromosome 12 at 102,369,650 bp
  • C to T, chromosome 12 at 111,823,815 bp
  • T to C, chromosome 12 at 112,929,052 bp
  • G to A, chromosome 13 at 33,897,403 bp
  • T to C, chromosome 13 at 89,704,118 bp
  • A to T, chromosome 13 at 102,787,426 bp
  • A to T, chromosome 14 at 4,399,690 bp
  • G to T, chromosome 14 at 12,179,342 bp
  • A to C, chromosome 14 at 78,982,234 bp
  • G to A, chromosome 15 at 37,988,237 bp
  • G to A, chromosome 15 at 79,156,221 bp
  • C to A, chromosome 15 at 89,120,525 bp
  • T to C, chromosome 15 at 98,774,164 bp
  • G to A, chromosome 15 at 101,553,170 bp
  • A to T, chromosome 16 at 31,154,567 bp
  • A to G, chromosome 16 at 56,705,609 bp
  • T to G, chromosome 16 at 91,654,860 bp
  • A to G, chromosome 17 at 5,341,714 bp
  • T to C, chromosome 17 at 18,171,271 bp
  • A to G, chromosome 17 at 20,029,529 bp
  • A to G, chromosome 17 at 21,463,500 bp
  • T to A, chromosome 17 at 22,548,399 bp
  • T to C, chromosome 17 at 23,308,061 bp
  • T to C, chromosome 17 at 34,196,665 bp
  • T to C, chromosome 17 at 57,107,238 bp
  • T to A, chromosome 18 at 57,363,253 bp
  • C to T, chromosome 18 at 58,612,221 bp
  • C to T, chromosome 19 at 24,111,522 bp

Strain Description

Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.

Strain Origin

Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7514 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
Black

Breeding

MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain

Reproductive Statistics

Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
045587-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.