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Strain Name:
C57BL/6J-MtgxR7548Btlr/Mmmh
Stock Number:
045619-MU
Citation ID:
RRID:MMRRC_045619-MU
Other Names:
R7548 (G1)
Major Collection:

Strain Information

Hlcs
Name: holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase)
Synonyms: 410I21.SP6, D16Jhu34
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 110948
VEGA: 16
HGNC: HGNC:4976
Homologene: 37302
Dlc1
Name: deleted in liver cancer 1
Synonyms: p122-RhoGAP, STARD12, Arhgap7, A730069N07Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 50768
HGNC: HGNC:2897
Homologene: 4442
Emb
Name: embigin
Synonyms: Gp70
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 13723
Homologene: 7743
Slc1a6
Name: solute carrier family 1 (high affinity aspartate/glutamate transporter), member 6
Synonyms: EAAT4
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 20513
VEGA: 10
Homologene: 21055
Cnot7
Name: CCR4-NOT transcription complex, subunit 7
Synonyms: Caf1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 18983
Homologene: 49011
Dnmbp
Name: dynamin binding protein
Synonyms: 2410003M15Rik, Tuba, 2410003L07Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 71972
Homologene: 9061
Fryl
Name: FRY like transcription coactivator
Synonyms: 2310004H21Rik, 2510002A14Rik, 9030227G01Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 72313
Homologene: 103956
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 52,663,287 bp
  • G to A, chromosome 1 at 87,844,916 bp
  • A to T, chromosome 1 at 133,701,641 bp
  • A to T, chromosome 2 at 29,795,408 bp
  • T to C, chromosome 2 at 89,249,086 bp
  • T to A, chromosome 2 at 126,389,342 bp
  • A to G, chromosome 2 at 155,909,389 bp
  • A to T, chromosome 3 at 38,981,114 bp
  • G to A, chromosome 3 at 97,205,893 bp
  • A to C, chromosome 3 at 114,123,760 bp
  • C to T, chromosome 4 at 41,423,601 bp
  • T to C, chromosome 4 at 44,994,724 bp
  • T to C, chromosome 4 at 129,929,809 bp
  • T to A, chromosome 4 at 154,856,359 bp
  • T to A, chromosome 5 at 12,577,816 bp
  • T to A, chromosome 5 at 67,815,728 bp
  • T to C, chromosome 5 at 72,760,350 bp
  • T to A, chromosome 5 at 73,191,762 bp
  • G to A, chromosome 5 at 114,877,998 bp
  • A to T, chromosome 5 at 138,251,231 bp
  • T to C, chromosome 5 at 145,986,925 bp
  • C to T, chromosome 6 at 72,345,013 bp
  • G to A, chromosome 6 at 73,027,440 bp
  • C to G, chromosome 6 at 113,466,387 bp
  • T to A, chromosome 6 at 120,761,714 bp
  • A to T, chromosome 6 at 122,526,034 bp
  • A to G, chromosome 6 at 134,507,508 bp
  • T to A, chromosome 6 at 147,257,155 bp
  • T to A, chromosome 7 at 13,301,418 bp
  • A to T, chromosome 7 at 25,530,533 bp
  • G to A, chromosome 7 at 26,450,179 bp
  • A to G, chromosome 7 at 29,709,826 bp
  • A to T, chromosome 7 at 118,126,510 bp
  • A to G, chromosome 8 at 36,584,655 bp
  • A to G, chromosome 8 at 40,500,833 bp
  • C to A, chromosome 8 at 72,207,197 bp
  • A to G, chromosome 8 at 104,931,906 bp
  • A to T, chromosome 9 at 9,673,410 bp
  • T to C, chromosome 9 at 37,277,427 bp
  • T to C, chromosome 9 at 65,037,235 bp
  • A to T, chromosome 10 at 62,666,167 bp
  • G to T, chromosome 10 at 78,814,431 bp
  • G to T, chromosome 10 at 84,947,872 bp
  • C to T, chromosome 11 at 5,902,040 bp
  • A to G, chromosome 11 at 51,789,267 bp
  • A to T, chromosome 11 at 62,713,875 bp
  • G to A, chromosome 11 at 73,588,976 bp
  • T to C, chromosome 11 at 100,049,520 bp
  • T to C, chromosome 11 at 114,987,422 bp
  • T to C, chromosome 11 at 118,396,843 bp
  • A to T, chromosome 13 at 53,367,932 bp
  • A to G, chromosome 13 at 59,716,654 bp
  • C to A, chromosome 13 at 84,222,418 bp
  • T to A, chromosome 13 at 117,272,054 bp
  • T to C, chromosome 14 at 44,410,688 bp
  • T to C, chromosome 14 at 45,334,918 bp
  • T to C, chromosome 14 at 75,042,352 bp
  • C to T, chromosome 15 at 6,429,918 bp
  • T to C, chromosome 15 at 82,244,679 bp
  • C to A, chromosome 15 at 83,117,670 bp
  • C to A, chromosome 15 at 84,757,058 bp
  • T to C, chromosome 15 at 89,388,704 bp
  • T to C, chromosome 15 at 89,574,393 bp
  • T to C, chromosome 15 at 101,053,198 bp
  • A to G, chromosome 16 at 38,754,066 bp
  • T to A, chromosome 16 at 94,133,017 bp
  • T to C, chromosome 17 at 24,896,548 bp
  • T to C, chromosome 17 at 69,210,276 bp
  • C to G, chromosome 17 at 71,789,968 bp
  • C to A, chromosome 17 at 83,425,337 bp
  • C to T, chromosome 18 at 76,860,905 bp
  • T to C, chromosome 19 at 43,889,399 bp
  • T to C, chromosome 19 at 44,110,550 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7548 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045619-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.