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Strain Name:
C57BL/6J-MtgxR7560Btlr/Mmmh
Stock Number:
045626-MU
Citation ID:
RRID:MMRRC_045626-MU
Other Names:
R7560 (G1)
Major Collection:

Strain Information

Pkd1
Name: polycystin 1, transient receptor potential channel interacting
Synonyms: polycystin-1, PC1, PC-1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 18763
VEGA: 17
HGNC: HGNC:9008
Homologene: 250
Nfat5
Name: nuclear factor of activated T cells 5
Synonyms: nfatz, TonEBP, B130038B15Rik, OREBP
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 54446
HGNC: HGNC:7774
Homologene: 4811
Bmp7
Name: bone morphogenetic protein 7
Synonyms: osteogenic protein 1, OP1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 12162
HGNC: HGNC:1074
Homologene: 20410
Cdkn2d
Name: cyclin dependent kinase inhibitor 2D
Synonyms: p19, INK4d, p19INK4d, INK4d
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 12581
VEGA: 9
HGNC: HGNC:1790
Homologene: 36081
D630045J12Rik
Name: RIKEN cDNA D630045J12 gene
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 330286
Homologene: 19782
Mycn
Name: v-myc avian myelocytomatosis viral related oncogene, neuroblastoma derived
Synonyms: Nmyc, N-myc, Nmyc-1, Nmyc1, bHLHe37
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 18109
HGNC: HGNC:7559
Homologene: 3922
Lrrn3
Name: leucine rich repeat protein 3, neuronal
Synonyms: NLRR-3
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 16981
VEGA: 12
Homologene: 36315
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 34,182,451 bp
  • T to A, chromosome 1 at 60,010,210 bp
  • A to G, chromosome 1 at 162,888,749 bp
  • G to A, chromosome 2 at 26,460,165 bp
  • A to G, chromosome 2 at 28,547,595 bp
  • A to G, chromosome 2 at 31,457,173 bp
  • G to A, chromosome 2 at 66,217,204 bp
  • T to C, chromosome 2 at 86,978,778 bp
  • T to C, chromosome 2 at 91,942,815 bp
  • T to A, chromosome 2 at 105,794,588 bp
  • A to G, chromosome 2 at 118,715,643 bp
  • A to G, chromosome 2 at 164,895,992 bp
  • C to T, chromosome 2 at 168,925,665 bp
  • C to T, chromosome 2 at 172,939,964 bp
  • C to A, chromosome 2 at 173,118,406 bp
  • T to C, chromosome 2 at 179,890,902 bp
  • G to A, chromosome 2 at 181,587,082 bp
  • T to G, chromosome 3 at 20,030,452 bp
  • T to C, chromosome 3 at 79,623,579 bp
  • T to C, chromosome 3 at 90,224,899 bp
  • G to T, chromosome 3 at 92,685,841 bp
  • T to C, chromosome 4 at 59,076,140 bp
  • G to A, chromosome 4 at 116,051,494 bp
  • A to G, chromosome 4 at 126,236,636 bp
  • T to G, chromosome 5 at 65,791,115 bp
  • A to T, chromosome 5 at 76,242,891 bp
  • T to A, chromosome 5 at 104,480,353 bp
  • C to T, chromosome 5 at 110,127,291 bp
  • A to G, chromosome 5 at 121,254,342 bp
  • G to T, chromosome 6 at 29,886,140 bp
  • T to A, chromosome 6 at 34,163,361 bp
  • T to A, chromosome 6 at 38,196,627 bp
  • A to T, chromosome 7 at 12,193,823 bp
  • T to C, chromosome 7 at 13,131,818 bp
  • T to A, chromosome 7 at 25,058,526 bp
  • G to A, chromosome 7 at 25,272,853 bp
  • G to A, chromosome 7 at 25,681,359 bp
  • T to C, chromosome 7 at 27,749,136 bp
  • T to A, chromosome 7 at 27,749,271 bp
  • T to C, chromosome 7 at 30,567,351 bp
  • T to A, chromosome 7 at 66,171,244 bp
  • T to C, chromosome 7 at 66,171,571 bp
  • TCCCAGGGCC to TCC, chromosome 7 at 80,098,331 bp
  • A to T, chromosome 7 at 85,623,907 bp
  • T to A, chromosome 7 at 89,407,553 bp
  • T to G, chromosome 7 at 98,710,231 bp
  • T to C, chromosome 7 at 102,781,682 bp
  • A to G, chromosome 7 at 126,459,656 bp
  • C to A, chromosome 7 at 126,703,134 bp
  • A to T, chromosome 7 at 141,231,225 bp
  • A to G, chromosome 7 at 142,000,860 bp
  • A to G, chromosome 7 at 143,080,986 bp
  • T to C, chromosome 8 at 14,822,697 bp
  • A to G, chromosome 8 at 27,494,960 bp
  • A to G, chromosome 8 at 107,169,859 bp
  • T to A, chromosome 8 at 107,370,589 bp
  • T to C, chromosome 9 at 9,035,531 bp
  • A to T, chromosome 9 at 15,996,842 bp
  • G to T, chromosome 9 at 21,289,244 bp
  • A to T, chromosome 9 at 45,856,139 bp
  • A to G, chromosome 9 at 53,375,773 bp
  • T to C, chromosome 9 at 74,210,126 bp
  • G to A, chromosome 10 at 13,168,785 bp
  • T to C, chromosome 10 at 82,284,615 bp
  • T to C, chromosome 10 at 89,498,261 bp
  • C to A, chromosome 11 at 46,459,062 bp
  • T to A, chromosome 11 at 62,128,409 bp
  • T to A, chromosome 11 at 65,775,757 bp
  • A to G, chromosome 11 at 69,356,270 bp
  • G to T, chromosome 11 at 99,000,837 bp
  • C to T, chromosome 11 at 113,663,389 bp
  • A to C, chromosome 12 at 12,940,291 bp
  • T to A, chromosome 12 at 41,452,713 bp
  • A to T, chromosome 12 at 57,306,073 bp
  • T to C, chromosome 12 at 65,011,142 bp
  • G to T, chromosome 12 at 111,807,902 bp
  • T to G, chromosome 12 at 112,779,674 bp
  • A to G, chromosome 13 at 53,110,813 bp
  • A to G, chromosome 13 at 91,784,391 bp
  • A to G, chromosome 14 at 22,828,702 bp
  • T to C, chromosome 14 at 23,530,242 bp
  • T to C, chromosome 14 at 29,058,421 bp
  • T to C, chromosome 14 at 31,304,983 bp
  • T to C, chromosome 14 at 52,463,394 bp
  • T to C, chromosome 15 at 8,155,047 bp
  • G to A, chromosome 15 at 59,366,192 bp
  • G to T, chromosome 15 at 76,538,469 bp
  • T to A, chromosome 15 at 101,690,559 bp
  • A to G, chromosome 16 at 4,992,191 bp
  • A to C, chromosome 16 at 15,862,805 bp
  • G to A, chromosome 16 at 90,940,483 bp
  • T to C, chromosome 17 at 23,824,976 bp
  • G to A, chromosome 17 at 24,573,631 bp
  • A to G, chromosome 17 at 25,092,341 bp
  • G to A, chromosome 17 at 44,085,264 bp
  • T to C, chromosome 17 at 53,679,267 bp
  • C to A, chromosome 18 at 61,312,210 bp
  • A to T, chromosome 19 at 5,384,877 bp
  • A to G, chromosome 19 at 17,836,972 bp
  • C to G, chromosome 19 at 39,807,214 bp
  • T to A, chromosome 19 at 40,952,390 bp
  • T to C, chromosome 19 at 56,722,688 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7560 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045626-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.