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Strain Name:
C57BL/6J-MtgxR7666Btlr/Mmmh
Stock Number:
045705-MU
Citation ID:
RRID:MMRRC_045705-MU
Other Names:
R7666 (G1)
Major Collection:

Strain Information

Ltbp3
Name: latent transforming growth factor beta binding protein 3
Synonyms: Ltbp2
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 16998
VEGA: 19
HGNC: HGNC:6716
Homologene: 7405
Atl3
Name: atlastin GTPase 3
Synonyms: 4633402C03Rik, 5730596K20Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 109168
VEGA: 19
Homologene: 9149
Arid5b
Name: AT-rich interaction domain 5B
Synonyms: 5430435G07Rik, Desrt, Mrf2beta, Mrf2alpha, Mrf2
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 71371
VEGA: 10
Homologene: 45872
Crybg3
Name: beta-gamma crystallin domain containing 3
Synonyms: Gm9581
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 224273
Homologene: 28544
Ppp1r9a
Name: protein phosphatase 1, regulatory subunit 9A
Synonyms: 4930518N04Rik, neurabin-I, A230094E16Rik, 2810430P21Rik, Neurabin I, NRB
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 243725
Homologene: 14247
Cilk1
Name: ciliogenesis associated kinase 1
Synonyms: 2210420N10Rik, Ick
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 56542
Homologene: 69218
Gk5
Name: glycerol kinase 5
Synonyms: G630067D24Rik, C330018K18Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235533
Homologene: 15141
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 37,431,415 bp
  • A to T, chromosome 1 at 53,547,297 bp
  • A to T, chromosome 1 at 87,209,453 bp
  • A to G, chromosome 1 at 195,154,225 bp
  • C to T, chromosome 2 at 31,380,233 bp
  • A to G, chromosome 2 at 58,934,500 bp
  • C to T, chromosome 2 at 82,259,060 bp
  • C to T, chromosome 2 at 125,306,471 bp
  • A to G, chromosome 3 at 14,870,064 bp
  • A to G, chromosome 3 at 108,398,588 bp
  • A to G, chromosome 4 at 22,376,869 bp
  • T to C, chromosome 4 at 59,885,047 bp
  • T to A, chromosome 4 at 63,132,717 bp
  • C to A, chromosome 4 at 117,213,741 bp
  • T to A, chromosome 4 at 118,934,264 bp
  • A to G, chromosome 4 at 139,413,480 bp
  • G to A, chromosome 4 at 139,633,957 bp
  • A to T, chromosome 4 at 143,728,515 bp
  • A to T, chromosome 6 at 5,143,238 bp
  • C to T, chromosome 6 at 12,379,438 bp
  • A to C, chromosome 6 at 48,659,155 bp
  • A to T, chromosome 6 at 51,466,937 bp
  • C to A, chromosome 6 at 121,861,220 bp
  • G to A, chromosome 6 at 136,697,968 bp
  • A to G, chromosome 7 at 100,565,201 bp
  • T to C, chromosome 7 at 121,649,181 bp
  • C to T, chromosome 7 at 130,716,814 bp
  • A to G, chromosome 8 at 3,541,591 bp
  • T to C, chromosome 8 at 22,090,517 bp
  • T to C, chromosome 9 at 18,558,427 bp
  • A to G, chromosome 9 at 38,733,963 bp
  • G to C, chromosome 9 at 78,167,620 bp
  • T to A, chromosome 9 at 96,153,107 bp
  • A to T, chromosome 9 at 124,440,873 bp
  • C to T, chromosome 10 at 68,098,587 bp
  • A to G, chromosome 11 at 5,897,140 bp
  • A to T, chromosome 11 at 67,256,281 bp
  • T to A, chromosome 12 at 58,267,757 bp
  • C to A, chromosome 12 at 116,841,320 bp
  • G to A, chromosome 13 at 17,772,576 bp
  • A to G, chromosome 13 at 34,128,135 bp
  • C to T, chromosome 13 at 62,171,411 bp
  • A to C, chromosome 13 at 65,036,000 bp
  • A to T, chromosome 14 at 14,176,075 bp
  • G to A, chromosome 14 at 24,157,799 bp
  • T to C, chromosome 14 at 28,518,372 bp
  • C to T, chromosome 14 at 31,240,354 bp
  • T to A, chromosome 15 at 4,789,505 bp
  • A to T, chromosome 16 at 59,559,337 bp
  • T to C, chromosome 17 at 28,810,572 bp
  • C to T, chromosome 17 at 35,526,486 bp
  • C to T, chromosome 19 at 5,747,006 bp
  • T to C, chromosome 19 at 7,510,040 bp
  • A to G, chromosome 19 at 12,920,162 bp
  • A to T, chromosome 19 at 23,229,017 bp
  • A to T, chromosome 19 at 39,438,242 bp
  • C to T, chromosome X at 8,962,804 bp
  • C to T, chromosome X at 8,962,808 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7666 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045705-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.