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Strain Name:
C57BL/6J-MtgxR7685Btlr/Mmmh
Stock Number:
045750-MU
Citation ID:
RRID:MMRRC_045750-MU
Other Names:
R7685 (G1)
Major Collection:

Strain Information

Ubn2
Name: ubinuclein 2
Synonyms: 6030408G03Rik, 2900060J04Rik, D130059P03Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 320538
Homologene: 45564
Ptprz1
Name: protein tyrosine phosphatase receptor type Z, polypeptide 1
Synonyms: DSD-1-PG, phosphacan, PTPzeta, PTPbeta, Rptpbeta, Ptpz, Ptprz, RPTPz
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 19283
HGNC: HGNC:9685
Homologene: 2136
Lyst
Name: lysosomal trafficking regulator
Synonyms: D13Sfk13
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 17101
VEGA: 13
HGNC: HGNC:1968
Homologene: 61
Glrx3
Name: glutaredoxin 3
Synonyms: PKC interacting cousin of thioredoxin, PICOT, Txnl2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 30926
Homologene: 4769
Poli
Name: polymerase (DNA directed), iota
Synonyms: Rad30b
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 26447
HGNC: HGNC:9182
Homologene: 5209
Mlst8
Name: MTOR associated protein, LST8 homolog (S. cerevisiae)
Synonyms: 0610033N12Rik, mLST8, Gbl
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 56716
VEGA: 17
Homologene: 6833
Mllt6
Name: myeloid/lymphoid or mixed-lineage leukemia; translocated to, 6
Synonyms: Af17
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 246198
HGNC: HGNC:7138
Homologene: 31347
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 153,458,406 bp
  • T to C, chromosome 1 at 162,958,332 bp
  • G to A, chromosome 2 at 5,054,650 bp
  • C to T, chromosome 2 at 30,404,565 bp
  • C to T, chromosome 2 at 34,779,296 bp
  • C to T, chromosome 2 at 66,676,192 bp
  • C to A, chromosome 2 at 92,383,472 bp
  • T to A, chromosome 2 at 120,433,792 bp
  • C to A, chromosome 2 at 122,068,880 bp
  • T to A, chromosome 3 at 19,227,745 bp
  • T to A, chromosome 3 at 27,896,313 bp
  • T to A, chromosome 3 at 51,469,974 bp
  • T to A, chromosome 4 at 134,884,509 bp
  • T to A, chromosome 4 at 143,897,801 bp
  • T to A, chromosome 4 at 147,582,874 bp
  • T to C, chromosome 4 at 154,871,654 bp
  • T to A, chromosome 5 at 14,680,616 bp
  • A to C, chromosome 5 at 31,168,267 bp
  • C to A, chromosome 5 at 104,047,080 bp
  • A to G, chromosome 5 at 104,968,215 bp
  • C to T, chromosome 5 at 123,865,333 bp
  • A to T, chromosome 6 at 23,024,978 bp
  • C to A, chromosome 6 at 28,244,194 bp
  • A to G, chromosome 6 at 38,491,792 bp
  • A to T, chromosome 6 at 100,804,528 bp
  • A to T, chromosome 6 at 128,384,958 bp
  • T to G, chromosome 6 at 132,755,093 bp
  • T to C, chromosome 7 at 83,597,458 bp
  • G to T, chromosome 7 at 127,273,334 bp
  • T to C, chromosome 7 at 128,113,242 bp
  • T to C, chromosome 7 at 137,459,191 bp
  • T to C, chromosome 7 at 140,574,246 bp
  • C to T, chromosome 7 at 141,809,383 bp
  • C to T, chromosome 8 at 60,933,085 bp
  • A to T, chromosome 8 at 84,832,351 bp
  • A to G, chromosome 8 at 94,521,400 bp
  • A to G, chromosome 8 at 113,713,223 bp
  • G to A, chromosome 8 at 122,413,876 bp
  • C to T, chromosome 9 at 54,628,559 bp
  • A to T, chromosome 10 at 8,207,575 bp
  • T to C, chromosome 10 at 40,574,089 bp
  • T to C, chromosome 10 at 76,422,808 bp
  • A to G, chromosome 10 at 99,109,964 bp
  • A to C, chromosome 10 at 100,540,057 bp
  • T to A, chromosome 10 at 107,643,978 bp
  • T to A, chromosome 10 at 111,276,509 bp
  • T to C, chromosome 11 at 67,240,930 bp
  • T to C, chromosome 11 at 97,676,964 bp
  • T to C, chromosome 12 at 35,504,017 bp
  • A to G, chromosome 13 at 13,669,865 bp
  • T to A, chromosome 13 at 19,650,975 bp
  • A to G, chromosome 13 at 34,652,284 bp
  • G to A, chromosome 13 at 50,425,644 bp
  • G to T, chromosome 13 at 81,103,324 bp
  • T to C, chromosome 14 at 8,090,453 bp
  • G to T, chromosome 14 at 48,280,034 bp
  • A to T, chromosome 14 at 50,710,758 bp
  • G to A, chromosome 14 at 55,897,734 bp
  • A to G, chromosome 14 at 79,098,300 bp
  • A to G, chromosome 14 at 119,075,347 bp
  • C to T, chromosome 15 at 59,316,041 bp
  • C to A, chromosome 15 at 76,595,388 bp
  • G to T, chromosome 15 at 85,978,732 bp
  • T to C, chromosome 15 at 89,067,770 bp
  • T to C, chromosome 17 at 15,059,462 bp
  • T to C, chromosome 17 at 24,476,057 bp
  • C to T, chromosome 17 at 30,657,973 bp
  • C to T, chromosome 17 at 43,479,174 bp
  • G to T, chromosome 17 at 50,047,380 bp
  • T to A, chromosome 17 at 56,102,413 bp
  • T to A, chromosome 18 at 9,993,454 bp
  • T to C, chromosome 18 at 34,314,208 bp
  • T to C, chromosome 18 at 70,525,519 bp
  • A to G, chromosome 19 at 4,906,154 bp
  • A to G, chromosome 19 at 8,896,804 bp
  • A to G, chromosome 19 at 24,727,851 bp
  • A to T, chromosome 19 at 34,612,555 bp
  • G to A, chromosome 19 at 38,748,433 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7685 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045750-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.