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Strain Name:
C57BL/6J-MtgxR7747Btlr/Mmmh
Stock Number:
045803-MU
Citation ID:
RRID:MMRRC_045803-MU
Other Names:
R7747 (G1)
Major Collection:

Strain Information

Prkar2b
Name: protein kinase, cAMP dependent regulatory, type II beta
Synonyms: Pkarb2, RII(beta), PKARIIbeta
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 19088
HGNC: HGNC:9392
Homologene: 37666
Adamts20
Name: ADAM metallopeptidase with thrombospondin type 1 motif 20
Synonyms: ADAMTS-20, bt
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 223838
Homologene: 11808
Sp4
Name: trans-acting transcription factor 4
Synonyms: HF1-b, HF-1b, 5730497N03Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 20688
VEGA: 12
Homologene: 2341
Pofut2
Name: protein O-fucosyltransferase 2
Synonyms: FUT13, 2310011G23Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 80294
VEGA: 10
Homologene: 12724
Vps41
Name: VPS41 HOPS complex subunit
Synonyms: Vam2, mVam2
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 218035
VEGA: 13
Homologene: 69165
Bcl2l2
Name: BCL2-like 2
Synonyms: bclw, Gtrgal2, Gtrosa41, Bcl-w
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 12050
HGNC: HGNC:995
Homologene: 2989
Tert
Name: telomerase reverse transcriptase
Synonyms: TR
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 21752
VEGA: 13
Homologene: 31141
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 12,990,122 bp
  • T to C, chromosome 1 at 52,676,547 bp
  • A to G, chromosome 1 at 130,743,547 bp
  • A to G, chromosome 1 at 157,565,472 bp
  • T to A, chromosome 1 at 186,968,677 bp
  • G to A, chromosome 2 at 16,074,835 bp
  • A to G, chromosome 2 at 29,871,286 bp
  • G to A, chromosome 2 at 30,090,584 bp
  • C to T, chromosome 2 at 31,036,147 bp
  • C to A, chromosome 2 at 31,653,871 bp
  • A to T, chromosome 2 at 35,116,798 bp
  • A to T, chromosome 2 at 66,484,298 bp
  • A to T, chromosome 2 at 83,915,607 bp
  • A to C, chromosome 2 at 104,929,556 bp
  • T to C, chromosome 2 at 181,851,659 bp
  • T to C, chromosome 3 at 28,087,189 bp
  • A to G, chromosome 3 at 58,618,928 bp
  • T to C, chromosome 3 at 92,738,021 bp
  • C to T, chromosome 3 at 98,743,898 bp
  • T to C, chromosome 3 at 114,102,572 bp
  • A to G, chromosome 4 at 40,748,600 bp
  • G to A, chromosome 4 at 115,878,072 bp
  • A to T, chromosome 4 at 127,356,162 bp
  • A to G, chromosome 4 at 133,586,045 bp
  • C to T, chromosome 5 at 8,968,869 bp
  • A to G, chromosome 5 at 92,605,557 bp
  • G to A, chromosome 5 at 93,206,557 bp
  • A to G, chromosome 5 at 125,027,038 bp
  • A to T, chromosome 5 at 128,986,417 bp
  • G to T, chromosome 5 at 129,550,593 bp
  • G to T, chromosome 5 at 142,084,491 bp
  • T to C, chromosome 6 at 22,143,974 bp
  • G to T, chromosome 6 at 48,597,345 bp
  • C to A, chromosome 6 at 83,054,795 bp
  • T to A, chromosome 7 at 3,912,996 bp
  • A to T, chromosome 7 at 22,790,300 bp
  • A to G, chromosome 7 at 30,524,135 bp
  • A to G, chromosome 7 at 33,287,498 bp
  • A to T, chromosome 7 at 44,984,136 bp
  • A to G, chromosome 7 at 105,314,447 bp
  • G to T, chromosome 7 at 112,333,839 bp
  • A to T, chromosome 7 at 128,266,395 bp
  • A to G, chromosome 8 at 13,879,990 bp
  • A to G, chromosome 8 at 61,353,753 bp
  • T to C, chromosome 8 at 70,026,723 bp
  • T to A, chromosome 8 at 71,928,189 bp
  • T to C, chromosome 9 at 44,925,973 bp
  • A to T, chromosome 9 at 53,686,400 bp
  • G to A, chromosome 9 at 108,829,978 bp
  • A to C, chromosome 10 at 14,450,577 bp
  • A to C, chromosome 10 at 21,156,425 bp
  • T to C, chromosome 10 at 70,946,993 bp
  • T to G, chromosome 10 at 77,262,470 bp
  • T to C, chromosome 10 at 94,044,916 bp
  • C to T, chromosome 10 at 100,558,176 bp
  • A to T, chromosome 11 at 20,308,587 bp
  • G to A, chromosome 11 at 50,161,620 bp
  • TCACACTCGGCACA to TCACA, chromosome 11 at 50,815,240 bp
  • T to C, chromosome 11 at 71,123,408 bp
  • A to T, chromosome 11 at 103,614,255 bp
  • T to G, chromosome 11 at 104,842,603 bp
  • T to A, chromosome 11 at 111,024,743 bp
  • C to T, chromosome 12 at 16,674,795 bp
  • C to T, chromosome 12 at 17,007,148 bp
  • C to A, chromosome 12 at 32,060,938 bp
  • A to G, chromosome 12 at 118,254,404 bp
  • T to A, chromosome 13 at 14,006,478 bp
  • T to C, chromosome 13 at 18,841,252 bp
  • A to T, chromosome 13 at 23,888,052 bp
  • T to C, chromosome 13 at 54,214,530 bp
  • C to T, chromosome 13 at 62,171,505 bp
  • T to C, chromosome 13 at 73,627,606 bp
  • T to C, chromosome 14 at 32,663,069 bp
  • C to T, chromosome 14 at 54,884,379 bp
  • T to C, chromosome 15 at 58,191,361 bp
  • T to A, chromosome 15 at 94,291,587 bp
  • T to C, chromosome 15 at 102,216,604 bp
  • C to A, chromosome 16 at 16,887,721 bp
  • G to T, chromosome 16 at 22,038,622 bp
  • G to C, chromosome 16 at 45,683,510 bp
  • T to A, chromosome 17 at 14,670,039 bp
  • A to G, chromosome 17 at 35,440,061 bp
  • C to T, chromosome 17 at 38,335,394 bp
  • A to T, chromosome 17 at 83,791,736 bp
  • C to T, chromosome 18 at 36,432,305 bp
  • C to T, chromosome 18 at 37,696,782 bp
  • T to C, chromosome 18 at 37,988,888 bp
  • G to A, chromosome 19 at 4,280,985 bp
  • A to T, chromosome 19 at 18,750,045 bp
  • T to C, chromosome 19 at 24,876,039 bp
  • C to T, chromosome X at 51,130,334 bp
  • A to T, chromosome Y at 725,496 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7747 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045803-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.