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Strain Name:
C57BL/6J-MtgxR7766Btlr/Mmmh
Stock Number:
045822-MU
Citation ID:
RRID:MMRRC_045822-MU
Other Names:
R7766 (G1)
Major Collection:

Strain Information

Ednrb
Name: endothelin receptor type B
Synonyms: ETb, Sox10m1, ETR-b
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 13618
VEGA: 14
HGNC: HGNC:3180
Homologene: 89
Hnrnpab
Name: heterogeneous nuclear ribonucleoprotein A/B
Synonyms: CBF-A, Cgbfa, Hnrpab
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 15384
HGNC: HGNC:5034
Homologene: 74950
Itgb8
Name: integrin beta 8
Synonyms: 4832412O06Rik, D630049N15
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 320910
HGNC: HGNC:6163
Homologene: 18567
Ins2
Name: insulin II
Synonyms: Ins-2, Mody4, Mody, InsII
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 16334
HGNC: HGNC:6081
Homologene: 173
Kcna6
Name: potassium voltage-gated channel, shaker-related, subfamily, member 6
Synonyms: Kv1.6, MK1.6
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 16494
HGNC: HGNC:6225
Homologene: 1684
Slc17a6
Name: solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6
Synonyms: 2900073D12Rik, VGLUT2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 140919
Homologene: 121617
Usp28
Name: ubiquitin specific peptidase 28
Synonyms: 9830148O20Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235323
VEGA: 9
Homologene: 10840
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 149,861,058 bp
  • A to T, chromosome 1 at 159,888,310 bp
  • T to A, chromosome 2 at 25,441,528 bp
  • T to C, chromosome 2 at 76,814,698 bp
  • T to A, chromosome 2 at 85,785,658 bp
  • T to C, chromosome 2 at 103,725,707 bp
  • T to C, chromosome 2 at 122,337,301 bp
  • G to A, chromosome 2 at 174,426,832 bp
  • T to C, chromosome 4 at 140,730,975 bp
  • T to A, chromosome 4 at 143,699,239 bp
  • T to A, chromosome 4 at 147,869,270 bp
  • A to T, chromosome 5 at 21,561,044 bp
  • T to C, chromosome 5 at 76,317,896 bp
  • A to T, chromosome 5 at 87,001,202 bp
  • G to T, chromosome 5 at 114,937,110 bp
  • A to G, chromosome 5 at 145,718,017 bp
  • A to G, chromosome 6 at 29,496,847 bp
  • A to T, chromosome 6 at 48,844,182 bp
  • C to T, chromosome 6 at 85,512,199 bp
  • T to C, chromosome 6 at 121,638,341 bp
  • T to A, chromosome 6 at 126,739,719 bp
  • A to T, chromosome 7 at 8,368,220 bp
  • A to G, chromosome 7 at 24,104,659 bp
  • A to T, chromosome 7 at 51,669,166 bp
  • T to A, chromosome 7 at 104,106,994 bp
  • C to T, chromosome 7 at 108,630,376 bp
  • C to T, chromosome 7 at 130,743,598 bp
  • T to A, chromosome 7 at 142,678,757 bp
  • T to C, chromosome 8 at 22,653,091 bp
  • T to C, chromosome 9 at 49,035,883 bp
  • A to T, chromosome 10 at 60,004,819 bp
  • G to A, chromosome 11 at 51,601,466 bp
  • A to C, chromosome 11 at 67,358,329 bp
  • C to G, chromosome 11 at 103,139,012 bp
  • T to C, chromosome 12 at 119,163,359 bp
  • C to T, chromosome 13 at 97,150,400 bp
  • T to C, chromosome 13 at 114,853,891 bp
  • T to C, chromosome 14 at 57,447,654 bp
  • A to G, chromosome 14 at 103,843,289 bp
  • T to C, chromosome 15 at 8,296,849 bp
  • A to G, chromosome 15 at 89,161,271 bp
  • A to T, chromosome 16 at 25,868,219 bp
  • A to T, chromosome 16 at 96,790,901 bp
  • A to T, chromosome 17 at 33,138,423 bp
  • G to T, chromosome 17 at 46,737,235 bp
  • A to T, chromosome 19 at 5,456,852 bp
  • A to C, chromosome 19 at 11,449,988 bp
  • GCGAACG to GCG, chromosome 19 at 25,411,205 bp
  • A to G, chromosome X at 83,487,098 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7766 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045822-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.