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Strain Name:
C57BL/6J-MtgxR7772Btlr/Mmmh
Stock Number:
045828-MU
Citation ID:
RRID:MMRRC_045828-MU
Other Names:
R7772 (G1)
Major Collection:

Strain Information

Nedd4
Name: neural precursor cell expressed, developmentally down-regulated 4
Synonyms: Nedd4-1, Nedd4, Nedd4a, E430025J12Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 17999
HGNC: HGNC:7727
Homologene: 134533
Slc12a8
Name: solute carrier family 12 (potassium/chloride transporters), member 8
Synonyms: E330020C02Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 171286
Homologene: 11628
Dst
Name: dystonin
Synonyms: bullous pemphigoid antigen 1, BPAG1-n, BPAG1, Bpag1, Bpag, ah, bullous pemphigoid antigen 1, athetoid, Macf2, 2310001O04Rik, nmf203, A830042E19Rik, nmf339
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13518
HGNC: HGNC:1090
Homologene: 136716
Col18a1
Name: collagen, type XVIII, alpha 1
Synonyms: endostatin
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 12822
HGNC: HGNC:2195
Homologene: 7673
Nup210l
Name: nucleoporin 210-like
Synonyms: 4930548O11Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 77595
Homologene: 28122
Parp1
Name: poly (ADP-ribose) polymerase family, member 1
Synonyms: PARP, sPARP-1, Adprp, parp-1, 5830444G22Rik, Adprt1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 11545
HGNC: HGNC:270
Homologene: 1222
Cobl
Name: cordon-bleu WH2 repeat
Synonyms: C530045F18Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 12808
Homologene: 9058
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 10,157,010 bp
  • T to C, chromosome 1 at 34,181,388 bp
  • G to A, chromosome 1 at 74,526,992 bp
  • G to T, chromosome 1 at 125,677,597 bp
  • G to T, chromosome 1 at 133,170,022 bp
  • A to T, chromosome 1 at 173,481,074 bp
  • G to T, chromosome 1 at 180,589,398 bp
  • T to C, chromosome 2 at 76,657,533 bp
  • C to A, chromosome 2 at 129,125,544 bp
  • A to G, chromosome 2 at 131,189,109 bp
  • A to G, chromosome 2 at 145,930,644 bp
  • C to T, chromosome 2 at 176,720,971 bp
  • A to G, chromosome 3 at 90,159,926 bp
  • T to C, chromosome 3 at 100,080,118 bp
  • T to C, chromosome 3 at 105,907,723 bp
  • T to G, chromosome 3 at 146,433,130 bp
  • A to T, chromosome 4 at 11,227,190 bp
  • T to A, chromosome 4 at 52,970,713 bp
  • A to T, chromosome 4 at 71,756,581 bp
  • A to C, chromosome 4 at 95,050,844 bp
  • T to C, chromosome 4 at 109,066,187 bp
  • T to C, chromosome 4 at 133,411,965 bp
  • T to A, chromosome 4 at 148,164,326 bp
  • A to T, chromosome 5 at 65,080,174 bp
  • A to T, chromosome 5 at 92,403,613 bp
  • A to T, chromosome 5 at 128,739,463 bp
  • T to C, chromosome 6 at 23,390,446 bp
  • C to A, chromosome 7 at 5,115,544 bp
  • T to C, chromosome 7 at 11,162,843 bp
  • T to C, chromosome 7 at 12,418,608 bp
  • A to C, chromosome 7 at 44,515,924 bp
  • T to G, chromosome 7 at 103,086,181 bp
  • A to T, chromosome 7 at 126,448,535 bp
  • A to G, chromosome 7 at 127,905,901 bp
  • C to T, chromosome 8 at 13,248,682 bp
  • T to C, chromosome 9 at 30,428,925 bp
  • T to C, chromosome 9 at 40,127,365 bp
  • T to C, chromosome 9 at 72,677,326 bp
  • A to T, chromosome 10 at 33,921,702 bp
  • T to A, chromosome 10 at 77,068,386 bp
  • A to T, chromosome 10 at 77,561,112 bp
  • T to A, chromosome 11 at 3,889,784 bp
  • C to T, chromosome 11 at 12,254,488 bp
  • T to A, chromosome 11 at 83,429,277 bp
  • G to T, chromosome 11 at 99,389,087 bp
  • T to C, chromosome 11 at 116,221,435 bp
  • T to C, chromosome 12 at 17,348,585 bp
  • A to T, chromosome 12 at 71,061,589 bp
  • T to A, chromosome 12 at 109,593,185 bp
  • A to G, chromosome 13 at 11,751,011 bp
  • T to C, chromosome 13 at 12,417,641 bp
  • T to C, chromosome 13 at 13,359,406 bp
  • T to A, chromosome 13 at 22,806,494 bp
  • T to A, chromosome 14 at 45,343,650 bp
  • T to A, chromosome 14 at 56,477,687 bp
  • A to T, chromosome 14 at 66,828,976 bp
  • T to C, chromosome 15 at 9,704,481 bp
  • C to A, chromosome 15 at 10,958,044 bp
  • CTCTTCTTCTTCTTC to CTCTTCTTCTTC, chromosome 16 at 10,790,701 bp
  • G to A, chromosome 16 at 33,550,965 bp
  • A to G, chromosome 16 at 34,031,582 bp
  • A to G, chromosome 16 at 36,277,001 bp
  • G to T, chromosome 16 at 43,762,395 bp
  • T to C, chromosome 16 at 73,961,889 bp
  • A to T, chromosome 17 at 13,109,752 bp
  • A to G, chromosome 17 at 18,313,220 bp
  • T to A, chromosome 17 at 25,861,491 bp
  • A to C, chromosome 17 at 56,512,247 bp
  • C to T, chromosome 17 at 80,273,078 bp
  • G to T, chromosome 18 at 38,309,576 bp
  • T to A, chromosome 18 at 90,527,794 bp
  • T to C, chromosome 19 at 5,365,122 bp
  • T to A, chromosome 19 at 34,117,891 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7772 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045828-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.