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Strain Name:
C57BL/6J-MtgxR7775Btlr/Mmmh
Stock Number:
045831-MU
Citation ID:
RRID:MMRRC_045831-MU
Other Names:
R7775 (G1)
Major Collection:

Strain Information

Chrna6
Name: cholinergic receptor, nicotinic, alpha polypeptide 6
Synonyms: Acra6, alpha6 nAChR
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 11440
Homologene: 20888
Tfap2b
Name: transcription factor AP-2 beta
Synonyms: AP-2(beta), E130018K07Rik, Tcfap2b
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 21419
Homologene: 20688
Txndc2
Name: thioredoxin domain containing 2 (spermatozoa)
Synonyms: Sptrx-1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 213272
VEGA: 17
Homologene: 41856
Lrp2
Name: low density lipoprotein receptor-related protein 2
Synonyms: Megalin, Gp330, D230004K18Rik, b2b1625.2Clo
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14725
HGNC: HGNC:6694
Homologene: 20952
Dtx4
Name: deltex 4, E3 ubiquitin ligase
Synonyms: RNF155
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 207521
VEGA: 19
Homologene: 27781
Dyrk3
Name: dual-specificity tyrosine phosphorylation regulated kinase 3
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226419
HGNC: HGNC:3094
Homologene: 55762
Arhgap35
Name: Rho GTPase activating protein 35
Synonyms: P190 RhoGAP, 6430596G11Rik, p190RhoGAP, p190A, Grlf1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 232906
HGNC: HGNC:4591
Homologene: 35136
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 19,234,307 bp
  • A to T, chromosome 1 at 20,979,461 bp
  • A to T, chromosome 1 at 36,348,677 bp
  • G to A, chromosome 1 at 59,123,513 bp
  • A to G, chromosome 1 at 86,543,528 bp
  • G to T, chromosome 1 at 93,940,784 bp
  • C to T, chromosome 1 at 131,129,627 bp
  • T to A, chromosome 1 at 178,864,876 bp
  • A to G, chromosome 2 at 24,435,901 bp
  • C to T, chromosome 2 at 53,073,680 bp
  • T to A, chromosome 2 at 69,501,539 bp
  • A to G, chromosome 2 at 91,044,948 bp
  • A to G, chromosome 2 at 112,479,956 bp
  • T to A, chromosome 2 at 155,024,710 bp
  • C to T, chromosome 4 at 46,637,746 bp
  • A to T, chromosome 4 at 126,051,377 bp
  • A to G, chromosome 5 at 124,641,505 bp
  • C to T, chromosome 5 at 137,641,924 bp
  • T to A, chromosome 5 at 140,523,621 bp
  • C to T, chromosome 5 at 145,104,812 bp
  • T to C, chromosome 6 at 27,363,672 bp
  • A to G, chromosome 6 at 29,177,719 bp
  • A to T, chromosome 6 at 126,534,805 bp
  • T to C, chromosome 7 at 16,562,648 bp
  • A to G, chromosome 7 at 43,190,296 bp
  • A to T, chromosome 7 at 46,926,352 bp
  • A to G, chromosome 8 at 11,561,814 bp
  • T to C, chromosome 8 at 27,407,364 bp
  • G to T, chromosome 8 at 46,586,424 bp
  • A to C, chromosome 8 at 54,584,792 bp
  • A to T, chromosome 8 at 94,089,760 bp
  • A to T, chromosome 8 at 95,716,238 bp
  • A to G, chromosome 9 at 20,282,412 bp
  • T to A, chromosome 9 at 38,026,667 bp
  • T to A, chromosome 9 at 38,994,907 bp
  • C to A, chromosome 9 at 39,669,238 bp
  • A to T, chromosome 10 at 26,352,317 bp
  • A to T, chromosome 10 at 39,078,847 bp
  • C to T, chromosome 11 at 53,176,971 bp
  • T to G, chromosome 11 at 77,465,773 bp
  • T to C, chromosome 11 at 84,965,623 bp
  • A to G, chromosome 11 at 98,851,154 bp
  • T to G, chromosome 11 at 116,849,270 bp
  • A to T, chromosome 13 at 58,251,152 bp
  • A to G, chromosome 14 at 4,986,951 bp
  • A to G, chromosome 14 at 20,842,325 bp
  • A to G, chromosome 14 at 31,352,410 bp
  • A to G, chromosome 14 at 73,029,763 bp
  • T to C, chromosome 14 at 79,038,147 bp
  • A to T, chromosome 15 at 34,399,077 bp
  • T to C, chromosome 15 at 75,918,289 bp
  • A to T, chromosome 15 at 76,176,935 bp
  • T to C, chromosome 15 at 81,586,686 bp
  • G to T, chromosome 16 at 21,958,914 bp
  • A to T, chromosome 16 at 59,303,251 bp
  • G to T, chromosome 17 at 12,464,463 bp
  • T to A, chromosome 17 at 23,681,607 bp
  • T to C, chromosome 17 at 46,910,806 bp
  • T to C, chromosome 17 at 57,525,942 bp
  • A to G, chromosome 17 at 65,638,243 bp
  • A to T, chromosome 18 at 37,012,680 bp
  • A to G, chromosome 18 at 37,695,525 bp
  • G to T, chromosome 18 at 68,235,669 bp
  • T to C, chromosome 18 at 68,235,756 bp
  • G to T, chromosome 19 at 12,492,010 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7775 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045831-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.