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Strain Name:
C57BL/6J-MtgxR7776Btlr/Mmmh
Stock Number:
045832-MU
Citation ID:
RRID:MMRRC_045832-MU
Other Names:
R7776 (G1)
Major Collection:

Strain Information

Rptor
Name: regulatory associated protein of MTOR, complex 1
Synonyms: raptor, 4932417H02Rik, Rap
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 74370
Homologene: 80210
Nucb2
Name: nucleobindin 2
Synonyms: NEFA, Calnuc, nesfatin-1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 53322
HGNC: HGNC:8044
Homologene: 3676
Etl4
Name: enhancer trap locus 4
Synonyms: Etl-4, E330027G05Rik, 6620402G01Rik, 9430077C05Rik, Skt, Sickle tail
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 208618
Homologene: 10477
Ptpn23
Name: protein tyrosine phosphatase, non-receptor type 23
Synonyms: PTP-TD14
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 104831
Homologene: 135706
Aspm
Name: abnormal spindle microtubule assembly
Synonyms: Sha1, Aspm, D330028K02Rik, MCPH5, Calmbp1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12316
Homologene: 7650
Rnf20
Name: ring finger protein 20
Synonyms: 4833430L21Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 109331
Homologene: 5571
Slc38a2
Name: solute carrier family 38, member 2
Synonyms: 5033402L14Rik, SNAT2
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 67760
VEGA: 15
Homologene: 23132
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 139,479,846 bp
  • T to A, chromosome 1 at 165,352,054 bp
  • A to T, chromosome 2 at 20,807,146 bp
  • T to C, chromosome 2 at 52,207,701 bp
  • T to A, chromosome 2 at 84,840,853 bp
  • T to C, chromosome 2 at 88,224,085 bp
  • C to A, chromosome 2 at 129,125,544 bp
  • T to C, chromosome 2 at 177,921,739 bp
  • A to G, chromosome 3 at 83,766,779 bp
  • A to T, chromosome 3 at 87,444,195 bp
  • C to T, chromosome 4 at 49,644,592 bp
  • T to A, chromosome 4 at 115,032,838 bp
  • T to A, chromosome 4 at 140,575,331 bp
  • T to A, chromosome 4 at 140,924,345 bp
  • T to C, chromosome 5 at 86,533,746 bp
  • T to C, chromosome 5 at 138,182,020 bp
  • T to A, chromosome 6 at 21,836,443 bp
  • A to G, chromosome 6 at 91,269,199 bp
  • AAGTA to AAGTACAGTA, chromosome 6 at 113,399,159 bp
  • T to C, chromosome 6 at 142,764,557 bp
  • T to C, chromosome 7 at 3,823,247 bp
  • T to A, chromosome 7 at 5,454,635 bp
  • T to A, chromosome 7 at 12,418,642 bp
  • T to A, chromosome 7 at 64,248,191 bp
  • T to A, chromosome 7 at 108,925,449 bp
  • T to A, chromosome 7 at 116,529,013 bp
  • T to C, chromosome 7 at 120,232,991 bp
  • A to G, chromosome 7 at 141,704,393 bp
  • A to T, chromosome 8 at 12,880,127 bp
  • C to T, chromosome 8 at 13,248,682 bp
  • T to A, chromosome 8 at 54,077,800 bp
  • T to C, chromosome 8 at 76,909,545 bp
  • A to T, chromosome 9 at 44,974,161 bp
  • A to T, chromosome 9 at 73,694,950 bp
  • G to A, chromosome 9 at 110,386,300 bp
  • G to A, chromosome 9 at 119,380,700 bp
  • A to G, chromosome 9 at 120,558,502 bp
  • G to T, chromosome 10 at 80,918,157 bp
  • A to T, chromosome 10 at 92,868,741 bp
  • A to G, chromosome 11 at 69,406,134 bp
  • A to G, chromosome 11 at 75,190,446 bp
  • A to G, chromosome 11 at 78,209,529 bp
  • T to C, chromosome 11 at 96,934,564 bp
  • A to G, chromosome 11 at 100,578,988 bp
  • T to C, chromosome 11 at 119,892,627 bp
  • G to A, chromosome 12 at 71,317,914 bp
  • T to A, chromosome 12 at 80,492,560 bp
  • A to G, chromosome 12 at 87,431,445 bp
  • A to T, chromosome 13 at 23,585,955 bp
  • G to A, chromosome 14 at 55,865,963 bp
  • T to C, chromosome 15 at 96,690,152 bp
  • A to G, chromosome 15 at 101,053,420 bp
  • T to C, chromosome 16 at 3,900,709 bp
  • C to A, chromosome 16 at 45,667,651 bp
  • A to T, chromosome 17 at 18,823,672 bp
  • T to A, chromosome 17 at 21,691,959 bp
  • T to A, chromosome 17 at 24,386,276 bp
  • T to A, chromosome 17 at 70,851,457 bp
  • G to A, chromosome 17 at 73,322,054 bp
  • T to A, chromosome 18 at 36,969,981 bp
  • T to A, chromosome 18 at 37,732,954 bp
  • T to A, chromosome 19 at 7,021,773 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R7776 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
045832-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.