Strain Name:
STOCK Tg(Nefh-cre)12Kul/Mmucd
Stock Number:
000049-UCD
Citation ID:
RRID:MMRRC_000049-UCD

Strain Information

Nefh
Name: neurofilament, heavy polypeptide
Synonyms: NF200, NEFH, NF-H
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 380684
HGNC: HGNC:7737
Homologene: 40755
cre
Name: cre recombinase
Type: Gene
Species: Enterobacteria phage P1 (bacteriophage P1)
Chromosome:
Tg(Nefh-cre)12Kul
Name: transgene insertion 12, Ashok B Kulkarni
Synonyms: Tg mNF-H-cre 12
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic (cre)
Genetic Alterations
The transgenic vector consists of the cre recombinase gene, including its ATG, fused to DNA segments for the nuclear localization signal from simian virus 40 (SV40) large T antigen and the SV40 poly A signal, all under the control of the mNF-H promoter region. The promoter region of the mNF-H gene used is an 8.5 kb fragment containing the 5 -UTR and 40 bp of non-coding sequence of exon 1 ending before the ATG.
Genotype Determination
Phenotype
Neuron-specific Cre expression in the brain and spinal cord during late-stage development (E18.5).

NOTE: The MMRRC at UC-Davis has observed possible seizure and abnormal gait phenotypes in one or more male mice from hemizygous x hemizygous matings. The facility reports that seizures have not been observed in mice from hemizygous x wild-type matings.
MeSH Terms
  • Age Factors
  • Animals
  • Animals, Newborn
  • Brain/embryology
  • Brain/enzymology
  • Brain/growth & development
  • Cell Differentiation/genetics
  • Fetus
  • Galactosides/pharmacokinetics
  • Gene Deletion
  • Gene Expression Regulation, Developmental/genetics
  • Gene Targeting
  • Genes, Reporter/physiology
  • Indoles/pharmacokinetics
  • Integrases/genetics
  • Integrases/metabolism
  • Mice
  • Mice, Transgenic/embryology
  • Mice, Transgenic/genetics
  • Mice, Transgenic/growth & development
  • Neurofilament Proteins/genetics
  • Neurons/enzymology
  • Promoter Regions, Genetic/physiology
  • Transgenes/genetics
  • Viral Proteins
Strain Development
The transgenic vector was injected into the pronuclei of fertilized oocytes of FVB/N mice to create transgenic founder mice. This strain was originally maintained by sibling inbreeding from the initial crossing of (FVB/N x C57BL/6 x 129X1/SvJ). Since the transfer to the MMRRC, this strain has been further backcrossed to C57BL/6J to produce the congenic strain, B6J.Cg-Tg(Nfh-cre)12Kul/Mmcd, MMRRC:015960, that is 99% C57BL/6J by microsatellite testing.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Make neuron-specific conditional KO lines
  • Neurology
Donor
Ashok Kulkarni, National Institute of Dental and Craniofacial Research, National Institutes of Health.
Primary Reference

Hirasawa M, Cho A, Sreenath T, Sauer B, Julien JP, Kulkarni AB. Neuron-specific expression of Cre recombinase during the late phase of brain development. Neurosci Res. 2001 Jun;40(2):125-32. (Medline PMID: 11377750)

Colony and Husbandry Information

The donating investigator reports that mNFHcre mouse line 12 homozygotes develop a neurological phenotype with seizures and become infertile and moribund.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Coat Color
Agouti, Black or White
MMRRC Breeding System
Backcross

Note: Since Hemizygous x Hemizygous matings may produce mice with a seizure disorder phenotype, this breeding scheme is no longer recommended (Aug. 7, 2003).
Breeding Scheme(s)
  • Wild-type Female x Hemizygous Transgenic Male
  • Hemizygous Transgenic Female x Wild-type Male
Generation
F?
Overall Breeding Performance
Good
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Average litter size
8
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
000049-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
000049-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
000049-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.