Strain Name:
STOCK Plntm1Egk Tg(Myh6-Pln)11Egk/Mmmh
Stock Number:
000052-MU
Citation ID:
RRID:MMRRC_000052-MU
Other Names:
STOCK Plntm1Egk Tg(Myh6-Pln*S16A)11Egk/Mmmh

Strain Information

Plntm1Egk
Name: phospholamban; targeted mutation 1, Evangelia G Kranias
Synonyms: PLB-, PLN-, PLB-KO
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Knockout
Pln
Name: phospholamban
Synonyms: PLB
Type: Gene
Species: Mouse
Chromosome: 10
Alteration at locus: Transgenic
NCBI: 18821
VEGA: 10
HGNC: HGNC:9080
Homologene: 2002
Tg(Myh6-Pln)11Egk
Name: transgene insertion 11, Evangelia G Kranias
Synonyms: Ser16--Ala mutant PLB, S16A mutant PLB, S16A PLB, Tg(Pln)11Egk
Type: Allele
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic
Myh6
Name: myosin, heavy polypeptide 6, cardiac muscle, alpha
Synonyms: alphaMHC, alpha cardiac MHC, A830009F23Rik, cardiomyopathy, hypertrophic 1, Myhc-a, Myhca, alpha-MHC, alpha myosin
Type: Gene
Species: Mouse
Chromosome: 14
Alteration at locus: Transgenic
NCBI: 17888
HGNC: HGNC:7576
Homologene: 124414
Genetic Alterations
Neo cassette in exon 2 of the endogenous mouse phospholamban gene along with a mouse phospholamban mutant (S16A) transgene under the control of the murine alpha-myosin heavy chain 6 promoter.

HGVS nomenclature:

Genotype Determination
ES Cell Line
D3 derived from 129S2/SvPas
Phenotype
Cardiac specific overexpression of homozygous transgene mutant (serine 16 to alanine) phospholamban. This model exhibits no cardiac functional alterations under basal conditions. However, it displays significantly attenuated responses to beta-adrenergic stimulation.
Mammalian Phenotype Terms
Allelic Composition: Plntm1Egk/Plntm1Egk (Genetic Background: involves: 129S2/SvPas )

Allelic Composition: Plntm1Egk/Plntm1Egk (Genetic Background: involves: 129S2/SvPas * CF-1 * FVB/N )

Allelic Composition: Plntm1Egk/Plntm1Egk (Genetic Background: involves: 129S2/SvPas * CF1 )

MeSH Terms
  • Amino Acid Substitution
  • Animals
  • Calcium/metabolism
  • Calcium-Binding Proteins/chemistry
  • Calcium-Binding Proteins/genetics
  • Calcium-Binding Proteins/metabolism
  • Calcium-Transporting ATPases/metabolism
  • Heart/drug effects
  • Heart/physiology
  • Isoproterenol/pharmacology
  • Kinetics
  • Mice
  • Mice, Knockout
  • Mice, Transgenic
  • Mutagenesis, Site-Directed
  • Mutation, Missense
  • Myocardium/metabolism
  • Myosin Heavy Chains/genetics
  • Phosphorylation
  • Promoter Regions, Genetic
  • Receptors, Adrenergic, beta/physiology
  • Sarcoplasmic Reticulum Calcium-Transporting ATPases
  • Serine
Strain Development
A mouse cardiac phospholamban mutant (S16A) cDNA transgene driven by the alpha myosin heavy chain promoter was microinjected into the pronuclei of FVB/N zygotes. Transgene positive founders were mated to the homozygous phospholamban knockout mice (see MMRRC:000027). Resultant offspring were mated to the homozygous phospholamban knockout mice again in order to produce offspring that were homozygous for the phospholamban knockout gene and hemizygous for the phospholamban mutant transgene. The strain was then maintained by brother sister mating of transgene negative mice to transgene positive mice in order to maintain homozygous phospholamban knockout mice which are hemizygous for the phospholamban mutant transgene.
Suggested Control Mice
  • Homozygous phospholamban knockout littermates
  • MMRRC:000027
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Cardiovascular Research
  • Beta adrenergic stimulation of the heart is important especially during exercise and stress to enable the heart to beat more forcefully and perfuse all the organs of the body. The beta agonists mediate this effect through activation of cAMP-dependent protein kinase (PKA) and phosphorylation of cardiac proteins, including phospholamban. In this model, phospholamban cannot become phosphorylated, and as a result, the beta agonist stimulatory effects are significantly attenuated, indicating that phospholamban is a key mediator of beta adrenergic stimulation in vivo. This mouse will be a valuable model to dissect: the mechanisms of the stimulatory effects of beta agonists in the healthy heart; and the involvement of the beta adrenergic system in heart disease, where it has been implicated to play a major role.
Donor
Dr. Evangelia Kranias, University of Cincinnati College of Medicine
Primary Reference

Chu G, Lester JW, Young KB, Luo W, Zhai J, Kranias EG. A single site (Ser16) phosphorylation in phospholamban is sufficient in mediating its maximal cardiac responses to beta-agonists. J Biol Chem. 2000 Dec 8; 275(49):38938-43. (Medline PMID: 10988285)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Agouti
Eye
Black
MMRRC Breeding System
Backcross and sib-mating
Breeding Scheme(s)
  • Transgenic male or female in the phospholamban knockout background are crossed with phospholamban knockout female or male mice (-/- littermates or MMRRC:000027).
  • Homozygous KO Female x Hemizygous Tg Male
  • Hemizygous Tg Female x Homozygous KO Male
Generation
N10+ (MMRRC:27)
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Unknown Unknown
Average litter size
5
Recommended wean age
3 weeks
Average Pups Weaned
5

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
000052-MU-SPERM Cryo-preserved spermatozoa $437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
000052-MU-RESUS Litter recovered from cryo-archive $2,016.00 / $4,103.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.