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Strain Detail Sheet

Strain Name:
B6J.B6N-Rhcgtm1Idw/IdwMmmh
Stock Number:
066712-MU
Citation ID:
RRID:MMRRC_066712-MU
Other Names:
Floxed Rhcg

Strain Information

Gene Details

Rhcgtm1Idw
Name: Rhesus blood group-associated C glycoprotein; targeted mutation 1, I David Weiner
Synonyms: Rhcgfl
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Conditional
Rhcg
Name: Rhesus blood group-associated C glycoprotein
Type: Gene
Species: Mouse
Chromosome: 7
Alteration at locus: Conditional
NCBI: 56315
Homologene: 32310

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
LoxP sites were inserted around a 2.7-kb region including exons 5 - 9, and a floxed neo cassette was inserted via homologous recombination.
ES Cell Line
C57BL/6NTac-derived ESC line

Strain Description

Phenotype
None/Normal/Wild-type. Decreased urinary ammonia excretion upon Cre modification.
MeSH Terms
  • Animals
  • Cation Transport Proteins/genetics
  • Cation Transport Proteins/immunology
  • Cation Transport Proteins/metabolism
  • Cell Membrane/metabolism
  • Immunohistochemistry
  • Kidney/metabolism
  • Kidney/ultrastructure
  • Membrane Glycoproteins/genetics
  • Membrane Glycoproteins/immunology
  • Membrane Glycoproteins/metabolism
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C3H
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Microscopy, Electron, Transmission
  • Protein Isoforms/metabolism
  • Acidosis/metabolism
  • Ammonia/urine
  • Gene Expression Regulation/physiology
  • Hydrogen-Ion Concentration
  • Kidney/drug effects
  • Mice, Knockout
  • Time Factors
Strain Development
Mouse C57BL/6 BAC clone RP23-207N5 was used to generate the homologous arms and the conditional knockout region for the gene-targeting vector and the Southern probes for screening targeted events. The 5’ homologous arm (8.1 kb) and conditional knockout region (2.7 kb) were generated by RED cloning/gap repair. The 3’ homologous arm (1.7 kb) was generated by PCR using proofreading LA Taq DNA polymerase (Takara). These fragments were cloned into the 3loxP3NwCD vector and confirmed by restriction digestion and end-sequencing. The final vector was obtained by standard molecular cloning. Aside from the homologous arms, the final vector also contained loxP sequences flanking the conditional knockout region (2.7 kb), loxP sequences flanking the Neo expression cassette [for positive selection of the embryonic stem (ES) cells], and a DTA expression cassette (for negative expression of the ES cells). The final vector was confirmed by both restriction digestion and end-sequencing analysis. NotI was used for linearizing the final vector before electroporation. External probes (5’ and 3’) were generated by PCR reaction and tested on genomic Southern analysis for ES screening. They were cloned into a pCR2.1 backbone and were confirmed by sequencing. Subsequently, ES cells derived from C57BL/6NTac were transfected with the gene-targeting construct, and homologous recombinant clones were identified. Homologous recombination ES clones were microinjected into blastocysts and transferred into pseudopregnant females to generate chimeras. Chimeras were bred to generate hemizygous floxed Rhcg mice, and the hemizygous mice were used to generate homozygous floxed Rhcg mice. This work was done by Caliper Life Sciences (formerly Xenogen Biosciences) under contract from the University of Florida. Subsequently, the mice were rederived using IVF techniques and cross-bred with C57BL/6J mice for 10 generations, followed by interbreeding for several more years prior to donation to the MMRRC.
Suggested Control Mice
C57BL/6J

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.

Research Applications

  • Metabolism

Strain Origin

Donor
I. David Weiner, M.D., University of Florida, Department of Medicine.
Primary Reference

Kim HY, Verlander JW, Bishop JM, Cain BD, Han KH, Igarashi P, Lee HW,Handlogten ME, Weiner ID. Basolateral expression of the ammonia transporterfamily member Rh C glycoprotein in the mouse kidney. Am J Physiol Renal Physiol. 2009 Mar;296(3):F543-55. doi: 10.1152/ajprenal.90637.2008. Epub 2009 Jan 7.(Medline PMID: 19129254)

Lee HW, Verlander JW, Bishop JM, Igarashi P, Handlogten ME, Weiner ID.Collecting duct-specific Rh C glycoprotein deletion alters basal andacidosis-stimulated renal ammonia excretion. Am J Physiol Renal Physiol. 2009Jun;296(6):F1364-75. doi: 10.1152/ajprenal.90667.2008. Epub 2009 Mar 25. (Medline PMID: 19321595)

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
Black
Eye
Black

Breeding

MMRRC Breeding System
Backcross then sib-mating
Generation
N10 (C57BL/6J), F20+
Overall Breeding Performance
Excellent

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
7 to 9
Recommended wean age
3 Weeks
Average Pups Weaned
7 to 9

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
066712-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
066712-MU-RESUS Litter recovered from cryo-archive $2,624.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.