Loading...

Strain Detail Sheet

Strain Name:
STOCK Ppp1r8tm1.1Itl/MbolMmmh
Stock Number:
067024-MU
Citation ID:
RRID:MMRRC_067024-MU
Other Names:
Ppp1r8 fl/fl mice

Strain Information

Gene Details

Ppp1r8tm1.1Itl
Name: protein phosphatase 1, regulatory subunit 8; targeted mutation 1.1, inGenious Targeting Laboratory
Synonyms: Ppp1r8fl
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Conditional
Ppp1r8
Name: protein phosphatase 1, regulatory subunit 8
Synonyms: 6330548N22Rik, NIPP1
Type: Gene
Species: Mouse
Chromosome: 4
Alteration at locus: Conditional
NCBI: 100336
HGNC: HGNC:9296
Homologene: 8555

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
Conditional-ready knockout of the Ppp1r8 gene by floxing a 3.74-kb gene region spanning from the promoter through exon 2 and introduction of a neomycin resistance cassette.
ES Cell Line
iTL BA1 derived from (C57BL/6NTac x 129S6/SvEvTac)F1

Strain Description

Phenotype
None/Normal/Wild-type.

Conditional phenotype:

  • Transgenic mice Tg(Alfp-Cre) expressing Cre recombinase under the control of Alfp promoter to delete Ppp1r8 in epithelial cells of the liver. The liver-specific deletion of Ppp1r8 causes biliary epithelial cell hyperproliferation, including progenitor cells. (PMID:27068806)
  • Transgenic mice Tg(Krt14-Cre) expressing Cre recombinase under the control of Keratin-14 promoter to delete Ppp1r8 in keratinocytes. Selective deletion of Ppp1r8 in mouse epidermis results in epidermal hyperproliferation, a reduced adherence of basal keratinocytes, and a gradual decrease in the stemness of hair follicle stem cells, culminating in hair loss. This complex phenotype is associated with chronic chemokine-driven skin inflammation. (PMID:31972250)
  • Transgenic mice Tg(UBC-Cre-ERT2) expressing tamoxifen-activated Cre-ERT2 recombinase under the control of the UBC promoter. The induced inactivation of Ppp1r8-encoding alleles in adult mice occurred very efficiently in the testes and resulted in a gradual loss of germ cells, culminating in a Sertoli-only phenotype (PMID:29042623).
Strain Development
The conditional Ppp1r8 knockout mouse was engineered by inGenious Targeting Laboratory, Inc. in iTL BA1 ES cell line derived from (C57BL/6NTac x 129S6/SvEvTac)F1. Resulting chimeras with a high percentage of agouti coat color were mated to wild-type C57BL/6N mice to generate F1 heterozygous offspring. The neo cassette was subsequently removed by crossing with 129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym/J (RRID:IMSR_JAX:003946).
Suggested Control Mice
Wild-type littermates

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.

Research Applications

  • Cell Biology
  • Dermatology
  • Immunology and Inflammation
  • Models for Human Disease
  • Reproduction

Strain Origin

Donor
Mathieu Bollen, Ph.D., KU Leuven.
Primary Reference

Verbinnen I, Jonkhout M, Liakath-Ali K, et al. Phosphatase Regulator NIPP1 Restrains Chemokine-Driven Skin Inflammation [published online ahead of print, 2020 Jan 21]. J Invest Dermatol. 2020;S0022-202X(20)30035-X. doi:10.1016/j.jid.2020.01.008

Verbinnen I, Boens S, Ferreira M, et al. Enhanced DNA-repair capacity and resistance to chemically induced carcinogenesis upon deletion of the phosphatase regulator NIPP1. Oncogenesis. 2020;9(3):30. Published 2020 Mar 2. doi:10.1038/s41389-020-0214-3

Ferreira M, Verbinnen I, Fardilha M, Van Eynde A, Bollen M. The deletion of the protein phosphatase 1 regulator NIPP1 in testis causes hyperphosphorylation and degradation of the histone methyltransferase EZH2. J Biol Chem. 2018;293(47):18031–18039. doi:10.1074/jbc.AC118.005577

Ferreira M, Boens S, Winkler C, et al. The protein phosphatase 1 regulator NIPP1 is essential for mammalian spermatogenesis. Sci Rep. 2017;7(1):13364. Published 2017 Oct 17. doi:10.1038/s41598-017-13809-y

Boens S, Verbinnen I, Verhulst S, et al. Brief Report: The Deletion of the Phosphatase Regulator NIPP1 Causes Progenitor Cell Expansion in the Adult Liver. Stem Cells. 2016;34(8):2256–2262. doi:10.1002/stem.2375

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
Brown
Eye
Black

Breeding

MMRRC Breeding System
Random intra-strain mating
Generation
F?
Overall Breeding Performance
Excellent

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 10 to 12 months Greater than 12 months
Average litter size
7 to 9
Recommended wean age
3 Weeks
Average Pups Weaned
7 to 9

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
067024-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
067024-MU-RESUS Litter recovered from cryo-archive $2,624.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.