Strain Name:
BALB/cByJ-mtNZB/BlNJ;BALB/cByJ/ShoueCmtMmmh
Stock Number:
067057-MU
Citation ID:
RRID:MMRRC_067057-MU
Other Names:
BALB/NZB mtDNA heteroplasmic

Gene Information

mtNZB/BinJ;BALB/cByJ
Name: conplastic allele
Type: Allele
Species: Mus musculus (mouse)
Chromosome: Chr Mt
Genetic Alterations
Conplastic strain holding BALB/c nuclear DNA and NZB mitochondrial DNA.
Phenotype
None/Normal/Wild-type
MeSH Terms
  • Animals
  • DNA, Mitochondrial/genetics
  • Female
  • Founder Effect
  • Gene Dosage
  • Gene Frequency
  • Genotype
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred NZB
  • Oocytes
  • Oogenesis/genetics
  • Oogonia
  • Cell Nucleus/genetics
  • Cell Nucleus/metabolism
  • Chromosome Segregation
  • Crosses, Genetic
  • Genetic Linkage
  • Genetic Variation
  • Kidney/physiology
  • Liver/physiology
  • Male
  • Mice, Inbred C57BL
  • Mice, Transgenic/genetics
  • Models, Genetic
  • Muscle, Skeletal/physiology
  • Organ Specificity
  • Oxidative Phosphorylation
  • Quantitative Trait Loci
  • Selection, Genetic
  • Spleen/physiology
  • Stem Cells/physiology
  • Cell Line
  • DNA Breaks, Double-Stranded
  • DNA Repair
  • DNA, Mitochondrial/analysis
  • DNA, Mitochondrial/chemistry
  • Deoxyribonucleases, Type II Site-Specific/genetics
  • Deoxyribonucleases, Type II Site-Specific/metabolism
  • Haplotypes
  • Hepatocytes/chemistry
  • Mitochondria, Liver/enzymology
  • Polymerase Chain Reaction
  • Recombination, Genetic
  • Adenoviridae/genetics
  • Ataxin-1
  • Ataxins
  • Chimera
  • DNA Cleavage
  • DNA Restriction Enzymes/genetics
  • DNA Restriction Enzymes/metabolism
  • Gene Deletion
  • Gene Expression
  • Genetic Engineering
  • Genetic Therapy/methods
  • Genetic Vectors/administration & dosage
  • Genetic Vectors/genetics
  • Heterozygote
  • Immunoblotting
  • Immunohistochemistry
  • Injections, Intramuscular
  • Injections, Intravenous
  • Mitochondria, Liver/metabolism
  • Mitochondria, Muscle/metabolism
  • Mitochondrial Diseases/genetics
  • Mitochondrial Diseases/therapy
  • Nerve Tissue Proteins/genetics
  • Nuclear Proteins/genetics
  • Polymorphism, Restriction Fragment Length
  • Aging/immunology
  • Chromosome Segregation/immunology
  • DNA, Mitochondrial/immunology
  • Hematopoietic Stem Cells/immunology
  • Major Histocompatibility Complex/genetics
  • Mice, Inbred Strains
  • Mitochondria/genetics
  • Mitochondria/immunology
  • DNA Restriction Enzymes/administration & dosage
  • DNA, Mitochondrial/metabolism
  • Dependovirus/genetics
  • Drug Delivery Systems
  • Genetic Vectors
  • Mitochondria, Heart
  • Mutation
Strain Development
NZB cytoplasts were fused with BALB/c one-cell embryos to produce single, heteroplasmic cells that combine mitrochondrial DNA from NZB/BlNJ (RRID:IMSR_JAX:000684) and BALB/cByJ (RRID:IMSR_JAX:001026), which were implanted into B6C3F1 pseudopregnant females. Since then, the line has been backcrossed to BALB/cByJ *males* (to retain the heteroplasmy; mtDNA is inherited purely maternally) >50 times.
Suggested Control Mice
BALB/c
  • Cell Biology
  • Metabolism
  • Models for Human Disease
  • Neurobiology
  • Obesity
  • Research Tools
Donor
Eric Shoubridge, Ph.D., McGill University.
Carlos T. Moraes, Ph.D., University of Miami, Miller School of Medicine.
Primary Reference

Jenuth JP, Peterson AC, Fu K, Shoubridge EA. Random genetic drift in thefemale germline explains the rapid segregation of mammalian mitochondrial DNA.Nat Genet. 1996 Oct;14(2):146-51. (Medline PMID: 8841183)

Battersby BJ, Loredo-Osti JC, Shoubridge EA. Nuclear genetic control ofmitochondrial DNA segregation. Nat Genet. 2003 Feb;33(2):183-6. Epub 2003 Jan 21.(Medline PMID: 12539044)

Bacman SR, Williams SL, Moraes CT. Intra- and inter-molecular recombination ofmitochondrial DNA after in vivo induction of multiple double-strand breaks.Nucleic Acids Res. 2009 Jul;37(13):4218-26. doi: 10.1093/nar/gkp348. Epub 2009May 12. (Medline PMID: 19435881)

Bacman SR, Williams SL, Hernandez D, Moraes CT. Modulating mtDNA heteroplasmy by mitochondria-targeted restriction endonucleases in a 'differential multiplecleavage-site' model. Gene Ther. 2007 Sep;14(18):1309-18. Epub 2007 Jun 28.(Medline PMID: 17597792)

Battersby BJ, Redpath ME, Shoubridge EA. Mitochondrial DNA segregation inhematopoietic lineages does not depend on MHC presentation of mitochondriallyencoded peptides. Hum Mol Genet. 2005 Sep 1;14(17):2587-94. Epub 2005 Jul 27.(Medline PMID: 16049030)

Bacman SR, Williams SL, Garcia S, Moraes CT. Organ-specific shifts in mtDNAheteroplasmy following systemic delivery of a mitochondria-targeted restrictionendonuclease. Gene Ther. 2010 Jun;17(6):713-20. doi: 10.1038/gt.2010.25. Epub2010 Mar 11. (Medline PMID: 20220783)

Colony and Husbandry Information

Females (with high NZB mtDNA) are bred with BALB/c males

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
White
Eye
Black
MMRRC Breeding System
Backcross
Generation
Nuclear background is BALB/c (N50+)
Overall Breeding Performance
Good
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
4 to 6
Recommended wean age
3 Weeks
Average Pups Weaned
4 to 6

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
067057-MU-EMBRYO Cryo-preserved embryos $1,038.00 / $2,621.00
Non-Profit / For-Profit
Aliquot Approximate quantity2 : 20-40 embryos / aliquot
067057-MU-RESUS Litter recovered from cryo-archive $2,028.00 / $4,127.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.