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Availability & Fees Order this Strain
SM22 knockout, TaglnTMlili, SM22TMlili , SM22KO-nlacZ
"SV129 derived ES cells" per PMID:20478474, which could be any 129S or 129X substrain
Heterozygotes and homozygotes appear normal, but stress conditions (e.g., oxidative stress, inflammation, and chondrogenesis) induce artery vessel wall remodeling. Sometimes, pregnant SM22 females may experience dystocia.
The targeting vector was injected into 129/Sv-derived ES cells. G418-resistant ES clones were genotyped via PCR and Southern blotting. Heterozygotes were mated with B6 and 129/Sv for at least 4 generations, and the line was maintained in this mixed genetic background.
Yang M, Jiang H, Li L. Sm22α transcription occurs at the early onset of the cardiovascular system and the intron 1 is dispensable for its transcription in smooth muscle cells during mouse development. Int J Physiol Pathophysiol Pharmacol. 2010;2(1):12-9. Epub 2009 Nov 22. (Medline PMID: 20428474)
Shen J, Yang M, Ju D, Jiang H, Zheng JP, Xu Z, Li L. Disruption of SM22 promotes inflammation after artery injury via nuclear factor kappaB activation. Circ Res. 2010 Apr 30;106(8):1351-62. doi: 10.1161/CIRCRESAHA.109.213900. Epub 2010 Mar 11. (Medline PMID: 20224039)
Shen J, Yang M, Jiang H, Ju D, Zheng JP, Xu Z, Liao TD, Li L. Arterial injury promotes medial chondrogenesis in Sm22 knockout mice. Cardiovasc Res. 2011 Apr 1;90(1):28-37. doi: 10.1093/cvr/cvq378. Epub 2010 Dec 22. (Medline PMID: 21183509)
Wu SP, Dong XR, Regan JN, Su C, Majesky MW. Tbx18 regulates development of the epicardium and coronary vessels. Dev Biol. 2013 Nov 15;383(2):307-20. doi: 10.1016/j.ydbio.2013.08.019. Epub 2013 Sep 7. (Medline PMID: 24016759)
Hu J, Xie C, Ma H, Yang B, Ma PX, Chen YE. Construction of vascular tissues with macro-porous nano-fibrous scaffolds and smooth muscle cells enriched from differentiated embryonic stem cells. PLoS One. 2012;7(4):e35580. doi: 10.1371/journal.pone.0035580. Epub 2012 Apr 24. (Medline PMID: 22545119)
Colony Surveillance Program and Current Health Reports
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
A Commercial License Agreement from the Donor is required for for-profit entities to use this strain.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.